Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies

Abstract In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform...

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Autores principales:	Martina Bordini, Martina Zappaterra, Francesca Soglia, Massimiliano Petracci, Roberta Davoli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81c91aa39c164ac790e3f9b19324e9b8
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spelling	oai:doaj.org-article:81c91aa39c164ac790e3f9b19324e9b82021-12-02T13:51:05ZWeighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies10.1038/s41598-021-81303-72045-2322https://doaj.org/article/81c91aa39c164ac790e3f9b19324e9b82021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81303-7https://doaj.org/toc/2045-2322Abstract In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weighted Gene Co-expression Network Analysis (WGCNA) using the gene expression profile and meat quality parameters of Pectoralis major muscles analysed in our previous study. Among the 212 modules identified by WGCNA, the red, darkred, midnightblue and paleturquoise4 modules were chosen for subsequent analysis. Functional analysis evidenced pathways involved in extracellular matrix (ECM) organization, collagen metabolism, cellular signaling and unfolded protein response. The hub gene analysis showed several genes coding for ECM components as the most interconnected nodes in the gene network (e.g. COL4A1, COL4A2, LAMA2, LAMA4, FBLN5 and FBN1). In this regard, this study suggests that alterations in ECM composition could somehow activate the cascade of biological reactions that result in the growth-related myopathies onset, and the involvement of Collagen IV alterations in activating the endoplasmic reticulum (ER) stress response may be hypothesized. Therefore, our findings provide further and innovative knowledge concerning the molecular mechanisms related to the breast abnormalities occurrence in modern broilers.Martina BordiniMartina ZappaterraFrancesca SogliaMassimiliano PetracciRoberta DavoliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martina Bordini Martina Zappaterra Francesca Soglia Massimiliano Petracci Roberta Davoli Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
description	Abstract In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weighted Gene Co-expression Network Analysis (WGCNA) using the gene expression profile and meat quality parameters of Pectoralis major muscles analysed in our previous study. Among the 212 modules identified by WGCNA, the red, darkred, midnightblue and paleturquoise4 modules were chosen for subsequent analysis. Functional analysis evidenced pathways involved in extracellular matrix (ECM) organization, collagen metabolism, cellular signaling and unfolded protein response. The hub gene analysis showed several genes coding for ECM components as the most interconnected nodes in the gene network (e.g. COL4A1, COL4A2, LAMA2, LAMA4, FBLN5 and FBN1). In this regard, this study suggests that alterations in ECM composition could somehow activate the cascade of biological reactions that result in the growth-related myopathies onset, and the involvement of Collagen IV alterations in activating the endoplasmic reticulum (ER) stress response may be hypothesized. Therefore, our findings provide further and innovative knowledge concerning the molecular mechanisms related to the breast abnormalities occurrence in modern broilers.
format	article
author	Martina Bordini Martina Zappaterra Francesca Soglia Massimiliano Petracci Roberta Davoli
author_facet	Martina Bordini Martina Zappaterra Francesca Soglia Massimiliano Petracci Roberta Davoli
author_sort	Martina Bordini
title	Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_short	Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_full	Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_fullStr	Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_full_unstemmed	Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies
title_sort	weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler white striping and wooden breast myopathies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/81c91aa39c164ac790e3f9b19324e9b8
work_keys_str_mv	AT martinabordini weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies AT martinazappaterra weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies AT francescasoglia weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies AT massimilianopetracci weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies AT robertadavoli weightedgenecoexpressionnetworkanalysisidentifiesmolecularpathwaysandhubgenesinvolvedinbroilerwhitestripingandwoodenbreastmyopathies
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Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies

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