Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita

Abstract. Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687. The endophytic bacteria from spices plants have potential a...

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Autores principales:	WIRATNO WIRATNO, MUHAMMAD SYAKIR, IRWANTO SUCIPTO, ANKARDIANSYAH PANDU PRADANA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/81ba77d454424b85910d2d9c43e0f9bc
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spelling	oai:doaj.org-article:81ba77d454424b85910d2d9c43e0f9bc2021-11-16T14:12:15ZIsolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita1412-033X2085-472210.13057/biodiv/d200310https://doaj.org/article/81ba77d454424b85910d2d9c43e0f9bc2019-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3570https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687. The endophytic bacteria from spices plants have potential as biocontrol agents. Nevertheless, their potential has not been explored. Pepper plant (Piper nigrum L.) is linked with promising endophytic bacteria as biocontrol agents. This study aimed to obtain isolates of endophytic bacteria from root tissues of pepper plant. The isolation of endophytic bacteria was done using surface-sterilization method and using Tryptone Soya Agar (TSA) medium. The result showed that 10 endophytic bacteria were successfully isolated. We found that a total of 9 bacteria were safe for plants and mammals and used for subsequent steps. The selected bacteria showed inhibition activity of Fusarium oxysporum (18.6 to 43.7%). Furthermore, the secondary metabolites of the endophytic bacteria also promoted lethal effects on Meloidogyne incognita (16.6 to 65.8%). The physiological activity also showed that seven isolates were able to produce chitinase, and four (4) isolates were able to produce protease. Additionally, 55.6% of isolates were also able to dissolve phosphorus and fix nitrogen. This study provided fundamental information related to the biocontrol properties of endophytic bacteria isolated from pepper plant roots.WIRATNO WIRATNOMUHAMMAD SYAKIRIRWANTO SUCIPTOANKARDIANSYAH PANDU PRADANAMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 3, Pp 682-687 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 WIRATNO WIRATNO MUHAMMAD SYAKIR IRWANTO SUCIPTO ANKARDIANSYAH PANDU PRADANA Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita
description	Abstract. Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687. The endophytic bacteria from spices plants have potential as biocontrol agents. Nevertheless, their potential has not been explored. Pepper plant (Piper nigrum L.) is linked with promising endophytic bacteria as biocontrol agents. This study aimed to obtain isolates of endophytic bacteria from root tissues of pepper plant. The isolation of endophytic bacteria was done using surface-sterilization method and using Tryptone Soya Agar (TSA) medium. The result showed that 10 endophytic bacteria were successfully isolated. We found that a total of 9 bacteria were safe for plants and mammals and used for subsequent steps. The selected bacteria showed inhibition activity of Fusarium oxysporum (18.6 to 43.7%). Furthermore, the secondary metabolites of the endophytic bacteria also promoted lethal effects on Meloidogyne incognita (16.6 to 65.8%). The physiological activity also showed that seven isolates were able to produce chitinase, and four (4) isolates were able to produce protease. Additionally, 55.6% of isolates were also able to dissolve phosphorus and fix nitrogen. This study provided fundamental information related to the biocontrol properties of endophytic bacteria isolated from pepper plant roots.
format	article
author	WIRATNO WIRATNO MUHAMMAD SYAKIR IRWANTO SUCIPTO ANKARDIANSYAH PANDU PRADANA
author_facet	WIRATNO WIRATNO MUHAMMAD SYAKIR IRWANTO SUCIPTO ANKARDIANSYAH PANDU PRADANA
author_sort	WIRATNO WIRATNO
title	Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita
title_short	Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita
title_full	Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita
title_fullStr	Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita
title_full_unstemmed	Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita
title_sort	isolation and characterization of endophytic bacteria from roots of piper nigrum and their activities against fusarium oxysporum and meloidogyne incognita
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/81ba77d454424b85910d2d9c43e0f9bc
work_keys_str_mv	AT wiratnowiratno isolationandcharacterizationofendophyticbacteriafromrootsofpipernigrumandtheiractivitiesagainstfusariumoxysporumandmeloidogyneincognita AT muhammadsyakir isolationandcharacterizationofendophyticbacteriafromrootsofpipernigrumandtheiractivitiesagainstfusariumoxysporumandmeloidogyneincognita AT irwantosucipto isolationandcharacterizationofendophyticbacteriafromrootsofpipernigrumandtheiractivitiesagainstfusariumoxysporumandmeloidogyneincognita AT ankardiansyahpandupradana isolationandcharacterizationofendophyticbacteriafromrootsofpipernigrumandtheiractivitiesagainstfusariumoxysporumandmeloidogyneincognita
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Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita

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