HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes

Abstract Skeletal muscle exhibits a high regenerative capacity, mainly due to the ability of satellite cells to replicate and differentiate in response to appropriate stimuli. Epigenetic control is effective at different stages of this process. It has been shown that the chromatin-remodeling factor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicoletta Marroncelli, Marzia Bianchi, Marco Bertin, Silvia Consalvi, Valentina Saccone, Marco De Bardi, Pier Lorenzo Puri, Daniela Palacios, Sergio Adamo, Viviana Moresi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8222ae1687854e2c9eb12bbfa1421b9b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8222ae1687854e2c9eb12bbfa1421b9b
record_format	dspace
spelling	oai:doaj.org-article:8222ae1687854e2c9eb12bbfa1421b9b2021-12-02T12:32:48ZHDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes10.1038/s41598-018-21835-72045-2322https://doaj.org/article/8222ae1687854e2c9eb12bbfa1421b9b2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21835-7https://doaj.org/toc/2045-2322Abstract Skeletal muscle exhibits a high regenerative capacity, mainly due to the ability of satellite cells to replicate and differentiate in response to appropriate stimuli. Epigenetic control is effective at different stages of this process. It has been shown that the chromatin-remodeling factor HDAC4 is able to regulate satellite cell proliferation and commitment. However, its molecular targets are still uncovered. To explain the signaling pathways regulated by HDAC4 in satellite cells, we generated tamoxifen-inducible mice with conditional inactivation of HDAC4 in Pax7+ cells (HDAC4 KO mice). We found that the proliferation and differentiation of HDAC4 KO satellite cells were compromised, although similar amounts of satellite cells were found in mice. Moreover, we found that the inhibition of HDAC4 in satellite cells was sufficient to block the differentiation process. By RNA-sequencing analysis we identified P21 and Sharp1 as HDAC4 target genes. Reducing the expression of these target genes in HDAC4 KO satellite cells, we also defined the molecular pathways regulated by HDAC4 in the epigenetic control of satellite cell expansion and fusion.Nicoletta MarroncelliMarzia BianchiMarco BertinSilvia ConsalviValentina SacconeMarco De BardiPier Lorenzo PuriDaniela PalaciosSergio AdamoViviana MoresiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicoletta Marroncelli Marzia Bianchi Marco Bertin Silvia Consalvi Valentina Saccone Marco De Bardi Pier Lorenzo Puri Daniela Palacios Sergio Adamo Viviana Moresi HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes
description	Abstract Skeletal muscle exhibits a high regenerative capacity, mainly due to the ability of satellite cells to replicate and differentiate in response to appropriate stimuli. Epigenetic control is effective at different stages of this process. It has been shown that the chromatin-remodeling factor HDAC4 is able to regulate satellite cell proliferation and commitment. However, its molecular targets are still uncovered. To explain the signaling pathways regulated by HDAC4 in satellite cells, we generated tamoxifen-inducible mice with conditional inactivation of HDAC4 in Pax7+ cells (HDAC4 KO mice). We found that the proliferation and differentiation of HDAC4 KO satellite cells were compromised, although similar amounts of satellite cells were found in mice. Moreover, we found that the inhibition of HDAC4 in satellite cells was sufficient to block the differentiation process. By RNA-sequencing analysis we identified P21 and Sharp1 as HDAC4 target genes. Reducing the expression of these target genes in HDAC4 KO satellite cells, we also defined the molecular pathways regulated by HDAC4 in the epigenetic control of satellite cell expansion and fusion.
format	article
author	Nicoletta Marroncelli Marzia Bianchi Marco Bertin Silvia Consalvi Valentina Saccone Marco De Bardi Pier Lorenzo Puri Daniela Palacios Sergio Adamo Viviana Moresi
author_facet	Nicoletta Marroncelli Marzia Bianchi Marco Bertin Silvia Consalvi Valentina Saccone Marco De Bardi Pier Lorenzo Puri Daniela Palacios Sergio Adamo Viviana Moresi
author_sort	Nicoletta Marroncelli
title	HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes
title_short	HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes
title_full	HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes
title_fullStr	HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes
title_full_unstemmed	HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes
title_sort	hdac4 regulates satellite cell proliferation and differentiation by targeting p21 and sharp1 genes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8222ae1687854e2c9eb12bbfa1421b9b
work_keys_str_mv	AT nicolettamarroncelli hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT marziabianchi hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT marcobertin hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT silviaconsalvi hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT valentinasaccone hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT marcodebardi hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT pierlorenzopuri hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT danielapalacios hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT sergioadamo hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes AT vivianamoresi hdac4regulatessatellitecellproliferationanddifferentiationbytargetingp21andsharp1genes
_version_	1718393989384634368

HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes

Ejemplares similares