Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis

Hyojin Lee,1,* Chloe Kim,2,* Dongjin Lee,1,3,* Jea Ho Park,1,2 Peter C Searson,2 Kwan Hyi Lee1,3 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; 2Department of Materials Science and Engineering, 3Department of Biom...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lee H, Kim C, Lee D, Park JH, Searson PC, Lee KH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Fusion genes Prostate cancer Quantum dots Multiplexed assay Optical detection Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/81c7ff7a464e400f84ff47c643758a22
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:81c7ff7a464e400f84ff47c643758a22
record_format	dspace
spelling	oai:doaj.org-article:81c7ff7a464e400f84ff47c643758a222021-12-02T04:54:08ZOptical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis1178-2013https://doaj.org/article/81c7ff7a464e400f84ff47c643758a222017-06-01T00:00:00Zhttps://www.dovepress.com/optical-coding-of-fusion-genes-using-multicolor-quantum-dots-for-prost-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hyojin Lee,1,* Chloe Kim,2,* Dongjin Lee,1,3,* Jea Ho Park,1,2 Peter C Searson,2 Kwan Hyi Lee1,3 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; 2Department of Materials Science and Engineering, 3Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Republic of Korea *These authors contributed equally to this work Abstract: Recent studies have found that prostate cancer expresses abnormal genetic markers including multiple types of TMPRSS2–ERG fusion genes. The expression level of different TMPRSS2–ERG fusion genes is correlated to pathologic variables of aggressive prostate cancer and disease progression. State-of-the-art methods for detection of TMPRSS2–ERG fusion genes include reverse transcription polymerase chain reaction (RT-PCR) with a detection limit of 1 fmol at urinary condition. RT-PCR is time consuming, costly, and inapplicable for multiplexing. Ability to identify multiple fusion genes in a single sample has become important for diagnostic and clinical purposes. There is a need for a sensitive diagnostic test to detect multiple TMPRSS2–ERG fusion genes for an early diagnosis and prognosis of prostate cancer. Here, we propose to develop an assay for prostate cancer diagnosis using oligonucleotide-functionalized quantum dot and magnetic microparticle for optical detection of rearranged TMPRSS2–ERG fusion genes at a low concentration in urine. We found that our assay was able to identify three different types of fusion gene with a wide detection range and detection limit of 1 fmol (almost the same level of the RT-PCR result reported). Here, we show detection of multiple TMPRSS2–ERG fusion genes using color-coded oligonucleotides in cell lysate and urine. Keywords: fusion genes, prostate cancer, quantum dots, multiplexed assay, optical detectionLee HKim CLee DPark JHSearson PCLee KHDove Medical PressarticleFusion genesProstate cancerQuantum dotsMultiplexed assayOptical detectionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4397-4407 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fusion genes Prostate cancer Quantum dots Multiplexed assay Optical detection Medicine (General) R5-920
spellingShingle	Fusion genes Prostate cancer Quantum dots Multiplexed assay Optical detection Medicine (General) R5-920 Lee H Kim C Lee D Park JH Searson PC Lee KH Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
description	Hyojin Lee,1,* Chloe Kim,2,* Dongjin Lee,1,3,* Jea Ho Park,1,2 Peter C Searson,2 Kwan Hyi Lee1,3 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea; 2Department of Materials Science and Engineering, 3Department of Biomedical Engineering, Korea University of Science and Technology (UST), Daejeon, Republic of Korea *These authors contributed equally to this work Abstract: Recent studies have found that prostate cancer expresses abnormal genetic markers including multiple types of TMPRSS2–ERG fusion genes. The expression level of different TMPRSS2–ERG fusion genes is correlated to pathologic variables of aggressive prostate cancer and disease progression. State-of-the-art methods for detection of TMPRSS2–ERG fusion genes include reverse transcription polymerase chain reaction (RT-PCR) with a detection limit of 1 fmol at urinary condition. RT-PCR is time consuming, costly, and inapplicable for multiplexing. Ability to identify multiple fusion genes in a single sample has become important for diagnostic and clinical purposes. There is a need for a sensitive diagnostic test to detect multiple TMPRSS2–ERG fusion genes for an early diagnosis and prognosis of prostate cancer. Here, we propose to develop an assay for prostate cancer diagnosis using oligonucleotide-functionalized quantum dot and magnetic microparticle for optical detection of rearranged TMPRSS2–ERG fusion genes at a low concentration in urine. We found that our assay was able to identify three different types of fusion gene with a wide detection range and detection limit of 1 fmol (almost the same level of the RT-PCR result reported). Here, we show detection of multiple TMPRSS2–ERG fusion genes using color-coded oligonucleotides in cell lysate and urine. Keywords: fusion genes, prostate cancer, quantum dots, multiplexed assay, optical detection
format	article
author	Lee H Kim C Lee D Park JH Searson PC Lee KH
author_facet	Lee H Kim C Lee D Park JH Searson PC Lee KH
author_sort	Lee H
title	Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
title_short	Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
title_full	Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
title_fullStr	Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
title_full_unstemmed	Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
title_sort	optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/81c7ff7a464e400f84ff47c643758a22
work_keys_str_mv	AT leeh opticalcodingoffusiongenesusingmulticolorquantumdotsforprostatecancerdiagnosis AT kimc opticalcodingoffusiongenesusingmulticolorquantumdotsforprostatecancerdiagnosis AT leed opticalcodingoffusiongenesusingmulticolorquantumdotsforprostatecancerdiagnosis AT parkjh opticalcodingoffusiongenesusingmulticolorquantumdotsforprostatecancerdiagnosis AT searsonpc opticalcodingoffusiongenesusingmulticolorquantumdotsforprostatecancerdiagnosis AT leekh opticalcodingoffusiongenesusingmulticolorquantumdotsforprostatecancerdiagnosis
_version_	1718401016348540928

Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis

Ejemplares similares