Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance

Abstract Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eun-Ah Christine Song, Sangwon Min, Akinsola Oyelakin, Kirsten Smalley, Jonathan E. Bard, Lan Liao, Jianming Xu, Rose-Anne Romano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Myoepithelial Cells (MECs) Myoepithelial Cell Population Adult Gland Genetic Lineage Tracing SG Morphogenesis Medicine R Science Q
Acceso en línea:	https://doaj.org/article/81fd0be0967c4116aa8e1c15063e61e5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:81fd0be0967c4116aa8e1c15063e61e5
record_format	dspace
spelling	oai:doaj.org-article:81fd0be0967c4116aa8e1c15063e61e52021-12-02T15:08:00ZGenetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance10.1038/s41598-018-32343-z2045-2322https://doaj.org/article/81fd0be0967c4116aa8e1c15063e61e52018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32343-zhttps://doaj.org/toc/2045-2322Abstract Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing and in vivo genetic lineage tracing technologies to generate a detailed map of the cell fate trajectories and branch points of the basal and myoepithelial cell populations of the mouse SMG during embryonic development and in adults. Our studies show that the transcription factor p63 and alpha-smooth muscle actin (SMA) serve as faithful markers of the basal and myoepithelial cell lineages, respectively and that both cell types are endowed with progenitor cell properties. However, p63+ basal and SMA+ myoepithelial cells exhibit distinct cell fates by virtue of maintaining different cellular lineages during morphogenesis and in adults. Collectively, our results reveal the dynamic and complex nature of the diverse SMG cell populations and highlight the distinct differentiation potential of the p63 and SMA expressing subtypes in the stem and progenitor cell hierarchy. Long term these findings have profound implications towards a better understanding of the molecular mechanisms that dictate lineage commitment and differentiation programs during development and adult gland maintenance.Eun-Ah Christine SongSangwon MinAkinsola OyelakinKirsten SmalleyJonathan E. BardLan LiaoJianming XuRose-Anne RomanoNature PortfolioarticleMyoepithelial Cells (MECs)Myoepithelial Cell PopulationAdult GlandGenetic Lineage TracingSG MorphogenesisMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Myoepithelial Cells (MECs) Myoepithelial Cell Population Adult Gland Genetic Lineage Tracing SG Morphogenesis Medicine R Science Q
spellingShingle	Myoepithelial Cells (MECs) Myoepithelial Cell Population Adult Gland Genetic Lineage Tracing SG Morphogenesis Medicine R Science Q Eun-Ah Christine Song Sangwon Min Akinsola Oyelakin Kirsten Smalley Jonathan E. Bard Lan Liao Jianming Xu Rose-Anne Romano Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
description	Abstract Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing and in vivo genetic lineage tracing technologies to generate a detailed map of the cell fate trajectories and branch points of the basal and myoepithelial cell populations of the mouse SMG during embryonic development and in adults. Our studies show that the transcription factor p63 and alpha-smooth muscle actin (SMA) serve as faithful markers of the basal and myoepithelial cell lineages, respectively and that both cell types are endowed with progenitor cell properties. However, p63+ basal and SMA+ myoepithelial cells exhibit distinct cell fates by virtue of maintaining different cellular lineages during morphogenesis and in adults. Collectively, our results reveal the dynamic and complex nature of the diverse SMG cell populations and highlight the distinct differentiation potential of the p63 and SMA expressing subtypes in the stem and progenitor cell hierarchy. Long term these findings have profound implications towards a better understanding of the molecular mechanisms that dictate lineage commitment and differentiation programs during development and adult gland maintenance.
format	article
author	Eun-Ah Christine Song Sangwon Min Akinsola Oyelakin Kirsten Smalley Jonathan E. Bard Lan Liao Jianming Xu Rose-Anne Romano
author_facet	Eun-Ah Christine Song Sangwon Min Akinsola Oyelakin Kirsten Smalley Jonathan E. Bard Lan Liao Jianming Xu Rose-Anne Romano
author_sort	Eun-Ah Christine Song
title	Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_short	Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_full	Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_fullStr	Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_full_unstemmed	Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_sort	genetic and scrna-seq analysis reveals distinct cell populations that contribute to salivary gland development and maintenance
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/81fd0be0967c4116aa8e1c15063e61e5
work_keys_str_mv	AT eunahchristinesong geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT sangwonmin geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT akinsolaoyelakin geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT kirstensmalley geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT jonathanebard geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT lanliao geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT jianmingxu geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance AT roseanneromano geneticandscrnaseqanalysisrevealsdistinctcellpopulationsthatcontributetosalivaryglanddevelopmentandmaintenance
_version_	1718388326473400320

Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance

Ejemplares similares