Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis

Background: Differentiation of psoriasis from non-psoriasis psoriasiform dermatitis (NPPD) may be difficult for dermatopathologists, as lack of distinctive histopathological features in a subset of cases may cause confusion in diagnosis. Objective: As the prototype of psoriasiform dermatitis, ps...

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Autores principales:	Engin Sezer, Almut Böer-Auer, Emel Cetin, Fatma Tokat, Emel Durmaz, Sedef Sahin, Umit Ince
Formato:	article
Lenguaje:	EN
Publicado:	Mattioli1885 2015
Materias:	psoriasis psoriasiform dermatitis proliferation Ki-67 Cyclin D1 Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/81cbe6b0d7e54018b3bb46f8c9a95b25
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spelling	oai:doaj.org-article:81cbe6b0d7e54018b3bb46f8c9a95b252021-11-17T08:31:35ZDiagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis10.5826/dpc.0503a022160-9381https://doaj.org/article/81cbe6b0d7e54018b3bb46f8c9a95b252015-07-01T00:00:00Zhttp://dpcj.org/index.php/dpc/article/view/724https://doaj.org/toc/2160-9381 Background: Differentiation of psoriasis from non-psoriasis psoriasiform dermatitis (NPPD) may be difficult for dermatopathologists, as lack of distinctive histopathological features in a subset of cases may cause confusion in diagnosis. Objective: As the prototype of psoriasiform dermatitis, psoriasis is a hyperproliferative skin disorder with increased epidermal turnover compared with NPPD, we investigated the role of proliferation markers, Ki-67 and Cyclin D1 as diagnostic tools to differentiate psoriasis from other psoriasiform dermatitis. Methods: Histopathological specimens of psoriasis (n = 35) and NPPD (n = 36, 14 pityriasis rubra pilaris, 12 pityriasis rosea and 10 lichen simplex) cases were reviewed and immunohistochemically stained for Ki-67 and Cyclin D1. Ki-67 and Cyclin D1 positive cells were counted for suprabasal, and total epidermal immunostaining per mm2. Results: Suprabasal and total epidermal cell counts for Ki-67 were found to be significantly higher in the psoriasis group compared with the NPPD group (p < 0.05). An important and interesting feature was the presence of a cut-off value for the suprabasal/total epidermal cell count ratio of 75% for Ki-67 immunostaining, which was higher in all patients having psoriasis (range, 77.1% – 92.4%) and lower in all NPPD cases (range, 21.0% – 73.3%). However, suprabasal Cyclin D1 cell counts were higher in the psoriasis group compared with the NPPD group (p < 0.05), total epidermal Cyclin D1 cell counts were not statistically significant in either group (p = 0.167), and a cut-off value for suprabasal/total epidermal cell count ratio to distinguish these two entities was not detected using this immunostain. Conclusions: We suggest that Ki-67 is a more sensitive marker than Cyclin D1 in terms of having a cut-off value of 75% for the suprabasal/total epidermal immunoreactive cell count ratio, which we believe could be useful for dermatopathologists in differentiating psoriasis from other psoriasiform dermatitis. Engin SezerAlmut Böer-AuerEmel CetinFatma TokatEmel DurmazSedef SahinUmit InceMattioli1885articlepsoriasispsoriasiform dermatitisproliferationKi-67Cyclin D1DermatologyRL1-803ENDermatology Practical & Conceptual (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	psoriasis psoriasiform dermatitis proliferation Ki-67 Cyclin D1 Dermatology RL1-803
spellingShingle	psoriasis psoriasiform dermatitis proliferation Ki-67 Cyclin D1 Dermatology RL1-803 Engin Sezer Almut Böer-Auer Emel Cetin Fatma Tokat Emel Durmaz Sedef Sahin Umit Ince Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
description	Background: Differentiation of psoriasis from non-psoriasis psoriasiform dermatitis (NPPD) may be difficult for dermatopathologists, as lack of distinctive histopathological features in a subset of cases may cause confusion in diagnosis. Objective: As the prototype of psoriasiform dermatitis, psoriasis is a hyperproliferative skin disorder with increased epidermal turnover compared with NPPD, we investigated the role of proliferation markers, Ki-67 and Cyclin D1 as diagnostic tools to differentiate psoriasis from other psoriasiform dermatitis. Methods: Histopathological specimens of psoriasis (n = 35) and NPPD (n = 36, 14 pityriasis rubra pilaris, 12 pityriasis rosea and 10 lichen simplex) cases were reviewed and immunohistochemically stained for Ki-67 and Cyclin D1. Ki-67 and Cyclin D1 positive cells were counted for suprabasal, and total epidermal immunostaining per mm2. Results: Suprabasal and total epidermal cell counts for Ki-67 were found to be significantly higher in the psoriasis group compared with the NPPD group (p < 0.05). An important and interesting feature was the presence of a cut-off value for the suprabasal/total epidermal cell count ratio of 75% for Ki-67 immunostaining, which was higher in all patients having psoriasis (range, 77.1% – 92.4%) and lower in all NPPD cases (range, 21.0% – 73.3%). However, suprabasal Cyclin D1 cell counts were higher in the psoriasis group compared with the NPPD group (p < 0.05), total epidermal Cyclin D1 cell counts were not statistically significant in either group (p = 0.167), and a cut-off value for suprabasal/total epidermal cell count ratio to distinguish these two entities was not detected using this immunostain. Conclusions: We suggest that Ki-67 is a more sensitive marker than Cyclin D1 in terms of having a cut-off value of 75% for the suprabasal/total epidermal immunoreactive cell count ratio, which we believe could be useful for dermatopathologists in differentiating psoriasis from other psoriasiform dermatitis.
format	article
author	Engin Sezer Almut Böer-Auer Emel Cetin Fatma Tokat Emel Durmaz Sedef Sahin Umit Ince
author_facet	Engin Sezer Almut Böer-Auer Emel Cetin Fatma Tokat Emel Durmaz Sedef Sahin Umit Ince
author_sort	Engin Sezer
title	Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
title_short	Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
title_full	Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
title_fullStr	Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
title_full_unstemmed	Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
title_sort	diagnostic utility of ki-67 and cyclin d1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis
publisher	Mattioli1885
publishDate	2015
url	https://doaj.org/article/81cbe6b0d7e54018b3bb46f8c9a95b25
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Diagnostic utility of Ki-67 and Cyclin D1 immunostaining in differentiation of psoriasis vs. other psoriasiform dermatitis

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