Poles Apart: Where and How Cells Construct Nisin

ABSTRACT Nisin is a 34-amino-acid lantibiotic that has been used commercially for almost a century as a food preservative. In order to produce active nisin, Lactococcus lactis requires an 11-gene operon that encodes proteins involved in modification, processing, transport, immunity, and regulation....

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Autor principal: Colin Hill
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Publicado: American Society for Microbiology 2020
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Acceso en línea:https://doaj.org/article/81d5e85b0b524ead914819b61cf1d93b
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spelling oai:doaj.org-article:81d5e85b0b524ead914819b61cf1d93b2021-11-15T15:55:44ZPoles Apart: Where and How Cells Construct Nisin10.1128/mBio.02991-202150-7511https://doaj.org/article/81d5e85b0b524ead914819b61cf1d93b2020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02991-20https://doaj.org/toc/2150-7511ABSTRACT Nisin is a 34-amino-acid lantibiotic that has been used commercially for almost a century as a food preservative. In order to produce active nisin, Lactococcus lactis requires an 11-gene operon that encodes proteins involved in modification, processing, transport, immunity, and regulation. While the role of each of the 11 proteins is well understood, the location and spatial organization of the biosynthetic machinery that involves NisA, NisB, NisC, NisT, and NisP remain to be determined. In this elegant paper (J. Chen, A. J. van Heel, and O. P. Kuipers, mBio 11:e02825-20, 2020, https://doi.org/10.1128/mBio.02825-20), we learn that a NisB dimer is recruited to the “old” pole of a dividing cell, where it assembles with NisC to form a modification complex that can engage with NisA. Unexpectedly, the NisT transporter does not stably assemble into this complex but is distributed around the membrane until it engages with the NisABC complex to transport NisA across the membrane, whereupon it dissociates from NisBC.Colin HillAmerican Society for MicrobiologyarticlebacteriocinsbiosynthesisnisinMicrobiologyQR1-502ENmBio, Vol 11, Iss 6 (2020)
institution DOAJ
collection DOAJ
language EN
topic bacteriocins
biosynthesis
nisin
Microbiology
QR1-502
spellingShingle bacteriocins
biosynthesis
nisin
Microbiology
QR1-502
Colin Hill
Poles Apart: Where and How Cells Construct Nisin
description ABSTRACT Nisin is a 34-amino-acid lantibiotic that has been used commercially for almost a century as a food preservative. In order to produce active nisin, Lactococcus lactis requires an 11-gene operon that encodes proteins involved in modification, processing, transport, immunity, and regulation. While the role of each of the 11 proteins is well understood, the location and spatial organization of the biosynthetic machinery that involves NisA, NisB, NisC, NisT, and NisP remain to be determined. In this elegant paper (J. Chen, A. J. van Heel, and O. P. Kuipers, mBio 11:e02825-20, 2020, https://doi.org/10.1128/mBio.02825-20), we learn that a NisB dimer is recruited to the “old” pole of a dividing cell, where it assembles with NisC to form a modification complex that can engage with NisA. Unexpectedly, the NisT transporter does not stably assemble into this complex but is distributed around the membrane until it engages with the NisABC complex to transport NisA across the membrane, whereupon it dissociates from NisBC.
format article
author Colin Hill
author_facet Colin Hill
author_sort Colin Hill
title Poles Apart: Where and How Cells Construct Nisin
title_short Poles Apart: Where and How Cells Construct Nisin
title_full Poles Apart: Where and How Cells Construct Nisin
title_fullStr Poles Apart: Where and How Cells Construct Nisin
title_full_unstemmed Poles Apart: Where and How Cells Construct Nisin
title_sort poles apart: where and how cells construct nisin
publisher American Society for Microbiology
publishDate 2020
url https://doaj.org/article/81d5e85b0b524ead914819b61cf1d93b
work_keys_str_mv AT colinhill polesapartwhereandhowcellsconstructnisin
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