Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections

Barbara Strzalka-Mrozik,1 Malgorzata Kimsa-Furdzik,2 Adam Kabiesz,3 Katarzyna Michalska-Malecka,3,4 Malgorzata Nita,5 Urszula Mazurek1 1Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland; 2Depart...

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Autores principales:	Strzalka-Mrozik B, Kimsa-Furdzik M, Kabiesz A, Michalska-Malecka K, Nita M, Mazurek U
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Age-related macular degeneration IGF family ranibizumab mRNA Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/81e024da6192425cbf61760cc62f8928
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spelling	oai:doaj.org-article:81e024da6192425cbf61760cc62f89282021-12-02T03:26:23ZGene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections1178-1998https://doaj.org/article/81e024da6192425cbf61760cc62f89282017-09-01T00:00:00Zhttps://www.dovepress.com/gene-expression-levels-of-the-insulin-like-growth-factor-family-in-pat-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Barbara Strzalka-Mrozik,1 Malgorzata Kimsa-Furdzik,2 Adam Kabiesz,3 Katarzyna Michalska-Malecka,3,4 Malgorzata Nita,5 Urszula Mazurek1 1Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland; 2Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; 3University Center for Ophthalmology and Oncology, Independent Public Clinical Hospital, Medical University of Silesia, Katowice, Poland; 4Department of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; 5Domestic and Specialized Medicine Centre “Dilmed”, Katowice, Poland Purpose: The present study focused on the assessment of the mRNA levels of the insulin-like growth factor (IGF) family in patients with the exudative form of age-related macular degeneration (AMD) before and after ranibizumab intravitreal injections.Patients and methods: An analysis of the expression profile of the IGF family of genes in patients with AMD was carried out using the oligonucleotide microarray and quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) methods.Results: In the peripheral blood mononuclear cells (PBMCs) obtained from AMD group receiving ranibizumab compared to the peripheral blood mononuclear cells from AMD group before ranibizumab treatment using oligonucleotide microarray technique, six statistically significant differentially expressed transcripts related to the IGF family were detected (unpaired t-test, p<0.05, fold change >1.5). Moreover, analysis using the real-time RT-qPCR technique revealed statistically significant differences in the IGF2 and IGF2R mRNA levels (Mann–Whitney U test, p<0.05) between the two groups that were studied. Statistical analyses of both oligonucleotide microarray and real-time RT-qPCR results demonstrated a significant decreased expression only for IGF2 mRNA.Conclusion: Our results revealed a changed expression of IGF2 mRNA after ranibizumab treatment. Keywords: age-related macular degeneration, IGF family, ranibizumab, mRNAStrzalka-Mrozik BKimsa-Furdzik MKabiesz AMichalska-Malecka KNita MMazurek UDove Medical PressarticleAge-related macular degenerationIGF familyranibizumabmRNAGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 12, Pp 1401-1408 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Age-related macular degeneration IGF family ranibizumab mRNA Geriatrics RC952-954.6
spellingShingle	Age-related macular degeneration IGF family ranibizumab mRNA Geriatrics RC952-954.6 Strzalka-Mrozik B Kimsa-Furdzik M Kabiesz A Michalska-Malecka K Nita M Mazurek U Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
description	Barbara Strzalka-Mrozik,1 Malgorzata Kimsa-Furdzik,2 Adam Kabiesz,3 Katarzyna Michalska-Malecka,3,4 Malgorzata Nita,5 Urszula Mazurek1 1Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland; 2Department of Biochemistry, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; 3University Center for Ophthalmology and Oncology, Independent Public Clinical Hospital, Medical University of Silesia, Katowice, Poland; 4Department of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland; 5Domestic and Specialized Medicine Centre “Dilmed”, Katowice, Poland Purpose: The present study focused on the assessment of the mRNA levels of the insulin-like growth factor (IGF) family in patients with the exudative form of age-related macular degeneration (AMD) before and after ranibizumab intravitreal injections.Patients and methods: An analysis of the expression profile of the IGF family of genes in patients with AMD was carried out using the oligonucleotide microarray and quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) methods.Results: In the peripheral blood mononuclear cells (PBMCs) obtained from AMD group receiving ranibizumab compared to the peripheral blood mononuclear cells from AMD group before ranibizumab treatment using oligonucleotide microarray technique, six statistically significant differentially expressed transcripts related to the IGF family were detected (unpaired t-test, p<0.05, fold change >1.5). Moreover, analysis using the real-time RT-qPCR technique revealed statistically significant differences in the IGF2 and IGF2R mRNA levels (Mann–Whitney U test, p<0.05) between the two groups that were studied. Statistical analyses of both oligonucleotide microarray and real-time RT-qPCR results demonstrated a significant decreased expression only for IGF2 mRNA.Conclusion: Our results revealed a changed expression of IGF2 mRNA after ranibizumab treatment. Keywords: age-related macular degeneration, IGF family, ranibizumab, mRNA
format	article
author	Strzalka-Mrozik B Kimsa-Furdzik M Kabiesz A Michalska-Malecka K Nita M Mazurek U
author_facet	Strzalka-Mrozik B Kimsa-Furdzik M Kabiesz A Michalska-Malecka K Nita M Mazurek U
author_sort	Strzalka-Mrozik B
title	Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
title_short	Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
title_full	Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
title_fullStr	Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
title_full_unstemmed	Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections
title_sort	gene expression levels of the insulin-like growth factor family in patients with amd before and after ranibizumab intravitreal injections
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/81e024da6192425cbf61760cc62f8928
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Gene expression levels of the insulin-like growth factor family in patients with AMD before and after ranibizumab intravitreal injections

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