A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.

Saxitoxin and its analogs cause the paralytic shellfish-poisoning syndrome, adversely affecting human health and coastal shellfish industries worldwide. Here we report the isolation, sequencing, annotation, and predicted pathway of the saxitoxin biosynthetic gene cluster in the cyanobacterium Lyngby...

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Autores principales:	Troco K Mihali, Wayne W Carmichael, Brett A Neilan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8210d02a9d9c4b84ac7d1aaa0b17c06e
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spelling	oai:doaj.org-article:8210d02a9d9c4b84ac7d1aaa0b17c06e2021-11-18T06:58:56ZA putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.1932-620310.1371/journal.pone.0014657https://doaj.org/article/8210d02a9d9c4b84ac7d1aaa0b17c06e2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21347365/?tool=EBIhttps://doaj.org/toc/1932-6203Saxitoxin and its analogs cause the paralytic shellfish-poisoning syndrome, adversely affecting human health and coastal shellfish industries worldwide. Here we report the isolation, sequencing, annotation, and predicted pathway of the saxitoxin biosynthetic gene cluster in the cyanobacterium Lyngbya wollei. The gene cluster spans 36 kb and encodes enzymes for the biosynthesis and export of the toxins. The Lyngbya wollei saxitoxin gene cluster differs from previously identified saxitoxin clusters as it contains genes that are unique to this cluster, whereby the carbamoyltransferase is truncated and replaced by an acyltransferase, explaining the unique toxin profile presented by Lyngbya wollei. These findings will enable the creation of toxin probes, for water monitoring purposes, as well as proof-of-concept for the combinatorial biosynthesis of these natural occurring alkaloids for the production of novel, biologically active compounds.Troco K MihaliWayne W CarmichaelBrett A NeilanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e14657 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Troco K Mihali Wayne W Carmichael Brett A Neilan A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
description	Saxitoxin and its analogs cause the paralytic shellfish-poisoning syndrome, adversely affecting human health and coastal shellfish industries worldwide. Here we report the isolation, sequencing, annotation, and predicted pathway of the saxitoxin biosynthetic gene cluster in the cyanobacterium Lyngbya wollei. The gene cluster spans 36 kb and encodes enzymes for the biosynthesis and export of the toxins. The Lyngbya wollei saxitoxin gene cluster differs from previously identified saxitoxin clusters as it contains genes that are unique to this cluster, whereby the carbamoyltransferase is truncated and replaced by an acyltransferase, explaining the unique toxin profile presented by Lyngbya wollei. These findings will enable the creation of toxin probes, for water monitoring purposes, as well as proof-of-concept for the combinatorial biosynthesis of these natural occurring alkaloids for the production of novel, biologically active compounds.
format	article
author	Troco K Mihali Wayne W Carmichael Brett A Neilan
author_facet	Troco K Mihali Wayne W Carmichael Brett A Neilan
author_sort	Troco K Mihali
title	A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
title_short	A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
title_full	A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
title_fullStr	A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
title_full_unstemmed	A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
title_sort	putative gene cluster from a lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8210d02a9d9c4b84ac7d1aaa0b17c06e
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A putative gene cluster from a Lyngbya wollei bloom that encodes paralytic shellfish toxin biosynthesis.

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