Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis

Abstract Background Visceral Leishmaniasis (VL) is a severely neglected disease affecting millions of people with high mortality if left untreated. In Ethiopia, the primary laboratory diagnosis of VL is by using an antigen from a 39-amino acid sequence repeat of a kinesin-related (rK39) of leishmani...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dawit Gebreegziabiher Hagos, Henk D. F. H. Schallig, Yazezew K. Kiros, Mahmud Abdulkadir, Dawit Wolday
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	VL rk39 Sensitivity Specificity Ethiopia Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/81b595a36d9e4cb5a234f4754e315376
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:81b595a36d9e4cb5a234f4754e315376
record_format	dspace
spelling	oai:doaj.org-article:81b595a36d9e4cb5a234f4754e3153762021-11-21T12:42:45ZPerformance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis10.1186/s12879-021-06826-w1471-2334https://doaj.org/article/81b595a36d9e4cb5a234f4754e3153762021-11-01T00:00:00Zhttps://doi.org/10.1186/s12879-021-06826-whttps://doaj.org/toc/1471-2334Abstract Background Visceral Leishmaniasis (VL) is a severely neglected disease affecting millions of people with high mortality if left untreated. In Ethiopia, the primary laboratory diagnosis of VL is by using an antigen from a 39-amino acid sequence repeat of a kinesin-related (rK39) of leishmania donovani complex (L. donovani), rapid diagnostic tests (RDT). Different rk39 RDT brands are available with very variable performance and studies from Ethiopia showed a very wide range of sensitivity and specificity. Therefore, a systematic review and meta-analysis were conducted to determine the pooled sensitivity and specificity of rk39 RDT in Ethiopia. Method PUBMED, EMBASE, and other sources were searched using predefined search terms to retrieve all relevant articles from 2007 to 2020. Heterogeneity was assessed by visually inspecting summary receiver operating curves (SROC), Spearman correlation coefficient (rs), Cochran Q test statistics, inconsistency square (I2) and subgroup analysis. The presence and statistical significance of publication bias were assessed by Egger's test at p < 0.05, and all the measurements showed the presence of considerable heterogeneity. Quality assessment of diagnostic accuracy studies (QUADAS-2) checklists was used to check the qualities of the study. Results A total of 664 articles were retrieved, and of this 12 articles were included in the meta-analysis. Overall pooled sensitivity and specificity of the rk39 RDT to diagnose VL in Ethiopia were 88.0% (95% CI 86.0% to 89.0%) and 84.0% (95% CI 82.0% to 86.0%), respectively. The sensitivity and specificity of the rk39 RDT commercial test kits were DiaMed: 86.9% (95% CI 84.3% to 89.1%) and 82.2% (95% CI 79.3% to 85.0%), and InBios: 80.0% (95% CI 77.0% to 82.8%) and 97.4% (95% CI 95.0% to 98.8%), respectively. Conclusion Referring to our result, rk39 RDT considered an essential rapid diagnostic test for VL diagnosis. Besides to the diagnostic accuracy, the features such as easy to perform, quick (10–20 min), cheap, equipment-free, electric and cold chain free, and result reproducibility, rk39 RDT is advisable to remains in practice as a diagnostic test at least in the remote VL endemic localities till a better test will come.Dawit Gebreegziabiher HagosHenk D. F. H. SchalligYazezew K. KirosMahmud AbdulkadirDawit WoldayBMCarticleVLrk39SensitivitySpecificityEthiopiaInfectious and parasitic diseasesRC109-216ENBMC Infectious Diseases, Vol 21, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	VL rk39 Sensitivity Specificity Ethiopia Infectious and parasitic diseases RC109-216
spellingShingle	VL rk39 Sensitivity Specificity Ethiopia Infectious and parasitic diseases RC109-216 Dawit Gebreegziabiher Hagos Henk D. F. H. Schallig Yazezew K. Kiros Mahmud Abdulkadir Dawit Wolday Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis
