Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques

Abstract The impact of the post-mortem interval (PMI) on the optical molecular characteristics of the colonic mucosa and the gut-associated lymphoid tissue (GALT) were examined by multi-parametric measurements techniques. Inflammatory cells were identified by immunohistochemical staining. Molecular...

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Autores principales:	Łukasz Zadka, Karolina Chrabaszcz, Igor Buzalewicz, Ewelina Wiercigroch, Natalia Glatzel-Plucińska, Łukasz Szleszkowski, Agnieszka Gomułkiewicz, Aleksandra Piotrowska, Krzysztof Kurnol, Piotr Dzięgiel, Tomasz Jurek, Kamilla Malek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81fc5a51550a445386d5f44a96e7d699
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spelling	oai:doaj.org-article:81fc5a51550a445386d5f44a96e7d6992021-12-02T15:00:59ZMolecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques10.1038/s41598-021-90761-y2045-2322https://doaj.org/article/81fc5a51550a445386d5f44a96e7d6992021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90761-yhttps://doaj.org/toc/2045-2322Abstract The impact of the post-mortem interval (PMI) on the optical molecular characteristics of the colonic mucosa and the gut-associated lymphoid tissue (GALT) were examined by multi-parametric measurements techniques. Inflammatory cells were identified by immunohistochemical staining. Molecular parameters were estimated using the Raman spectroscopy (RS) and Fourier Transform Infrared (FTIR) spectroscopic imaging. The 3D refractive index (3D-RI) distributions of samples were determined using the digital holographic tomography. The distribution of immune cells between post-mortem (PM) and normal controls did show significant differences for CD4 (P = 0.0016) or CD8 (P < 0.0001), whose expression level was decreased in PM cases. No association was found between individual PMI values and inflammatory cell distribution. However, there was a tendency for a negative correlation between CD4+ cells and PMI (r = − 0.542, P = 0.032). The alterations ongoing in post-mortem tissue may suggest that PMI has a suppressive effect on the effector properties of the cell-mediated immunity. Moreover, it was confirmed that spectroscopic and digital holotomographic histology are also a useful technique for characterization of the differences in inflammation of varying intensity and in GALT imaging in a solid tissue. Anatomical location of immune cells and methods of tissue fixation determine the molecular and optical parameters of the examined cases.Łukasz ZadkaKarolina ChrabaszczIgor BuzalewiczEwelina WiercigrochNatalia Glatzel-PlucińskaŁukasz SzleszkowskiAgnieszka GomułkiewiczAleksandra PiotrowskaKrzysztof KurnolPiotr DzięgielTomasz JurekKamilla MalekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Łukasz Zadka Karolina Chrabaszcz Igor Buzalewicz Ewelina Wiercigroch Natalia Glatzel-Plucińska Łukasz Szleszkowski Agnieszka Gomułkiewicz Aleksandra Piotrowska Krzysztof Kurnol Piotr Dzięgiel Tomasz Jurek Kamilla Malek Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
description	Abstract The impact of the post-mortem interval (PMI) on the optical molecular characteristics of the colonic mucosa and the gut-associated lymphoid tissue (GALT) were examined by multi-parametric measurements techniques. Inflammatory cells were identified by immunohistochemical staining. Molecular parameters were estimated using the Raman spectroscopy (RS) and Fourier Transform Infrared (FTIR) spectroscopic imaging. The 3D refractive index (3D-RI) distributions of samples were determined using the digital holographic tomography. The distribution of immune cells between post-mortem (PM) and normal controls did show significant differences for CD4 (P = 0.0016) or CD8 (P < 0.0001), whose expression level was decreased in PM cases. No association was found between individual PMI values and inflammatory cell distribution. However, there was a tendency for a negative correlation between CD4+ cells and PMI (r = − 0.542, P = 0.032). The alterations ongoing in post-mortem tissue may suggest that PMI has a suppressive effect on the effector properties of the cell-mediated immunity. Moreover, it was confirmed that spectroscopic and digital holotomographic histology are also a useful technique for characterization of the differences in inflammation of varying intensity and in GALT imaging in a solid tissue. Anatomical location of immune cells and methods of tissue fixation determine the molecular and optical parameters of the examined cases.
format	article
author	Łukasz Zadka Karolina Chrabaszcz Igor Buzalewicz Ewelina Wiercigroch Natalia Glatzel-Plucińska Łukasz Szleszkowski Agnieszka Gomułkiewicz Aleksandra Piotrowska Krzysztof Kurnol Piotr Dzięgiel Tomasz Jurek Kamilla Malek
author_facet	Łukasz Zadka Karolina Chrabaszcz Igor Buzalewicz Ewelina Wiercigroch Natalia Glatzel-Plucińska Łukasz Szleszkowski Agnieszka Gomułkiewicz Aleksandra Piotrowska Krzysztof Kurnol Piotr Dzięgiel Tomasz Jurek Kamilla Malek
author_sort	Łukasz Zadka
title	Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
title_short	Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
title_full	Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
title_fullStr	Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
title_full_unstemmed	Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
title_sort	molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/81fc5a51550a445386d5f44a96e7d699
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Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques

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