Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1

A gene signature was previously found to be correlated with mosaic adenovirus 26 vaccine protection in simian immunodeficiency virus and simian-human immunodeficiency virus challenge models in non-human primates. In this report, we investigated the presence of this signature as a correlate of reduce...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shida Shangguan, Philip K Ehrenberg, Aviva Geretz, Lauren Yum, Gautam Kundu, Kelly May, Slim Fourati, Krystelle Nganou-Makamdop, LaTonya D Williams, Sheetal Sawant, Eric Lewitus, Punnee Pitisuttithum, Sorachai Nitayaphan, Suwat Chariyalertsak, Supachai Rerks-Ngarm, Morgane Rolland, Daniel C Douek, Peter Gilbert, Georgia D Tomaras, Nelson L Michael, Sandhya Vasan, Rasmi Thomas
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	HIV vaccine transcriptomics single cell CITE-seq vaccine efficacy ADCP Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/81dfbbada87149d49aa3e55c7de54154
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:81dfbbada87149d49aa3e55c7de54154
record_format	dspace
spelling	oai:doaj.org-article:81dfbbada87149d49aa3e55c7de541542021-11-29T12:14:20ZMonocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-110.7554/eLife.695772050-084Xe69577https://doaj.org/article/81dfbbada87149d49aa3e55c7de541542021-09-01T00:00:00Zhttps://elifesciences.org/articles/69577https://doaj.org/toc/2050-084XA gene signature was previously found to be correlated with mosaic adenovirus 26 vaccine protection in simian immunodeficiency virus and simian-human immunodeficiency virus challenge models in non-human primates. In this report, we investigated the presence of this signature as a correlate of reduced risk in human clinical trials and potential mechanisms of protection. The absence of this gene signature in the DNA/rAd5 human vaccine trial, which did not show efficacy, strengthens our hypothesis that this signature is only enriched in studies that demonstrated protection. This gene signature was enriched in the partially effective RV144 human trial that administered the ALVAC/protein vaccine, and we find that the signature associates with both decreased risk of HIV-1 acquisition and increased vaccine efficacy (VE). Total RNA-seq in a clinical trial that used the same vaccine regimen as the RV144 HIV vaccine implicated antibody-dependent cellular phagocytosis (ADCP) as a potential mechanism of vaccine protection. CITE-seq profiling of 53 surface markers and transcriptomes of 53,777 single cells from the same trial showed that genes in this signature were primarily expressed in cells belonging to the myeloid lineage, including monocytes, which are major effector cells for ADCP. The consistent association of this transcriptome signature with VE represents a tool both to identify potential mechanisms, as with ADCP here, and to screen novel approaches to accelerate the development of new vaccine candidates.Shida ShangguanPhilip K EhrenbergAviva GeretzLauren YumGautam KunduKelly MaySlim FouratiKrystelle Nganou-MakamdopLaTonya D WilliamsSheetal SawantEric LewitusPunnee PitisuttithumSorachai NitayaphanSuwat ChariyalertsakSupachai Rerks-NgarmMorgane RollandDaniel C DouekPeter GilbertGeorgia D TomarasNelson L MichaelSandhya VasanRasmi ThomaseLife Sciences Publications LtdarticleHIV vaccinetranscriptomicssingle cellCITE-seqvaccine efficacyADCPMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HIV vaccine transcriptomics single cell CITE-seq vaccine efficacy ADCP Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	HIV vaccine transcriptomics single cell CITE-seq vaccine efficacy ADCP Medicine R Science Q Biology (General) QH301-705.5 Shida Shangguan Philip K Ehrenberg Aviva Geretz Lauren Yum Gautam Kundu Kelly May Slim Fourati Krystelle Nganou-Makamdop LaTonya D Williams Sheetal Sawant Eric Lewitus Punnee Pitisuttithum Sorachai Nitayaphan Suwat Chariyalertsak Supachai Rerks-Ngarm Morgane Rolland Daniel C Douek Peter Gilbert Georgia D Tomaras Nelson L Michael Sandhya Vasan Rasmi Thomas Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1
description	A gene signature was previously found to be correlated with mosaic adenovirus 26 vaccine protection in simian immunodeficiency virus and simian-human immunodeficiency virus challenge models in non-human primates. In this report, we investigated the presence of this signature as a correlate of reduced risk in human clinical trials and potential mechanisms of protection. The absence of this gene signature in the DNA/rAd5 human vaccine trial, which did not show efficacy, strengthens our hypothesis that this signature is only enriched in studies that demonstrated protection. This gene signature was enriched in the partially effective RV144 human trial that administered the ALVAC/protein vaccine, and we find that the signature associates with both decreased risk of HIV-1 acquisition and increased vaccine efficacy (VE). Total RNA-seq in a clinical trial that used the same vaccine regimen as the RV144 HIV vaccine implicated antibody-dependent cellular phagocytosis (ADCP) as a potential mechanism of vaccine protection. CITE-seq profiling of 53 surface markers and transcriptomes of 53,777 single cells from the same trial showed that genes in this signature were primarily expressed in cells belonging to the myeloid lineage, including monocytes, which are major effector cells for ADCP. The consistent association of this transcriptome signature with VE represents a tool both to identify potential mechanisms, as with ADCP here, and to screen novel approaches to accelerate the development of new vaccine candidates.
format	article
author	Shida Shangguan Philip K Ehrenberg Aviva Geretz Lauren Yum Gautam Kundu Kelly May Slim Fourati Krystelle Nganou-Makamdop LaTonya D Williams Sheetal Sawant Eric Lewitus Punnee Pitisuttithum Sorachai Nitayaphan Suwat Chariyalertsak Supachai Rerks-Ngarm Morgane Rolland Daniel C Douek Peter Gilbert Georgia D Tomaras Nelson L Michael Sandhya Vasan Rasmi Thomas
author_facet	Shida Shangguan Philip K Ehrenberg Aviva Geretz Lauren Yum Gautam Kundu Kelly May Slim Fourati Krystelle Nganou-Makamdop LaTonya D Williams Sheetal Sawant Eric Lewitus Punnee Pitisuttithum Sorachai Nitayaphan Suwat Chariyalertsak Supachai Rerks-Ngarm Morgane Rolland Daniel C Douek Peter Gilbert Georgia D Tomaras Nelson L Michael Sandhya Vasan Rasmi Thomas
author_sort	Shida Shangguan
title	Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1
title_short	Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1
title_full	Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1
title_fullStr	Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1
title_full_unstemmed	Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1
title_sort	monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against hiv-1
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/81dfbbada87149d49aa3e55c7de54154
work_keys_str_mv	AT shidashangguan monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT philipkehrenberg monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT avivageretz monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT laurenyum monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT gautamkundu monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT kellymay monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT slimfourati monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT krystellenganoumakamdop monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT latonyadwilliams monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT sheetalsawant monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT ericlewitus monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT punneepitisuttithum monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT sorachainitayaphan monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT suwatchariyalertsak monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT supachairerksngarm monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT morganerolland monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT danielcdouek monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT petergilbert monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT georgiadtomaras monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT nelsonlmichael monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT sandhyavasan monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1 AT rasmithomas monocytederivedtranscriptomesignatureindicatesantibodydependentcellularphagocytosisasapotentialmechanismofvaccineinducedprotectionagainsthiv1
_version_	1718407412197621760

Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1

Ejemplares similares