Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10

Abstract Background This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. Methods I...

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Autores principales:	Weiyuan Li, Ji-Ao Ma, Xun Sheng, Chunjie Xiao
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Ovarian cancer Chemokines Angiogenesis Immune infiltration Bioinformatics Surgery RD1-811 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/81c8d24183da4872ab79905e7b8ef450
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spelling	oai:doaj.org-article:81c8d24183da4872ab79905e7b8ef4502021-11-21T12:33:36ZScreening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL1010.1186/s12957-021-02440-x1477-7819https://doaj.org/article/81c8d24183da4872ab79905e7b8ef4502021-11-01T00:00:00Zhttps://doi.org/10.1186/s12957-021-02440-xhttps://doaj.org/toc/1477-7819Abstract Background This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. Methods In this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors. Results We found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 were significantly upregulated in OC samples compared with normal tissues. Our data showed that there was a relationship between the expression of CXC chemokines and the infiltration of six types of immune cells significant correlation. In vitro assay confirmed that overexpression of CXCL10 could enhance the killing effect of T cells and inhibit angiogenesis. Further in vivo assay had shown that CXCL10 could affect the progression of ovarian cancer by increasing the expression of cytotoxic T cells and inhibiting angiogenesis. Conclusion In conclusion, we hope that our data will provide new insights into the development of immunotherapy and the selection of prognostic markers for patients with OC.Weiyuan LiJi-Ao MaXun ShengChunjie XiaoBMCarticleOvarian cancerChemokinesAngiogenesisImmune infiltrationBioinformaticsSurgeryRD1-811Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENWorld Journal of Surgical Oncology, Vol 19, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ovarian cancer Chemokines Angiogenesis Immune infiltration Bioinformatics Surgery RD1-811 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Ovarian cancer Chemokines Angiogenesis Immune infiltration Bioinformatics Surgery RD1-811 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Weiyuan Li Ji-Ao Ma Xun Sheng Chunjie Xiao Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
description	Abstract Background This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. Methods In this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors. Results We found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 were significantly upregulated in OC samples compared with normal tissues. Our data showed that there was a relationship between the expression of CXC chemokines and the infiltration of six types of immune cells significant correlation. In vitro assay confirmed that overexpression of CXCL10 could enhance the killing effect of T cells and inhibit angiogenesis. Further in vivo assay had shown that CXCL10 could affect the progression of ovarian cancer by increasing the expression of cytotoxic T cells and inhibiting angiogenesis. Conclusion In conclusion, we hope that our data will provide new insights into the development of immunotherapy and the selection of prognostic markers for patients with OC.
format	article
author	Weiyuan Li Ji-Ao Ma Xun Sheng Chunjie Xiao
author_facet	Weiyuan Li Ji-Ao Ma Xun Sheng Chunjie Xiao
author_sort	Weiyuan Li
title	Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
title_short	Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
title_full	Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
title_fullStr	Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
title_full_unstemmed	Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
title_sort	screening of cxc chemokines in the microenvironment of ovarian cancer and the biological function of cxcl10
publisher	BMC
publishDate	2021
url	https://doaj.org/article/81c8d24183da4872ab79905e7b8ef450
work_keys_str_mv	AT weiyuanli screeningofcxcchemokinesinthemicroenvironmentofovariancancerandthebiologicalfunctionofcxcl10 AT jiaoma screeningofcxcchemokinesinthemicroenvironmentofovariancancerandthebiologicalfunctionofcxcl10 AT xunsheng screeningofcxcchemokinesinthemicroenvironmentofovariancancerandthebiologicalfunctionofcxcl10 AT chunjiexiao screeningofcxcchemokinesinthemicroenvironmentofovariancancerandthebiologicalfunctionofcxcl10
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Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10

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