Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma

Abstract Background Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with high mortality worldwide. Accumulating researches have indicated that long non‑coding RNAs (lncRNAs) are involved in varies human cancers, including HCC. Nevertheless, the specific molecular mechanism...

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Autores principales:	ZhenYu Feng, ZhenYu Ye, JiaMing Xie, Wei Chen, Wei Li, ChunGen Xing
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Hepatocellular carcinoma LOXL1-AS1 miR-3614-5p YY1 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/81b5776956924c9880a35fff1b2114a5
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spelling	oai:doaj.org-article:81b5776956924c9880a35fff1b2114a52021-12-05T12:06:09ZStudy on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma10.1186/s13062-021-00312-81745-6150https://doaj.org/article/81b5776956924c9880a35fff1b2114a52021-12-01T00:00:00Zhttps://doi.org/10.1186/s13062-021-00312-8https://doaj.org/toc/1745-6150Abstract Background Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with high mortality worldwide. Accumulating researches have indicated that long non‑coding RNAs (lncRNAs) are involved in varies human cancers, including HCC. Nevertheless, the specific molecular mechanism of lncRNA lysyl oxidase like 1 antisense RNA 1 (LOXL1-AS1) in HCC is still unclear. Methods LOXL1-AS1 expression was tested via qRT-PCR in HCC cells. Functional and mechanism assays were respectively done to evaluate the biological functions of HCC cells and the potential interaction of LOXL1-AS1 and other factors. Results We discovered that LOXL1-AS1 was high expressed in HCC cells. Inhibition of LOXL1-AS1 repressed cell proliferation, migration and invasion, but enhanced cell apoptosis in HCC. Further, miR-3614-5p was proven to be sponged by LOXL1-AS1. Additionally, Yin Yang 1 (YY1) was proven as the target gene of miR-3614-5p, and YY1 depletion could repress HCC cell malignant behaviors. YY1 could also transcriptionally activate LOXL1-AS1 expression. In rescue assays, we confirmed that overexpression of YY1 or miR-3614-5p inhibition could reverse the suppressive effects of LOXL1-AS1 silence on the malignant behaviors of HCC cells. Conclusion In short, LOXL1-AS1/miR-3614-5p/YY1 forms a positive loop in modulating HCC cell malignant behaviors.ZhenYu FengZhenYu YeJiaMing XieWei ChenWei LiChunGen XingBMCarticleHepatocellular carcinomaLOXL1-AS1miR-3614-5pYY1Biology (General)QH301-705.5ENBiology Direct, Vol 16, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatocellular carcinoma LOXL1-AS1 miR-3614-5p YY1 Biology (General) QH301-705.5
spellingShingle	Hepatocellular carcinoma LOXL1-AS1 miR-3614-5p YY1 Biology (General) QH301-705.5 ZhenYu Feng ZhenYu Ye JiaMing Xie Wei Chen Wei Li ChunGen Xing Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
description	Abstract Background Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with high mortality worldwide. Accumulating researches have indicated that long non‑coding RNAs (lncRNAs) are involved in varies human cancers, including HCC. Nevertheless, the specific molecular mechanism of lncRNA lysyl oxidase like 1 antisense RNA 1 (LOXL1-AS1) in HCC is still unclear. Methods LOXL1-AS1 expression was tested via qRT-PCR in HCC cells. Functional and mechanism assays were respectively done to evaluate the biological functions of HCC cells and the potential interaction of LOXL1-AS1 and other factors. Results We discovered that LOXL1-AS1 was high expressed in HCC cells. Inhibition of LOXL1-AS1 repressed cell proliferation, migration and invasion, but enhanced cell apoptosis in HCC. Further, miR-3614-5p was proven to be sponged by LOXL1-AS1. Additionally, Yin Yang 1 (YY1) was proven as the target gene of miR-3614-5p, and YY1 depletion could repress HCC cell malignant behaviors. YY1 could also transcriptionally activate LOXL1-AS1 expression. In rescue assays, we confirmed that overexpression of YY1 or miR-3614-5p inhibition could reverse the suppressive effects of LOXL1-AS1 silence on the malignant behaviors of HCC cells. Conclusion In short, LOXL1-AS1/miR-3614-5p/YY1 forms a positive loop in modulating HCC cell malignant behaviors.
format	article
author	ZhenYu Feng ZhenYu Ye JiaMing Xie Wei Chen Wei Li ChunGen Xing
author_facet	ZhenYu Feng ZhenYu Ye JiaMing Xie Wei Chen Wei Li ChunGen Xing
author_sort	ZhenYu Feng
title	Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
title_short	Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
title_full	Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
title_fullStr	Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
title_full_unstemmed	Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
title_sort	study on the mechanism of loxl1-as1/mir-3614-5p/yy1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/81b5776956924c9880a35fff1b2114a5
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Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma

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