Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis
The aim of this study was to obtain a Brazilian red propolis (BRP) enriched composite resin and to perform the characterization of its antibacterial activity, mechanical, and physical-chemical properties. Brazilian red propolis ethyl acetate extract (EABRP) was characterized by LC-ESI-Orbitrap-FTMS,...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:81e4a7552698495382410426a44411002021-12-01T13:50:32ZPropolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis1663-981210.3389/fphar.2021.787633https://doaj.org/article/81e4a7552698495382410426a44411002021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.787633/fullhttps://doaj.org/toc/1663-9812The aim of this study was to obtain a Brazilian red propolis (BRP) enriched composite resin and to perform the characterization of its antibacterial activity, mechanical, and physical-chemical properties. Brazilian red propolis ethyl acetate extract (EABRP) was characterized by LC-ESI-Orbitrap-FTMS, UPLC-DAD, antibacterial activity, total flavonoids content, and radical scavenging capacity. BRP was incorporated to a commercial composite resin (RC) to obtain BRP enriched composite at 0.1, 0.15 and 0.25% (RP10, RP15 and RP25, respectively). The antibacterial activity RPs was evaluated against Streptococcus mutans by contact direct test and expressed by antibacterial ratio. The RPs were characterized as its cytotoxicity against 3T3 fibroblasts, flexural strength (FS), Knoop microhardness (KHN), post-cure depth (CD), degree of conversion (DC%), water sorption (Wsp), water solubility (Wsl), average roughness (Ra), and thermal analysis. Were identified 50 chemical compounds from BRP extract by LC-ESI-Orbitrap-FTMS. EABRP was bacteriostatic and bactericide at 125 and 500 μg/ml, respectively. The RP25 exhibited antibacterial ratio of 90.76% after 1 h of direct contact with S. mutans (p < 0.0001) while RC no showed significative antibacterial activity (p = 0.1865), both compared with cell control group. RPs and RC no showed cytotoxicity. RPs exhibited CD from 2.74 to 4.48 mm, DC% from 80.70 to 83.96%, Wsp from 17.15 to 21.67 μg/mm3, Wsl from 3.66 to 4.20 μg/mm3, Ra from 14.48 to 20.76 nm. RPs showed thermal resistance between 448–455°C. The results support that propolis can be used on development of modified composite resins that show antibacterial activity and that have compatible mechanical and physical-chemical properties to the indicate for composite resins.José Marcos dos Santos OliveiraJosé Marcos dos Santos OliveiraThéo Fortes Silveira CavalcantiThéo Fortes Silveira CavalcantiIngrid Ferreira LeiteDávida Maria Ribeiro Cardoso dos SantosIsabel Cristina Celerino de Moraes PortoIsabel Cristina Celerino de Moraes PortoFernanda Lima Torres de AquinoArtur Falqueto SonsinRenata Matos Lamenha LinsRafael Pino VittiJohnnatan Duarte de FreitasEmiliano de Oliveira BarretoSamuel Teixeira de SouzaRegianne Umeko KamiyaTiciano Gomes do NascimentoJosealdo TonholoFrontiers Media S.A.articlepropolisantibacterial activityStreptococcus mutansdental materialsmechanical propertiesfibroblastsTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021) |
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| topic |
propolis antibacterial activity Streptococcus mutans dental materials mechanical properties fibroblasts Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
propolis antibacterial activity Streptococcus mutans dental materials mechanical properties fibroblasts Therapeutics. Pharmacology RM1-950 José Marcos dos Santos Oliveira José Marcos dos Santos Oliveira Théo Fortes Silveira Cavalcanti Théo Fortes Silveira Cavalcanti Ingrid Ferreira Leite Dávida Maria Ribeiro Cardoso dos Santos Isabel Cristina Celerino de Moraes Porto Isabel Cristina Celerino de Moraes Porto Fernanda Lima Torres de Aquino Artur Falqueto Sonsin Renata Matos Lamenha Lins Rafael Pino Vitti Johnnatan Duarte de Freitas Emiliano de Oliveira Barreto Samuel Teixeira de Souza Regianne Umeko Kamiya Ticiano Gomes do Nascimento Josealdo Tonholo Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis |
