Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein
Piotr Celejewski-Marciniak,1 Renata Wolinowska,2 Marta Wróblewska1,3 1Department of Dental Microbiology, Medical University of Warsaw, Warsaw, Poland; 2Department of Pharmaceutical Microbiology, Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland; 3Department of Microbiolog...
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Dove Medical Press
2021
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oai:doaj.org-article:81be39b3d28f4a348dd07656de52692d2021-11-04T19:00:26ZMolecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein1178-6973https://doaj.org/article/81be39b3d28f4a348dd07656de52692d2021-11-01T00:00:00Zhttps://www.dovepress.com/molecular-characterization-of-class-1-2-and-3-integrons-in-serratia-sp-peer-reviewed-fulltext-article-IDRhttps://doaj.org/toc/1178-6973Piotr Celejewski-Marciniak,1 Renata Wolinowska,2 Marta Wróblewska1,3 1Department of Dental Microbiology, Medical University of Warsaw, Warsaw, Poland; 2Department of Pharmaceutical Microbiology, Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland; 3Department of Microbiology, Central Clinical Hospital, University Clinical Centre, Medical University of Warsaw, Warsaw, PolandCorrespondence: Marta Wróblewska; Piotr Celejewski-MarciniakDepartment of Dental Microbiology, Medical University of Warsaw, 1a Banacha street, Warsaw, 02-097, PolandTel +48225991777; +48665101188Email marta.wroblewska@wum.edu.pl; piotrcm@onet.plPurpose: Gram-negative rods of the genus Serratia play an increasing role as etiological agents of healthcare-associated infections (HAI) in humans. These bacteria are characterized by natural and acquired resistance to several groups of antibacterial agents. The aim of the study was to characterize class 1, 2 and 3 integrons in the clinical isolates of Serratia spp. in Poland.Methods: The study comprised 112 clinical strains of Serratia, isolated from patients hospitalized in Poland in 2010– 2012. Identification of strains was confirmed using MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) system. Detection of class 1, 2 and 3 integrase DNA sequence was performed by multiplex-PCR. Amplicons obtained in the PCR reactions were purified and then sequenced bidirectionally.Results: Among the analyzed strains, Serratia marcescens was a predominant species (103/112, 92.0%). All three classes of integrase DNA sequence were detected in the analyzed strains of Serratia spp. DNA sequence of class 3 integron, besides integrase gene, revealed three gene cassettes (dfrB3, blaGES-7,blaOXA/aac(6ʹ)-Ib-cr). BLAST analysis of DNA sequence revealed that class 3 integron was carried on 9448 bp plasmid which was named pPCMI3 – whole sequence of its DNA was submitted to GenBank NCBI (National Center for Biotechnology Information) – NCBI MH569711.Conclusion: In this study, we identified a new plasmid pPCMI3 harboring class 3 integron. This is the first report of a gene oxa/aac(6ʹ)-Ib-cr coding for a novel fusion protein, which consists of OXA β-lactamase and acetyltransferase aac(6ʹ)-Ib-cr. In the analyzed strains, class 1 and 2 integrons were also detected. Among the strains with class 1 integron, nine contained cassette array 5ʹCS-aadA2-ORF-dfrA12-3ʹCS, and two – cassette array 5ʹCS-aacC1-ORF-ORF-aadA1-3ʹCS, which were not previously reported in Serratia spp.Keywords: Serratia marcescens, gene cassettes, antibiotic resistance, carbapenemase, fusion proteinCelejewski-Marciniak PWolinowska RWróblewska MDove Medical Pressarticleserratia marcescensgene cassettesantibiotic resistancecarbapenemasefusion proteinInfectious and parasitic diseasesRC109-216ENInfection and Drug Resistance, Vol Volume 14, Pp 4601-4610 (2021) |
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serratia marcescens gene cassettes antibiotic resistance carbapenemase fusion protein Infectious and parasitic diseases RC109-216 |
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serratia marcescens gene cassettes antibiotic resistance carbapenemase fusion protein Infectious and parasitic diseases RC109-216 Celejewski-Marciniak P Wolinowska R Wróblewska M Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein |
| description |
Piotr Celejewski-Marciniak,1 Renata Wolinowska,2 Marta Wróblewska1,3 1Department of Dental Microbiology, Medical University of Warsaw, Warsaw, Poland; 2Department of Pharmaceutical Microbiology, Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland; 3Department of Microbiology, Central Clinical Hospital, University Clinical Centre, Medical University of Warsaw, Warsaw, PolandCorrespondence: Marta Wróblewska; Piotr Celejewski-MarciniakDepartment of Dental Microbiology, Medical University of Warsaw, 1a Banacha street, Warsaw, 02-097, PolandTel +48225991777; +48665101188Email marta.wroblewska@wum.edu.pl; piotrcm@onet.plPurpose: Gram-negative rods of the genus Serratia play an increasing role as etiological agents of healthcare-associated infections (HAI) in humans. These bacteria are characterized by natural and acquired resistance to several groups of antibacterial agents. The aim of the study was to characterize class 1, 2 and 3 integrons in the clinical isolates of Serratia spp. in Poland.Methods: The study comprised 112 clinical strains of Serratia, isolated from patients hospitalized in Poland in 2010– 2012. Identification of strains was confirmed using MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) system. Detection of class 1, 2 and 3 integrase DNA sequence was performed by multiplex-PCR. Amplicons obtained in the PCR reactions were purified and then sequenced bidirectionally.Results: Among the analyzed strains, Serratia marcescens was a predominant species (103/112, 92.0%). All three classes of integrase DNA sequence were detected in the analyzed strains of Serratia spp. DNA sequence of class 3 integron, besides integrase gene, revealed three gene cassettes (dfrB3, blaGES-7,blaOXA/aac(6ʹ)-Ib-cr). BLAST analysis of DNA sequence revealed that class 3 integron was carried on 9448 bp plasmid which was named pPCMI3 – whole sequence of its DNA was submitted to GenBank NCBI (National Center for Biotechnology Information) – NCBI MH569711.Conclusion: In this study, we identified a new plasmid pPCMI3 harboring class 3 integron. This is the first report of a gene oxa/aac(6ʹ)-Ib-cr coding for a novel fusion protein, which consists of OXA β-lactamase and acetyltransferase aac(6ʹ)-Ib-cr. In the analyzed strains, class 1 and 2 integrons were also detected. Among the strains with class 1 integron, nine contained cassette array 5ʹCS-aadA2-ORF-dfrA12-3ʹCS, and two – cassette array 5ʹCS-aacC1-ORF-ORF-aadA1-3ʹCS, which were not previously reported in Serratia spp.Keywords: Serratia marcescens, gene cassettes, antibiotic resistance, carbapenemase, fusion protein |
| format |
article |
| author |
Celejewski-Marciniak P Wolinowska R Wróblewska M |
| author_facet |
Celejewski-Marciniak P Wolinowska R Wróblewska M |
| author_sort |
Celejewski-Marciniak P |
| title |
Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein |
| title_short |
Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein |
| title_full |
Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein |
| title_fullStr |
Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein |
| title_full_unstemmed |
Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein |
| title_sort |
molecular characterization of class 1, 2 and 3 integrons in serratia spp. clinical isolates in poland – isolation of a new plasmid and identification of a gene for a novel fusion protein |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/81be39b3d28f4a348dd07656de52692d |
| work_keys_str_mv |
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| _version_ |
1718444661964537856 |