Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China

The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community comp...

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Autores principales:	Meng Wang, Ling Wang, Qian Li, Hang Liu, Yuan Lin, Lichun Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacterial community Illumina MiSeq sequencing nitrogen fertilization soil properties Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/8217c41b26f14d389b8a856bfd67054e
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spelling	oai:doaj.org-article:8217c41b26f14d389b8a856bfd67054e2021-11-11T19:38:33ZNitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China10.3390/su1321119672071-1050https://doaj.org/article/8217c41b26f14d389b8a856bfd67054e2021-10-01T00:00:00Zhttps://www.mdpi.com/2071-1050/13/21/11967https://doaj.org/toc/2071-1050The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community composition, and diversity under different N application rates in a water-limited area. We investigated the bacterial abundance, diversity, community composition, and structure under five different N gradients (0, 90, 150, 210, and 270 kg ha<sup>−1</sup>) using real-time quantitative PCR and high-throughput sequencing, and then explored bacterial functional groups with FAPROTAX. N application significantly affected bacterial abundance and community composition. Bacterial diversity was enhanced at low N application rates and reduced at higher N application rates. Principal coordinate analysis showed that bacterial community structure was separated into two groups between low N application rates and high N application rates; these differences in bacterial community structure may be driven by available nitrogen (AN). The results of FAPROTAX revealed that N application promoted the functions of Aerobic_nitrite_oxidation, Nitrate_reduction, and Aerobic_ammonia_oxidation, but inhibited the Nitrogen_fixation function of the bacterial community. The high N network caused the reduction of network structure stability. Our results revealed that N fertilizer driven bacterial community structure and soil nutrients were the main influential factors in the variation of bacterial community structure. We suggest that the optimal N application rate in this study may be approximately 150 kg ha<sup>−1</sup>, based on the variations of soil properties and bacterial community structure in semi-arid areas.Meng WangLing WangQian LiHang LiuYuan LinLichun WangMDPI AGarticlebacterial communityIllumina MiSeq sequencingnitrogen fertilizationsoil propertiesEnvironmental effects of industries and plantsTD194-195Renewable energy sourcesTJ807-830Environmental sciencesGE1-350ENSustainability, Vol 13, Iss 11967, p 11967 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial community Illumina MiSeq sequencing nitrogen fertilization soil properties Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350
spellingShingle	bacterial community Illumina MiSeq sequencing nitrogen fertilization soil properties Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 Meng Wang Ling Wang Qian Li Hang Liu Yuan Lin Lichun Wang Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
description	The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community composition, and diversity under different N application rates in a water-limited area. We investigated the bacterial abundance, diversity, community composition, and structure under five different N gradients (0, 90, 150, 210, and 270 kg ha<sup>−1</sup>) using real-time quantitative PCR and high-throughput sequencing, and then explored bacterial functional groups with FAPROTAX. N application significantly affected bacterial abundance and community composition. Bacterial diversity was enhanced at low N application rates and reduced at higher N application rates. Principal coordinate analysis showed that bacterial community structure was separated into two groups between low N application rates and high N application rates; these differences in bacterial community structure may be driven by available nitrogen (AN). The results of FAPROTAX revealed that N application promoted the functions of Aerobic_nitrite_oxidation, Nitrate_reduction, and Aerobic_ammonia_oxidation, but inhibited the Nitrogen_fixation function of the bacterial community. The high N network caused the reduction of network structure stability. Our results revealed that N fertilizer driven bacterial community structure and soil nutrients were the main influential factors in the variation of bacterial community structure. We suggest that the optimal N application rate in this study may be approximately 150 kg ha<sup>−1</sup>, based on the variations of soil properties and bacterial community structure in semi-arid areas.
format	article
author	Meng Wang Ling Wang Qian Li Hang Liu Yuan Lin Lichun Wang
author_facet	Meng Wang Ling Wang Qian Li Hang Liu Yuan Lin Lichun Wang
author_sort	Meng Wang
title	Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
title_short	Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
title_full	Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
title_fullStr	Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
title_full_unstemmed	Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China
title_sort	nitrogen fertilizer driven bacterial community structure in a semi-arid region of northeast china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8217c41b26f14d389b8a856bfd67054e
work_keys_str_mv	AT mengwang nitrogenfertilizerdrivenbacterialcommunitystructureinasemiaridregionofnortheastchina AT lingwang nitrogenfertilizerdrivenbacterialcommunitystructureinasemiaridregionofnortheastchina AT qianli nitrogenfertilizerdrivenbacterialcommunitystructureinasemiaridregionofnortheastchina AT hangliu nitrogenfertilizerdrivenbacterialcommunitystructureinasemiaridregionofnortheastchina AT yuanlin nitrogenfertilizerdrivenbacterialcommunitystructureinasemiaridregionofnortheastchina AT lichunwang nitrogenfertilizerdrivenbacterialcommunitystructureinasemiaridregionofnortheastchina
_version_	1718431477106999296

Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China

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