Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse

Shewei Guo,* Yingwei Zhen,* Anran Wang Department of Neurosurgery, The First Affiliated Hospital, Zhengzhou University, Henan, China *These authors contributed equally to this work Abstract: Traumatic brain injury (TBI) has emerged as a leading cause of mortality and morbidity worldwide. Transpla...

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Autores principales:	Guo SW, Zhen YW, Wang AR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	TBI BMSCs angiogenesis neurological function Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/81eef2cd4f5b48a7882b5487cfc15094
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spelling	oai:doaj.org-article:81eef2cd4f5b48a7882b5487cfc150942021-12-02T05:03:25ZTransplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse1178-2021https://doaj.org/article/81eef2cd4f5b48a7882b5487cfc150942017-11-01T00:00:00Zhttps://www.dovepress.com/transplantation-of-bone-mesenchymal-stem-cells-promotes-angiogenesis-a-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Shewei Guo,* Yingwei Zhen,* Anran Wang Department of Neurosurgery, The First Affiliated Hospital, Zhengzhou University, Henan, China *These authors contributed equally to this work Abstract: Traumatic brain injury (TBI) has emerged as a leading cause of mortality and morbidity worldwide. Transplantation of bone mesenchymal stem cells (BMSCs) has emerged as a promising treatment for various central nervous system diseases. This study aims to evaluate the effect of BMSCs transplantation by intravenous injection on neurological function and angiogenesis of the TBI mice. C57BL/6 male mice were randomly divided into four groups: control, sham, TBI, and BMSC. Functional neurological evaluation was performed 1, 4, 7, 14, and 21 days after TBI using neurological severity scores. The impairment of learning and memory in mice was evaluated 14 days after TBI by Morris water maze experiment. Mice were sacrificed 14 days after TBI, and then brain sections were stained by terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling staining to assess brain neuronal apoptosis. Immunohistochemistry was conducted to evaluate caspase-3 activity and identify vascular distribution and microvessel density. Protein and mRNA levels of vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) in brain tissues were analyzed by Western blot and quantitative real-time polymerase chain reaction, respectively. BMSCs transplantation promoted recovery of neurological function, ameliorated impairment of learning and memory, attenuated neuronal apoptosis, and diminished caspase-3 activation in mice after TBI. Also, BMSCs transplantation upregulated expressions of VEGF and Ang-1 and promoted the formation of microvessels in brain tissues after TBI. Our study demonstrated the important role of BMSCs transplantation in TBI mouse and indicated that the underlying mechanism was through promoting angiogenesis and improving neurological function. This provides a novel and effective strategy for cell-based therapy in the treatment of TBI. Keywords: TBI, BMSCs, angiogenesis, neurological function Guo SWZhen YWWang ARDove Medical PressarticleTBIBMSCsangiogenesisneurological functionNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 13, Pp 2757-2765 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	TBI BMSCs angiogenesis neurological function Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	TBI BMSCs angiogenesis neurological function Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Guo SW Zhen YW Wang AR Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
description	Shewei Guo,* Yingwei Zhen,* Anran Wang Department of Neurosurgery, The First Affiliated Hospital, Zhengzhou University, Henan, China *These authors contributed equally to this work Abstract: Traumatic brain injury (TBI) has emerged as a leading cause of mortality and morbidity worldwide. Transplantation of bone mesenchymal stem cells (BMSCs) has emerged as a promising treatment for various central nervous system diseases. This study aims to evaluate the effect of BMSCs transplantation by intravenous injection on neurological function and angiogenesis of the TBI mice. C57BL/6 male mice were randomly divided into four groups: control, sham, TBI, and BMSC. Functional neurological evaluation was performed 1, 4, 7, 14, and 21 days after TBI using neurological severity scores. The impairment of learning and memory in mice was evaluated 14 days after TBI by Morris water maze experiment. Mice were sacrificed 14 days after TBI, and then brain sections were stained by terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling staining to assess brain neuronal apoptosis. Immunohistochemistry was conducted to evaluate caspase-3 activity and identify vascular distribution and microvessel density. Protein and mRNA levels of vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) in brain tissues were analyzed by Western blot and quantitative real-time polymerase chain reaction, respectively. BMSCs transplantation promoted recovery of neurological function, ameliorated impairment of learning and memory, attenuated neuronal apoptosis, and diminished caspase-3 activation in mice after TBI. Also, BMSCs transplantation upregulated expressions of VEGF and Ang-1 and promoted the formation of microvessels in brain tissues after TBI. Our study demonstrated the important role of BMSCs transplantation in TBI mouse and indicated that the underlying mechanism was through promoting angiogenesis and improving neurological function. This provides a novel and effective strategy for cell-based therapy in the treatment of TBI. Keywords: TBI, BMSCs, angiogenesis, neurological function
format	article
author	Guo SW Zhen YW Wang AR
author_facet	Guo SW Zhen YW Wang AR
author_sort	Guo SW
title	Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
title_short	Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
title_full	Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
title_fullStr	Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
title_full_unstemmed	Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
title_sort	transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/81eef2cd4f5b48a7882b5487cfc15094
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_version_	1718400720683663360

Transplantation of bone mesenchymal stem cells promotes angiogenesis and improves neurological function after traumatic brain injury in mouse

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