Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses

Both H5N1 and H7N9 subtype avian influenza viruses cause enormous economic losses and pose considerable threats to public health. Bivalent vaccines against both two subtypes are more effective in control of H5N1 and H7N9 viruses in poultry and novel egg-independent vaccines are needed. Herein, H5 an...

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Autores principales:	Jiao Hu, Peipei Peng, Jun Li, Qi Zhang, Rumeng Li, Xiaoquan Wang, Min Gu, Zenglei Hu, Shunlin Hu, Xiaowen Liu, Xinan Jiao, Daxin Peng, Xiufan Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	avian influenza virus H5N1 subtype H7N9 subtype virus-like particle baculovirus expression system bivalent vaccine Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/8232c09a8f214d258b61fe084147645b
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spelling	oai:doaj.org-article:8232c09a8f214d258b61fe084147645b2021-11-11T08:07:25ZSingle Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses2297-176910.3389/fvets.2021.774630https://doaj.org/article/8232c09a8f214d258b61fe084147645b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.774630/fullhttps://doaj.org/toc/2297-1769Both H5N1 and H7N9 subtype avian influenza viruses cause enormous economic losses and pose considerable threats to public health. Bivalent vaccines against both two subtypes are more effective in control of H5N1 and H7N9 viruses in poultry and novel egg-independent vaccines are needed. Herein, H5 and H7 virus like particle (VLP) were generated in a baculovirus expression system and a bivalent H5+H7 VLP vaccine candidate was prepared by combining these two antigens. Single immunization of the bivalent VLP or commercial inactivated vaccines elicited effective antibody immune responses, including hemagglutination inhibition, virus neutralizing and HA-specific IgG antibodies. All vaccinated birds survived lethal challenge with highly pathogenic H5N1 and H7N9 viruses. Furthermore, the bivalent VLP significantly reduced viral shedding and virus replication in chickens, which was comparable to that observed for the commercial inactivated vaccine. However, the bivalent VLP was better than the commercial vaccine in terms of alleviating pulmonary lesions caused by H7N9 virus infection in chickens. Therefore, our study suggests that the bivalent H5+H7 VLP vaccine candidate can serve as a critical alternative for the traditional egg-based inactivated vaccines against H5N1 and H7N9 avian influenza virus infection in poultry.Jiao HuJiao HuJiao HuPeipei PengPeipei PengPeipei PengJun LiJun LiJun LiQi ZhangQi ZhangQi ZhangRumeng LiRumeng LiRumeng LiXiaoquan WangXiaoquan WangXiaoquan WangMin GuMin GuMin GuZenglei HuZenglei HuZenglei HuShunlin HuShunlin HuShunlin HuXiaowen LiuXiaowen LiuXiaowen LiuXinan JiaoXinan JiaoDaxin PengDaxin PengDaxin PengXiufan LiuXiufan LiuXiufan LiuFrontiers Media S.A.articleavian influenza virusH5N1 subtypeH7N9 subtypevirus-like particlebaculovirus expression systembivalent vaccineVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	avian influenza virus H5N1 subtype H7N9 subtype virus-like particle baculovirus expression system bivalent vaccine Veterinary medicine SF600-1100
spellingShingle	avian influenza virus H5N1 subtype H7N9 subtype virus-like particle baculovirus expression system bivalent vaccine Veterinary medicine SF600-1100 Jiao Hu Jiao Hu Jiao Hu Peipei Peng Peipei Peng Peipei Peng Jun Li Jun Li Jun Li Qi Zhang Qi Zhang Qi Zhang Rumeng Li Rumeng Li Rumeng Li Xiaoquan Wang Xiaoquan Wang Xiaoquan Wang Min Gu Min Gu Min Gu Zenglei Hu Zenglei Hu Zenglei Hu Shunlin Hu Shunlin Hu Shunlin Hu Xiaowen Liu Xiaowen Liu Xiaowen Liu Xinan Jiao Xinan Jiao Daxin Peng Daxin Peng Daxin Peng Xiufan Liu Xiufan Liu Xiufan Liu Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses
description	Both H5N1 and H7N9 subtype avian influenza viruses cause enormous economic losses and pose considerable threats to public health. Bivalent vaccines against both two subtypes are more effective in control of H5N1 and H7N9 viruses in poultry and novel egg-independent vaccines are needed. Herein, H5 and H7 virus like particle (VLP) were generated in a baculovirus expression system and a bivalent H5+H7 VLP vaccine candidate was prepared by combining these two antigens. Single immunization of the bivalent VLP or commercial inactivated vaccines elicited effective antibody immune responses, including hemagglutination inhibition, virus neutralizing and HA-specific IgG antibodies. All vaccinated birds survived lethal challenge with highly pathogenic H5N1 and H7N9 viruses. Furthermore, the bivalent VLP significantly reduced viral shedding and virus replication in chickens, which was comparable to that observed for the commercial inactivated vaccine. However, the bivalent VLP was better than the commercial vaccine in terms of alleviating pulmonary lesions caused by H7N9 virus infection in chickens. Therefore, our study suggests that the bivalent H5+H7 VLP vaccine candidate can serve as a critical alternative for the traditional egg-based inactivated vaccines against H5N1 and H7N9 avian influenza virus infection in poultry.
format	article
author	Jiao Hu Jiao Hu Jiao Hu Peipei Peng Peipei Peng Peipei Peng Jun Li Jun Li Jun Li Qi Zhang Qi Zhang Qi Zhang Rumeng Li Rumeng Li Rumeng Li Xiaoquan Wang Xiaoquan Wang Xiaoquan Wang Min Gu Min Gu Min Gu Zenglei Hu Zenglei Hu Zenglei Hu Shunlin Hu Shunlin Hu Shunlin Hu Xiaowen Liu Xiaowen Liu Xiaowen Liu Xinan Jiao Xinan Jiao Daxin Peng Daxin Peng Daxin Peng Xiufan Liu Xiufan Liu Xiufan Liu
author_facet	Jiao Hu Jiao Hu Jiao Hu Peipei Peng Peipei Peng Peipei Peng Jun Li Jun Li Jun Li Qi Zhang Qi Zhang Qi Zhang Rumeng Li Rumeng Li Rumeng Li Xiaoquan Wang Xiaoquan Wang Xiaoquan Wang Min Gu Min Gu Min Gu Zenglei Hu Zenglei Hu Zenglei Hu Shunlin Hu Shunlin Hu Shunlin Hu Xiaowen Liu Xiaowen Liu Xiaowen Liu Xinan Jiao Xinan Jiao Daxin Peng Daxin Peng Daxin Peng Xiufan Liu Xiufan Liu Xiufan Liu
author_sort	Jiao Hu
title	Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses
title_short	Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses
title_full	Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses
title_fullStr	Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses
title_full_unstemmed	Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses
title_sort	single dose of bivalent h5 and h7 influenza virus-like particle protects chickens against highly pathogenic h5n1 and h7n9 avian influenza viruses
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8232c09a8f214d258b61fe084147645b
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Single Dose of Bivalent H5 and H7 Influenza Virus-Like Particle Protects Chickens Against Highly Pathogenic H5N1 and H7N9 Avian Influenza Viruses

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