Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.

During replication, RNA viruses accumulate genome alterations, such as mutations and deletions. The interactions between individual variants can determine the fitness of the virus population and, thus, the outcome of infection. To investigate the effects of defective interfering genomes (DI) on wild...

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Autores principales:	Yuta Shirogane, Elsa Rousseau, Jakub Voznica, Yinghong Xiao, Weiheng Su, Adam Catching, Zachary J Whitfield, Igor M Rouzine, Simone Bianco, Raul Andino
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/81e3947dd5f54629a507068079aad99d
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spelling	oai:doaj.org-article:81e3947dd5f54629a507068079aad99d2021-12-02T20:00:06ZExperimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.1553-73661553-737410.1371/journal.ppat.1009277https://doaj.org/article/81e3947dd5f54629a507068079aad99d2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009277https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374During replication, RNA viruses accumulate genome alterations, such as mutations and deletions. The interactions between individual variants can determine the fitness of the virus population and, thus, the outcome of infection. To investigate the effects of defective interfering genomes (DI) on wild-type (WT) poliovirus replication, we developed an ordinary differential equation model, which enables exploring the parameter space of the WT and DI competition. We also experimentally examined virus and DI replication kinetics during co-infection, and used these data to infer model parameters. Our model identifies, and our experimental measurements confirm, that the efficiencies of DI genome replication and encapsidation are two most critical parameters determining the outcome of WT replication. However, an equilibrium can be established which enables WT to replicate, albeit to reduced levels.Yuta ShiroganeElsa RousseauJakub VoznicaYinghong XiaoWeiheng SuAdam CatchingZachary J WhitfieldIgor M RouzineSimone BiancoRaul AndinoPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 9, p e1009277 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Yuta Shirogane Elsa Rousseau Jakub Voznica Yinghong Xiao Weiheng Su Adam Catching Zachary J Whitfield Igor M Rouzine Simone Bianco Raul Andino Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
description	During replication, RNA viruses accumulate genome alterations, such as mutations and deletions. The interactions between individual variants can determine the fitness of the virus population and, thus, the outcome of infection. To investigate the effects of defective interfering genomes (DI) on wild-type (WT) poliovirus replication, we developed an ordinary differential equation model, which enables exploring the parameter space of the WT and DI competition. We also experimentally examined virus and DI replication kinetics during co-infection, and used these data to infer model parameters. Our model identifies, and our experimental measurements confirm, that the efficiencies of DI genome replication and encapsidation are two most critical parameters determining the outcome of WT replication. However, an equilibrium can be established which enables WT to replicate, albeit to reduced levels.
format	article
author	Yuta Shirogane Elsa Rousseau Jakub Voznica Yinghong Xiao Weiheng Su Adam Catching Zachary J Whitfield Igor M Rouzine Simone Bianco Raul Andino
author_facet	Yuta Shirogane Elsa Rousseau Jakub Voznica Yinghong Xiao Weiheng Su Adam Catching Zachary J Whitfield Igor M Rouzine Simone Bianco Raul Andino
author_sort	Yuta Shirogane
title	Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
title_short	Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
title_full	Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
title_fullStr	Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
title_full_unstemmed	Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
title_sort	experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/81e3947dd5f54629a507068079aad99d
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Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome.

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