High human bocavirus viral load is associated with disease severity in children under five years of age.

Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patien...

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Autores principales:	Baihui Zhao, Xuelian Yu, Chuanxian Wang, Zheng Teng, Chun Wang, Jiaren Shen, Ye Gao, Zhaokui Zhu, Jiayu Wang, Zhengan Yuan, Fan Wu, Xi Zhang, Reena Ghildyal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/822180592a1241cbb5a0ebb1432854de
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spelling	oai:doaj.org-article:822180592a1241cbb5a0ebb1432854de2021-11-18T07:47:31ZHigh human bocavirus viral load is associated with disease severity in children under five years of age.1932-620310.1371/journal.pone.0062318https://doaj.org/article/822180592a1241cbb5a0ebb1432854de2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638038/?tool=EBIhttps://doaj.org/toc/1932-6203Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1-4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0% of inpatients, with annual rates of 5.1%, 8.0% and 4.8% in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>10(6) copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load.Baihui ZhaoXuelian YuChuanxian WangZheng TengChun WangJiaren ShenYe GaoZhaokui ZhuJiayu WangZhengan YuanFan WuXi ZhangReena GhildyalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62318 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Baihui Zhao Xuelian Yu Chuanxian Wang Zheng Teng Chun Wang Jiaren Shen Ye Gao Zhaokui Zhu Jiayu Wang Zhengan Yuan Fan Wu Xi Zhang Reena Ghildyal High human bocavirus viral load is associated with disease severity in children under five years of age.
description	Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1-4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0% of inpatients, with annual rates of 5.1%, 8.0% and 4.8% in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>10(6) copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load.
format	article
author	Baihui Zhao Xuelian Yu Chuanxian Wang Zheng Teng Chun Wang Jiaren Shen Ye Gao Zhaokui Zhu Jiayu Wang Zhengan Yuan Fan Wu Xi Zhang Reena Ghildyal
author_facet	Baihui Zhao Xuelian Yu Chuanxian Wang Zheng Teng Chun Wang Jiaren Shen Ye Gao Zhaokui Zhu Jiayu Wang Zhengan Yuan Fan Wu Xi Zhang Reena Ghildyal
author_sort	Baihui Zhao
title	High human bocavirus viral load is associated with disease severity in children under five years of age.
title_short	High human bocavirus viral load is associated with disease severity in children under five years of age.
title_full	High human bocavirus viral load is associated with disease severity in children under five years of age.
title_fullStr	High human bocavirus viral load is associated with disease severity in children under five years of age.
title_full_unstemmed	High human bocavirus viral load is associated with disease severity in children under five years of age.
title_sort	high human bocavirus viral load is associated with disease severity in children under five years of age.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/822180592a1241cbb5a0ebb1432854de
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High human bocavirus viral load is associated with disease severity in children under five years of age.

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