| description |
The aim of this study was to obtain a Brazilian red propolis (BRP) enriched composite resin and to perform the characterization of its antibacterial activity, mechanical, and physical-chemical properties. Brazilian red propolis ethyl acetate extract (EABRP) was characterized by LC-ESI-Orbitrap-FTMS, UPLC-DAD, antibacterial activity, total flavonoids content, and radical scavenging capacity. BRP was incorporated to a commercial composite resin (RC) to obtain BRP enriched composite at 0.1, 0.15 and 0.25% (RP10, RP15 and RP25, respectively). The antibacterial activity RPs was evaluated against Streptococcus mutans by contact direct test and expressed by antibacterial ratio. The RPs were characterized as its cytotoxicity against 3T3 fibroblasts, flexural strength (FS), Knoop microhardness (KHN), post-cure depth (CD), degree of conversion (DC%), water sorption (Wsp), water solubility (Wsl), average roughness (Ra), and thermal analysis. Were identified 50 chemical compounds from BRP extract by LC-ESI-Orbitrap-FTMS. EABRP was bacteriostatic and bactericide at 125 and 500 μg/ml, respectively. The RP25 exhibited antibacterial ratio of 90.76% after 1 h of direct contact with S. mutans (p < 0.0001) while RC no showed significative antibacterial activity (p = 0.1865), both compared with cell control group. RPs and RC no showed cytotoxicity. RPs exhibited CD from 2.74 to 4.48 mm, DC% from 80.70 to 83.96%, Wsp from 17.15 to 21.67 μg/mm3, Wsl from 3.66 to 4.20 μg/mm3, Ra from 14.48 to 20.76 nm. RPs showed thermal resistance between 448–455°C. The results support that propolis can be used on development of modified composite resins that show antibacterial activity and that have compatible mechanical and physical-chemical properties to the indicate for composite resins. |
| format |
article |
| author |
José Marcos dos Santos Oliveira José Marcos dos Santos Oliveira Théo Fortes Silveira Cavalcanti Théo Fortes Silveira Cavalcanti Ingrid Ferreira Leite Dávida Maria Ribeiro Cardoso dos Santos Isabel Cristina Celerino de Moraes Porto Isabel Cristina Celerino de Moraes Porto Fernanda Lima Torres de Aquino Artur Falqueto Sonsin Renata Matos Lamenha Lins Rafael Pino Vitti Johnnatan Duarte de Freitas Emiliano de Oliveira Barreto Samuel Teixeira de Souza Regianne Umeko Kamiya Ticiano Gomes do Nascimento Josealdo Tonholo |
| author_facet |
José Marcos dos Santos Oliveira José Marcos dos Santos Oliveira Théo Fortes Silveira Cavalcanti Théo Fortes Silveira Cavalcanti Ingrid Ferreira Leite Dávida Maria Ribeiro Cardoso dos Santos Isabel Cristina Celerino de Moraes Porto Isabel Cristina Celerino de Moraes Porto Fernanda Lima Torres de Aquino Artur Falqueto Sonsin Renata Matos Lamenha Lins Rafael Pino Vitti Johnnatan Duarte de Freitas Emiliano de Oliveira Barreto Samuel Teixeira de Souza Regianne Umeko Kamiya Ticiano Gomes do Nascimento Josealdo Tonholo |
| author_sort |
José Marcos dos Santos Oliveira |
| title |
Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis |
| title_short |
Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis |
| title_full |
Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis |
| title_fullStr |
Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis |
| title_full_unstemmed |
Propolis in Oral Healthcare: Antibacterial Activity of a Composite Resin Enriched With Brazilian Red Propolis |
| title_sort |
propolis in oral healthcare: antibacterial activity of a composite resin enriched with brazilian red propolis |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/81e4a7552698495382410426a4441100 |
| work_keys_str_mv |
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