Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities

Abstract Phaeocystis pouchetii (Hariot) Lagerheim, 1893 regularly dominates phytoplankton blooms in higher latitudes spanning from the English Channel to the Arctic. Through zooplankton grazing and microbial activity, it is considered to be a key resource for the entire marine food web, but the actu...

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Autores principales:	Constanze Kuhlisch, Julia Althammer, Andrey F. Sazhin, Hans H. Jakobsen, Jens C. Nejstgaard, Georg Pohnert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81f5e761d1294361badc66a4f9b08250
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spelling	oai:doaj.org-article:81f5e761d1294361badc66a4f9b082502021-12-02T12:33:52ZMetabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities10.1038/s41598-020-77169-w2045-2322https://doaj.org/article/81f5e761d1294361badc66a4f9b082502020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77169-whttps://doaj.org/toc/2045-2322Abstract Phaeocystis pouchetii (Hariot) Lagerheim, 1893 regularly dominates phytoplankton blooms in higher latitudes spanning from the English Channel to the Arctic. Through zooplankton grazing and microbial activity, it is considered to be a key resource for the entire marine food web, but the actual relevance of biomass transfer to higher trophic levels is still under discussion. Cell physiology and algal nutritional state are suggested to be major factors controlling the observed variability in zooplankton grazing. However, no data have so far yielded insights into the metabolic state of Phaeocystis populations that would allow testing this hypothesis. Therefore, endometabolic markers of different growth phases were determined in laboratory batch cultures using comparative metabolomics and quantified in different phytoplankton blooms in the field. Metabolites, produced during exponential, early and late stationary growth of P. pouchetii, were profiled using gas chromatography-mass spectrometry. Then, metabolites were characterized that correlate with the growth phases using multivariate statistical analysis. Free amino acids characterized the exponential growth, whereas the early stationary phase was correlated with sugar alcohols, mono- and disaccharides. In the late stationary phase, free fatty acids, sterols and terpenes increased. These marker metabolites were then traced in Phaeocystis blooms during a cruise in the Barents Sea and North Norwegian fjords. About 50 endometabolites of P. pouchetii were detected in natural phytoplankton communities. Mannitol, scyllo-inositol, 24-methylcholesta-5,22-dien-3β-ol, and several free fatty acids were characteristic for Phaeocystis-dominated blooms but showed variability between them. Distinct metabolic profiles were detected in the nutrient-depleted community in the inner Porsangerfjord (< 0.5 µM NO3 −, < 0.1 µM PO 4 3− ), with high relative amounts of free mono- and disaccharides indicative for a limited culture. This study thereby shows how the variable physiology of phytoplankton can alter the metabolic landscape of entire plankton communities.Constanze KuhlischJulia AlthammerAndrey F. SazhinHans H. JakobsenJens C. NejstgaardGeorg PohnertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Constanze Kuhlisch Julia Althammer Andrey F. Sazhin Hans H. Jakobsen Jens C. Nejstgaard Georg Pohnert Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities
description	Abstract Phaeocystis pouchetii (Hariot) Lagerheim, 1893 regularly dominates phytoplankton blooms in higher latitudes spanning from the English Channel to the Arctic. Through zooplankton grazing and microbial activity, it is considered to be a key resource for the entire marine food web, but the actual relevance of biomass transfer to higher trophic levels is still under discussion. Cell physiology and algal nutritional state are suggested to be major factors controlling the observed variability in zooplankton grazing. However, no data have so far yielded insights into the metabolic state of Phaeocystis populations that would allow testing this hypothesis. Therefore, endometabolic markers of different growth phases were determined in laboratory batch cultures using comparative metabolomics and quantified in different phytoplankton blooms in the field. Metabolites, produced during exponential, early and late stationary growth of P. pouchetii, were profiled using gas chromatography-mass spectrometry. Then, metabolites were characterized that correlate with the growth phases using multivariate statistical analysis. Free amino acids characterized the exponential growth, whereas the early stationary phase was correlated with sugar alcohols, mono- and disaccharides. In the late stationary phase, free fatty acids, sterols and terpenes increased. These marker metabolites were then traced in Phaeocystis blooms during a cruise in the Barents Sea and North Norwegian fjords. About 50 endometabolites of P. pouchetii were detected in natural phytoplankton communities. Mannitol, scyllo-inositol, 24-methylcholesta-5,22-dien-3β-ol, and several free fatty acids were characteristic for Phaeocystis-dominated blooms but showed variability between them. Distinct metabolic profiles were detected in the nutrient-depleted community in the inner Porsangerfjord (< 0.5 µM NO3 −, < 0.1 µM PO 4 3− ), with high relative amounts of free mono- and disaccharides indicative for a limited culture. This study thereby shows how the variable physiology of phytoplankton can alter the metabolic landscape of entire plankton communities.
format	article
author	Constanze Kuhlisch Julia Althammer Andrey F. Sazhin Hans H. Jakobsen Jens C. Nejstgaard Georg Pohnert
author_facet	Constanze Kuhlisch Julia Althammer Andrey F. Sazhin Hans H. Jakobsen Jens C. Nejstgaard Georg Pohnert
author_sort	Constanze Kuhlisch
title	Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities
title_short	Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities
title_full	Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities
title_fullStr	Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities
title_full_unstemmed	Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities
title_sort	metabolomics-derived marker metabolites to characterize phaeocystis pouchetii physiology in natural plankton communities
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/81f5e761d1294361badc66a4f9b08250
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Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities

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