Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing

Abstract The aim of this study was to examine the regulation of transcriptomics and proteomics related to the yellowing of fresh-cut yams after storage. The comparison of yellow fresh-cut yam (YFY) vs. white fresh-cut yam (control) revealed 6894 upregulated and 6800 downregulated differentially expr...

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Autores principales:	Shuang Guo, Dan Wang, Yue Ma, Yan Zhang, Xiaoyan Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81c5dd3f7e024bdd956ed63215fb1031
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spelling	oai:doaj.org-article:81c5dd3f7e024bdd956ed63215fb10312021-12-02T14:17:27ZCombination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing10.1038/s41598-021-87423-42045-2322https://doaj.org/article/81c5dd3f7e024bdd956ed63215fb10312021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87423-4https://doaj.org/toc/2045-2322Abstract The aim of this study was to examine the regulation of transcriptomics and proteomics related to the yellowing of fresh-cut yams after storage. The comparison of yellow fresh-cut yam (YFY) vs. white fresh-cut yam (control) revealed 6894 upregulated and 6800 downregulated differentially expressed genes along with 1277 upregulated and 677 downregulated differentially expressed proteins. The results showed that the total carotenoids, flavonoids, and bisdemethoxycurcumin in YFY were higher than in the control due to the significant up-regulation of critical genes in the carotenoid biosynthesis pathway, flavonoid biosynthesis pathway, and stilbenoid, diarylheptanoid, and gingerol biosynthesis pathway. In addition, the tricarboxylic acid cycle and phenylpropanoid biosynthesis were both enhanced in YFY compared to the control, providing energy and precursors for the formation of yellow pigments. The results suggest that the synthesis of yellow pigments is regulated by critical genes, which might explain the yellowing of fresh-cut yam after storage.Shuang GuoDan WangYue MaYan ZhangXiaoyan ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuang Guo Dan Wang Yue Ma Yan Zhang Xiaoyan Zhao Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing
description	Abstract The aim of this study was to examine the regulation of transcriptomics and proteomics related to the yellowing of fresh-cut yams after storage. The comparison of yellow fresh-cut yam (YFY) vs. white fresh-cut yam (control) revealed 6894 upregulated and 6800 downregulated differentially expressed genes along with 1277 upregulated and 677 downregulated differentially expressed proteins. The results showed that the total carotenoids, flavonoids, and bisdemethoxycurcumin in YFY were higher than in the control due to the significant up-regulation of critical genes in the carotenoid biosynthesis pathway, flavonoid biosynthesis pathway, and stilbenoid, diarylheptanoid, and gingerol biosynthesis pathway. In addition, the tricarboxylic acid cycle and phenylpropanoid biosynthesis were both enhanced in YFY compared to the control, providing energy and precursors for the formation of yellow pigments. The results suggest that the synthesis of yellow pigments is regulated by critical genes, which might explain the yellowing of fresh-cut yam after storage.
format	article
author	Shuang Guo Dan Wang Yue Ma Yan Zhang Xiaoyan Zhao
author_facet	Shuang Guo Dan Wang Yue Ma Yan Zhang Xiaoyan Zhao
author_sort	Shuang Guo
title	Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing
title_short	Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing
title_full	Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing
title_fullStr	Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing
title_full_unstemmed	Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing
title_sort	combination of rna-seq transcriptomics and itraq proteomics reveal the mechanism involved in fresh-cut yam yellowing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/81c5dd3f7e024bdd956ed63215fb1031
work_keys_str_mv	AT shuangguo combinationofrnaseqtranscriptomicsanditraqproteomicsrevealthemechanisminvolvedinfreshcutyamyellowing AT danwang combinationofrnaseqtranscriptomicsanditraqproteomicsrevealthemechanisminvolvedinfreshcutyamyellowing AT yuema combinationofrnaseqtranscriptomicsanditraqproteomicsrevealthemechanisminvolvedinfreshcutyamyellowing AT yanzhang combinationofrnaseqtranscriptomicsanditraqproteomicsrevealthemechanisminvolvedinfreshcutyamyellowing AT xiaoyanzhao combinationofrnaseqtranscriptomicsanditraqproteomicsrevealthemechanisminvolvedinfreshcutyamyellowing
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Combination of RNA-Seq transcriptomics and iTRAQ proteomics reveal the mechanism involved in fresh-cut yam yellowing

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