description	Abstract Background Visceral Leishmaniasis (VL) is a severely neglected disease affecting millions of people with high mortality if left untreated. In Ethiopia, the primary laboratory diagnosis of VL is by using an antigen from a 39-amino acid sequence repeat of a kinesin-related (rK39) of leishmania donovani complex (L. donovani), rapid diagnostic tests (RDT). Different rk39 RDT brands are available with very variable performance and studies from Ethiopia showed a very wide range of sensitivity and specificity. Therefore, a systematic review and meta-analysis were conducted to determine the pooled sensitivity and specificity of rk39 RDT in Ethiopia. Method PUBMED, EMBASE, and other sources were searched using predefined search terms to retrieve all relevant articles from 2007 to 2020. Heterogeneity was assessed by visually inspecting summary receiver operating curves (SROC), Spearman correlation coefficient (rs), Cochran Q test statistics, inconsistency square (I2) and subgroup analysis. The presence and statistical significance of publication bias were assessed by Egger's test at p < 0.05, and all the measurements showed the presence of considerable heterogeneity. Quality assessment of diagnostic accuracy studies (QUADAS-2) checklists was used to check the qualities of the study. Results A total of 664 articles were retrieved, and of this 12 articles were included in the meta-analysis. Overall pooled sensitivity and specificity of the rk39 RDT to diagnose VL in Ethiopia were 88.0% (95% CI 86.0% to 89.0%) and 84.0% (95% CI 82.0% to 86.0%), respectively. The sensitivity and specificity of the rk39 RDT commercial test kits were DiaMed: 86.9% (95% CI 84.3% to 89.1%) and 82.2% (95% CI 79.3% to 85.0%), and InBios: 80.0% (95% CI 77.0% to 82.8%) and 97.4% (95% CI 95.0% to 98.8%), respectively. Conclusion Referring to our result, rk39 RDT considered an essential rapid diagnostic test for VL diagnosis. Besides to the diagnostic accuracy, the features such as easy to perform, quick (10–20 min), cheap, equipment-free, electric and cold chain free, and result reproducibility, rk39 RDT is advisable to remains in practice as a diagnostic test at least in the remote VL endemic localities till a better test will come.
format	article
author	Dawit Gebreegziabiher Hagos Henk D. F. H. Schallig Yazezew K. Kiros Mahmud Abdulkadir Dawit Wolday
author_facet	Dawit Gebreegziabiher Hagos Henk D. F. H. Schallig Yazezew K. Kiros Mahmud Abdulkadir Dawit Wolday
author_sort	Dawit Gebreegziabiher Hagos
title	Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis
title_short	Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis
title_full	Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis
title_fullStr	Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis
title_full_unstemmed	Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis
title_sort	performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in ethiopia: a systematic review and meta-analysis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/81b595a36d9e4cb5a234f4754e315376
work_keys_str_mv	AT dawitgebreegziabiherhagos performanceofrapidrk39testsforthediagnosisofvisceralleishmaniasisinethiopiaasystematicreviewandmetaanalysis AT henkdfhschallig performanceofrapidrk39testsforthediagnosisofvisceralleishmaniasisinethiopiaasystematicreviewandmetaanalysis AT yazezewkkiros performanceofrapidrk39testsforthediagnosisofvisceralleishmaniasisinethiopiaasystematicreviewandmetaanalysis AT mahmudabdulkadir performanceofrapidrk39testsforthediagnosisofvisceralleishmaniasisinethiopiaasystematicreviewandmetaanalysis AT dawitwolday performanceofrapidrk39testsforthediagnosisofvisceralleishmaniasisinethiopiaasystematicreviewandmetaanalysis
_version_	1718418834491179008

Performance of rapid rk39 tests for the diagnosis of visceral leishmaniasis in Ethiopia: a systematic review and meta-analysis

Ejemplares similares