Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community comp...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/8217c41b26f14d389b8a856bfd67054e |
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| Sumario: | The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community composition, and diversity under different N application rates in a water-limited area. We investigated the bacterial abundance, diversity, community composition, and structure under five different N gradients (0, 90, 150, 210, and 270 kg ha<sup>−1</sup>) using real-time quantitative PCR and high-throughput sequencing, and then explored bacterial functional groups with FAPROTAX. N application significantly affected bacterial abundance and community composition. Bacterial diversity was enhanced at low N application rates and reduced at higher N application rates. Principal coordinate analysis showed that bacterial community structure was separated into two groups between low N application rates and high N application rates; these differences in bacterial community structure may be driven by available nitrogen (AN). The results of FAPROTAX revealed that N application promoted the functions of Aerobic_nitrite_oxidation, Nitrate_reduction, and Aerobic_ammonia_oxidation, but inhibited the Nitrogen_fixation function of the bacterial community. The high N network caused the reduction of network structure stability. Our results revealed that N fertilizer driven bacterial community structure and soil nutrients were the main influential factors in the variation of bacterial community structure. We suggest that the optimal N application rate in this study may be approximately 150 kg ha<sup>−1</sup>, based on the variations of soil properties and bacterial community structure in semi-arid areas. |
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