Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content

Infections due to Staphylococcus argenteus have been increasingly reported worldwide and the microbe cannot be distinguished from Staphylococcus aureus by standard methods. Its complement of virulence determinants and antibiotic resistance genes remain unclear, and how far these are distinct from th...

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Autores principales:	Cosmika Goswami, Stephen Fox, Matthew Holden, Alistair Leanord, Thomas J. Evans
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Staphylococcus argenteus pathogenicity islands Staphylococcus argenteus antibiotic resistance virulence genes CRISPR/Cas Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/81c12547ccd14cf19d211a1312f1ea3f
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spelling	oai:doaj.org-article:81c12547ccd14cf19d211a1312f1ea3f2021-12-02T11:37:12ZGenomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content1664-302X10.3389/fmicb.2021.795173https://doaj.org/article/81c12547ccd14cf19d211a1312f1ea3f2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.795173/fullhttps://doaj.org/toc/1664-302XInfections due to Staphylococcus argenteus have been increasingly reported worldwide and the microbe cannot be distinguished from Staphylococcus aureus by standard methods. Its complement of virulence determinants and antibiotic resistance genes remain unclear, and how far these are distinct from those produced by S. aureus remains undetermined. In order to address these uncertainties, we have collected 132 publicly available sequences from fourteen different countries, including the United Kingdom, between 2005 and 2018 to study the global genetic structure of the population. We have compared the genomes for antibiotic resistance genes, virulence determinants and mobile genetic elements such as phages, pathogenicity islands and presence of plasmid groups between different clades. 20% (n = 26) isolates were methicillin resistant harboring a mecA gene and 88% were penicillin resistant, harboring the blaZ gene. ST2250 was identified as the most frequent strain, but ST1223, which was the second largest group, contained a marginally larger number of virulence genes compared to the other STs. Novel S. argenteus pathogenicity islands were identified in our isolates harboring tsst-1, seb, sec3, ear, selk, selq toxin genes, as well as chromosomal clusters of enterotoxin and superantigen-like genes. Strain-specific type I modification systems were widespread which would limit interstrain transfer of genetic material. In addition, ST2250 possessed a CRISPR/Cas system, lacking in most other STs. S. argenteus possesses important genetic differences from S. aureus, as well as between different STs, with the potential to produce distinct clinical manifestations.Cosmika GoswamiStephen FoxMatthew HoldenAlistair LeanordAlistair LeanordThomas J. EvansFrontiers Media S.A.articleStaphylococcus argenteus pathogenicity islandsStaphylococcus argenteusantibiotic resistancevirulence genesCRISPR/CasMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Staphylococcus argenteus pathogenicity islands Staphylococcus argenteus antibiotic resistance virulence genes CRISPR/Cas Microbiology QR1-502
spellingShingle	Staphylococcus argenteus pathogenicity islands Staphylococcus argenteus antibiotic resistance virulence genes CRISPR/Cas Microbiology QR1-502 Cosmika Goswami Stephen Fox Matthew Holden Alistair Leanord Alistair Leanord Thomas J. Evans Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content
description	Infections due to Staphylococcus argenteus have been increasingly reported worldwide and the microbe cannot be distinguished from Staphylococcus aureus by standard methods. Its complement of virulence determinants and antibiotic resistance genes remain unclear, and how far these are distinct from those produced by S. aureus remains undetermined. In order to address these uncertainties, we have collected 132 publicly available sequences from fourteen different countries, including the United Kingdom, between 2005 and 2018 to study the global genetic structure of the population. We have compared the genomes for antibiotic resistance genes, virulence determinants and mobile genetic elements such as phages, pathogenicity islands and presence of plasmid groups between different clades. 20% (n = 26) isolates were methicillin resistant harboring a mecA gene and 88% were penicillin resistant, harboring the blaZ gene. ST2250 was identified as the most frequent strain, but ST1223, which was the second largest group, contained a marginally larger number of virulence genes compared to the other STs. Novel S. argenteus pathogenicity islands were identified in our isolates harboring tsst-1, seb, sec3, ear, selk, selq toxin genes, as well as chromosomal clusters of enterotoxin and superantigen-like genes. Strain-specific type I modification systems were widespread which would limit interstrain transfer of genetic material. In addition, ST2250 possessed a CRISPR/Cas system, lacking in most other STs. S. argenteus possesses important genetic differences from S. aureus, as well as between different STs, with the potential to produce distinct clinical manifestations.
format	article
author	Cosmika Goswami Stephen Fox Matthew Holden Alistair Leanord Alistair Leanord Thomas J. Evans
author_facet	Cosmika Goswami Stephen Fox Matthew Holden Alistair Leanord Alistair Leanord Thomas J. Evans
author_sort	Cosmika Goswami
title	Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content
title_short	Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content
title_full	Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content
title_fullStr	Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content
title_full_unstemmed	Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content
title_sort	genomic analysis of global staphylococcus argenteus strains reveals distinct lineages with differing virulence and antibiotic resistance gene content
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/81c12547ccd14cf19d211a1312f1ea3f
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Genomic Analysis of Global Staphylococcus argenteus Strains Reveals Distinct Lineages With Differing Virulence and Antibiotic Resistance Gene Content

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