Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.

<h4>Background</h4>Dyslipoproteinemia, obesity and insulin resistance are integrative constituents of the metabolic syndrome and are major risk factors for hypertension. The objective of this study was to determine whether hypertension specifically affects the plasma lipidome independent...

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Autores principales:	Juergen Graessler, Dominik Schwudke, Peter E H Schwarz, Ronny Herzog, Andrej Shevchenko, Stefan R Bornstein
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8244679a38a84012bdc2ddfa8fa8758e
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spelling	oai:doaj.org-article:8244679a38a84012bdc2ddfa8fa8758e2021-11-25T06:21:37ZTop-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.1932-620310.1371/journal.pone.0006261https://doaj.org/article/8244679a38a84012bdc2ddfa8fa8758e2009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19603071/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dyslipoproteinemia, obesity and insulin resistance are integrative constituents of the metabolic syndrome and are major risk factors for hypertension. The objective of this study was to determine whether hypertension specifically affects the plasma lipidome independently and differently from the effects induced by obesity and insulin resistance.<h4>Methodology/principal findings</h4>We screened the plasma lipidome of 19 men with hypertension and 51 normotensive male controls by top-down shotgun profiling on a LTQ Orbitrap hybrid mass spectrometer. The analysis encompassed 95 lipid species of 10 major lipid classes. Obesity resulted in generally higher lipid load in blood plasma, while the content of tri- and diacylglycerols increased dramatically. Insulin resistance, defined by HOMA-IR >3.5 and controlled for BMI, had little effect on the plasma lipidome. Importantly, we observed that in blood plasma of hypertensive individuals the overall content of ether lipids decreased. Ether phosphatidylcholines and ether phosphatidylethanolamines, that comprise arachidonic (20:4) and docosapentaenoic (22:5) fatty acid moieties, were specifically diminished. The content of free cholesterol also decreased, although conventional clinical lipid homeostasis indices remained unaffected.<h4>Conclusions/significance</h4>Top-down shotgun lipidomics demonstrated that hypertension is accompanied by specific reduction of the content of ether lipids and free cholesterol that occurred independently of lipidomic alterations induced by obesity and insulin resistance. These results may form the basis for novel preventive and dietary strategies alleviating the severity of hypertension.Juergen GraesslerDominik SchwudkePeter E H SchwarzRonny HerzogAndrej ShevchenkoStefan R BornsteinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6261 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juergen Graessler Dominik Schwudke Peter E H Schwarz Ronny Herzog Andrej Shevchenko Stefan R Bornstein Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
description	<h4>Background</h4>Dyslipoproteinemia, obesity and insulin resistance are integrative constituents of the metabolic syndrome and are major risk factors for hypertension. The objective of this study was to determine whether hypertension specifically affects the plasma lipidome independently and differently from the effects induced by obesity and insulin resistance.<h4>Methodology/principal findings</h4>We screened the plasma lipidome of 19 men with hypertension and 51 normotensive male controls by top-down shotgun profiling on a LTQ Orbitrap hybrid mass spectrometer. The analysis encompassed 95 lipid species of 10 major lipid classes. Obesity resulted in generally higher lipid load in blood plasma, while the content of tri- and diacylglycerols increased dramatically. Insulin resistance, defined by HOMA-IR >3.5 and controlled for BMI, had little effect on the plasma lipidome. Importantly, we observed that in blood plasma of hypertensive individuals the overall content of ether lipids decreased. Ether phosphatidylcholines and ether phosphatidylethanolamines, that comprise arachidonic (20:4) and docosapentaenoic (22:5) fatty acid moieties, were specifically diminished. The content of free cholesterol also decreased, although conventional clinical lipid homeostasis indices remained unaffected.<h4>Conclusions/significance</h4>Top-down shotgun lipidomics demonstrated that hypertension is accompanied by specific reduction of the content of ether lipids and free cholesterol that occurred independently of lipidomic alterations induced by obesity and insulin resistance. These results may form the basis for novel preventive and dietary strategies alleviating the severity of hypertension.
format	article
author	Juergen Graessler Dominik Schwudke Peter E H Schwarz Ronny Herzog Andrej Shevchenko Stefan R Bornstein
author_facet	Juergen Graessler Dominik Schwudke Peter E H Schwarz Ronny Herzog Andrej Shevchenko Stefan R Bornstein
author_sort	Juergen Graessler
title	Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
title_short	Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
title_full	Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
title_fullStr	Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
title_full_unstemmed	Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
title_sort	top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/8244679a38a84012bdc2ddfa8fa8758e
work_keys_str_mv	AT juergengraessler topdownlipidomicsrevealsetherlipiddeficiencyinbloodplasmaofhypertensivepatients AT dominikschwudke topdownlipidomicsrevealsetherlipiddeficiencyinbloodplasmaofhypertensivepatients AT peterehschwarz topdownlipidomicsrevealsetherlipiddeficiencyinbloodplasmaofhypertensivepatients AT ronnyherzog topdownlipidomicsrevealsetherlipiddeficiencyinbloodplasmaofhypertensivepatients AT andrejshevchenko topdownlipidomicsrevealsetherlipiddeficiencyinbloodplasmaofhypertensivepatients AT stefanrbornstein topdownlipidomicsrevealsetherlipiddeficiencyinbloodplasmaofhypertensivepatients
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Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients.

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