Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material

Paul M Maschhoff,1 Benjamin M Geilich,2 Thomas J Webster1,31Department of Chemical Engineering, 2Program in Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstrac...

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Autores principales:	Maschhoff PM, Geilich BM, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/81f298b64dbd4163ba85425e14c14474
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spelling	oai:doaj.org-article:81f298b64dbd4163ba85425e14c144742021-12-02T05:10:26ZGreater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material1178-2013https://doaj.org/article/81f298b64dbd4163ba85425e14c144742013-12-01T00:00:00Zhttp://www.dovepress.com/greater-fibroblast-proliferation-on-an-ultrasonicated-znopvc-nanocompo-a15393https://doaj.org/toc/1178-2013 Paul M Maschhoff,1 Benjamin M Geilich,2 Thomas J Webster1,31Department of Chemical Engineering, 2Program in Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: There has been a significant and growing concern over nosocomial medical device infections. Previous studies have demonstrated that embedding nanoparticles alone (specifically, zinc oxide [ZnO]) in conventional polymers (eg, polyvinyl chloride [PVC]) can decrease bacteria growth and may have the potential to prevent or disrupt bacterial processes that lead to infection. However, little to no studies have been conducted to determine mammalian cell functions on such a nanocomposite material. Clearly, for certain medical device applications, maintaining healthy mammalian cell functions while decreasing bacteria growth is imperative (yet uncommon). For this reason, in the presented study, ZnO nanoparticles of varying sizes (from 10 nm to >200 nm in diameter) and functionalization (including no functionalization to doping with aluminum oxide and functionalizing with a silane coupling agent KH550) were incorporated into PVC either with or without ultrasonication. Results of this study provided the first evidence of greater fibroblast density after 18 hours of culture on the smallest ZnO nanoparticle incorporated PVC samples with dispersion aided by ultrasonication. Specifically, the greatest amount of fibroblast proliferation was measured on ZnO nanoparticles functionalized with a silane coupling agent KH550; this sample exhibited the greatest dispersion of ZnO nanoparticles. Water droplet tests showed a general trend of decreased hydrophilicity when adding any of the ZnO nanoparticles to PVC, but an increase in hydrophilicity (albeit still below controls or pure PVC) when using ultrasonication to increase ZnO nanoparticle dispersion. Future studies will have to correlate this change in wettability to initial protein adsorption events that may explain fibroblast behavior. Mechanical tests also provided evidence of the ability to tailor mechanical properties of the ZnO/PVC nanocomposites through the use of the different ZnO nanoparticles. Coupled with previous antibacterial studies, the present study demonstrated that highly dispersed ZnO/PVC nanocomposite materials should be further studied for numerous medical device applications.Keywords: ZnO, nanoparticles, PVC, fibroblast, dispersion, nanotechnologyMaschhoff PMGeilich BMWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 257-263 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Maschhoff PM Geilich BM Webster TJ Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material
description	Paul M Maschhoff,1 Benjamin M Geilich,2 Thomas J Webster1,31Department of Chemical Engineering, 2Program in Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi ArabiaAbstract: There has been a significant and growing concern over nosocomial medical device infections. Previous studies have demonstrated that embedding nanoparticles alone (specifically, zinc oxide [ZnO]) in conventional polymers (eg, polyvinyl chloride [PVC]) can decrease bacteria growth and may have the potential to prevent or disrupt bacterial processes that lead to infection. However, little to no studies have been conducted to determine mammalian cell functions on such a nanocomposite material. Clearly, for certain medical device applications, maintaining healthy mammalian cell functions while decreasing bacteria growth is imperative (yet uncommon). For this reason, in the presented study, ZnO nanoparticles of varying sizes (from 10 nm to >200 nm in diameter) and functionalization (including no functionalization to doping with aluminum oxide and functionalizing with a silane coupling agent KH550) were incorporated into PVC either with or without ultrasonication. Results of this study provided the first evidence of greater fibroblast density after 18 hours of culture on the smallest ZnO nanoparticle incorporated PVC samples with dispersion aided by ultrasonication. Specifically, the greatest amount of fibroblast proliferation was measured on ZnO nanoparticles functionalized with a silane coupling agent KH550; this sample exhibited the greatest dispersion of ZnO nanoparticles. Water droplet tests showed a general trend of decreased hydrophilicity when adding any of the ZnO nanoparticles to PVC, but an increase in hydrophilicity (albeit still below controls or pure PVC) when using ultrasonication to increase ZnO nanoparticle dispersion. Future studies will have to correlate this change in wettability to initial protein adsorption events that may explain fibroblast behavior. Mechanical tests also provided evidence of the ability to tailor mechanical properties of the ZnO/PVC nanocomposites through the use of the different ZnO nanoparticles. Coupled with previous antibacterial studies, the present study demonstrated that highly dispersed ZnO/PVC nanocomposite materials should be further studied for numerous medical device applications.Keywords: ZnO, nanoparticles, PVC, fibroblast, dispersion, nanotechnology
format	article
author	Maschhoff PM Geilich BM Webster TJ
author_facet	Maschhoff PM Geilich BM Webster TJ
author_sort	Maschhoff PM
title	Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material
title_short	Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material
title_full	Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material
title_fullStr	Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material
title_full_unstemmed	Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material
title_sort	greater fibroblast proliferation on an ultrasonicated zno/pvc nanocomposite material
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/81f298b64dbd4163ba85425e14c14474
work_keys_str_mv	AT maschhoffpm greaterfibroblastproliferationonanultrasonicatedznopvcnanocompositematerial AT geilichbm greaterfibroblastproliferationonanultrasonicatedznopvcnanocompositematerial AT webstertj greaterfibroblastproliferationonanultrasonicatedznopvcnanocompositematerial
_version_	1718400539799060480

Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material

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