Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an important emerging zoonotic pathogen that causes severe skin infections. To combat infections from drug-resistant bacteria, the transplantation of commensal antimicrobial bacteria as a therapeutic has shown clinical promise. We scree...

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Autores principales:	Alan M O'Neill, Kate A Worthing, Nikhil Kulkarni, Fengwu Li, Teruaki Nakatsuji, Dominic McGrosso, Robert H Mills, Gayathri Kalla, Joyce Y Cheng, Jacqueline M Norris, Kit Pogliano, Joe Pogliano, David J Gonzalez, Richard L Gallo
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	staphylococcus felis Staphylococcus aureus staphylococcus pseudintermedius skin infection antimicrobial Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8239653fab1c46dc8610832c2aa22e68
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spelling	oai:doaj.org-article:8239653fab1c46dc8610832c2aa22e682021-11-15T05:42:20ZAntimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius10.7554/eLife.667932050-084Xe66793https://doaj.org/article/8239653fab1c46dc8610832c2aa22e682021-10-01T00:00:00Zhttps://elifesciences.org/articles/66793https://doaj.org/toc/2050-084XMethicillin-resistant Staphylococcus pseudintermedius (MRSP) is an important emerging zoonotic pathogen that causes severe skin infections. To combat infections from drug-resistant bacteria, the transplantation of commensal antimicrobial bacteria as a therapeutic has shown clinical promise. We screened a collection of diverse staphylococcus species from domestic dogs and cats for antimicrobial activity against MRSP. A unique strain (S. felis C4) was isolated from feline skin that inhibited MRSP and multiple gram-positive pathogens. Whole genome sequencing and mass spectrometry revealed several secreted antimicrobials including a thiopeptide bacteriocin micrococcin P1 and phenol-soluble modulin beta (PSMβ) peptides that exhibited antimicrobial and anti-inflammatory activity. Fluorescence and electron microscopy revealed that S. felis antimicrobials inhibited translation and disrupted bacterial but not eukaryotic cell membranes. Competition experiments in mice showed that S. felis significantly reduced MRSP skin colonization and an antimicrobial extract from S. felis significantly reduced necrotic skin injury from MRSP infection. These findings indicate a feline commensal bacterium that could be utilized in bacteriotherapy against difficult-to-treat animal and human skin infections.Alan M O'NeillKate A WorthingNikhil KulkarniFengwu LiTeruaki NakatsujiDominic McGrossoRobert H MillsGayathri KallaJoyce Y ChengJacqueline M NorrisKit PoglianoJoe PoglianoDavid J GonzalezRichard L GalloeLife Sciences Publications Ltdarticlestaphylococcus felisStaphylococcus aureusstaphylococcus pseudintermediusskininfectionantimicrobialMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	staphylococcus felis Staphylococcus aureus staphylococcus pseudintermedius skin infection antimicrobial Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	staphylococcus felis Staphylococcus aureus staphylococcus pseudintermedius skin infection antimicrobial Medicine R Science Q Biology (General) QH301-705.5 Alan M O'Neill Kate A Worthing Nikhil Kulkarni Fengwu Li Teruaki Nakatsuji Dominic McGrosso Robert H Mills Gayathri Kalla Joyce Y Cheng Jacqueline M Norris Kit Pogliano Joe Pogliano David J Gonzalez Richard L Gallo Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius
description	Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an important emerging zoonotic pathogen that causes severe skin infections. To combat infections from drug-resistant bacteria, the transplantation of commensal antimicrobial bacteria as a therapeutic has shown clinical promise. We screened a collection of diverse staphylococcus species from domestic dogs and cats for antimicrobial activity against MRSP. A unique strain (S. felis C4) was isolated from feline skin that inhibited MRSP and multiple gram-positive pathogens. Whole genome sequencing and mass spectrometry revealed several secreted antimicrobials including a thiopeptide bacteriocin micrococcin P1 and phenol-soluble modulin beta (PSMβ) peptides that exhibited antimicrobial and anti-inflammatory activity. Fluorescence and electron microscopy revealed that S. felis antimicrobials inhibited translation and disrupted bacterial but not eukaryotic cell membranes. Competition experiments in mice showed that S. felis significantly reduced MRSP skin colonization and an antimicrobial extract from S. felis significantly reduced necrotic skin injury from MRSP infection. These findings indicate a feline commensal bacterium that could be utilized in bacteriotherapy against difficult-to-treat animal and human skin infections.
format	article
author	Alan M O'Neill Kate A Worthing Nikhil Kulkarni Fengwu Li Teruaki Nakatsuji Dominic McGrosso Robert H Mills Gayathri Kalla Joyce Y Cheng Jacqueline M Norris Kit Pogliano Joe Pogliano David J Gonzalez Richard L Gallo
author_facet	Alan M O'Neill Kate A Worthing Nikhil Kulkarni Fengwu Li Teruaki Nakatsuji Dominic McGrosso Robert H Mills Gayathri Kalla Joyce Y Cheng Jacqueline M Norris Kit Pogliano Joe Pogliano David J Gonzalez Richard L Gallo
author_sort	Alan M O'Neill
title	Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius
title_short	Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius
title_full	Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius
title_fullStr	Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius
title_full_unstemmed	Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius
title_sort	antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant s. pseudintermedius
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/8239653fab1c46dc8610832c2aa22e68
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Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistant S. pseudintermedius

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