Facile isolation and analysis of sporopollenin exine from bee pollen

Abstract We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules...

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Autores principales:	Kristóf Hegedüs, Csaba Fehér, István Jalsovszky, Zoltán Kristóf, János Rohonczy, Elemér Vass, Attila Farkas, Tamás Csizmadia, Gernot Friedbacher, Peter Hantz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8245d7251a81429a8ee17395353fef2e
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spelling	oai:doaj.org-article:8245d7251a81429a8ee17395353fef2e2021-12-02T15:36:13ZFacile isolation and analysis of sporopollenin exine from bee pollen10.1038/s41598-021-87619-82045-2322https://doaj.org/article/8245d7251a81429a8ee17395353fef2e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87619-8https://doaj.org/toc/2045-2322Abstract We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and aromatic constituents. Purified exine capsules were coated with functionalized fluorophores. The procedures presented in this paper could contribute to further spread of the applications of this hollow, and chemically highly resistant material.Kristóf HegedüsCsaba FehérIstván JalsovszkyZoltán KristófJános RohonczyElemér VassAttila FarkasTamás CsizmadiaGernot FriedbacherPeter HantzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristóf Hegedüs Csaba Fehér István Jalsovszky Zoltán Kristóf János Rohonczy Elemér Vass Attila Farkas Tamás Csizmadia Gernot Friedbacher Peter Hantz Facile isolation and analysis of sporopollenin exine from bee pollen
description	Abstract We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and aromatic constituents. Purified exine capsules were coated with functionalized fluorophores. The procedures presented in this paper could contribute to further spread of the applications of this hollow, and chemically highly resistant material.
format	article
author	Kristóf Hegedüs Csaba Fehér István Jalsovszky Zoltán Kristóf János Rohonczy Elemér Vass Attila Farkas Tamás Csizmadia Gernot Friedbacher Peter Hantz
author_facet	Kristóf Hegedüs Csaba Fehér István Jalsovszky Zoltán Kristóf János Rohonczy Elemér Vass Attila Farkas Tamás Csizmadia Gernot Friedbacher Peter Hantz
author_sort	Kristóf Hegedüs
title	Facile isolation and analysis of sporopollenin exine from bee pollen
title_short	Facile isolation and analysis of sporopollenin exine from bee pollen
title_full	Facile isolation and analysis of sporopollenin exine from bee pollen
title_fullStr	Facile isolation and analysis of sporopollenin exine from bee pollen
title_full_unstemmed	Facile isolation and analysis of sporopollenin exine from bee pollen
title_sort	facile isolation and analysis of sporopollenin exine from bee pollen
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8245d7251a81429a8ee17395353fef2e
work_keys_str_mv	AT kristofhegedus facileisolationandanalysisofsporopolleninexinefrombeepollen AT csabafeher facileisolationandanalysisofsporopolleninexinefrombeepollen AT istvanjalsovszky facileisolationandanalysisofsporopolleninexinefrombeepollen AT zoltankristof facileisolationandanalysisofsporopolleninexinefrombeepollen AT janosrohonczy facileisolationandanalysisofsporopolleninexinefrombeepollen AT elemervass facileisolationandanalysisofsporopolleninexinefrombeepollen AT attilafarkas facileisolationandanalysisofsporopolleninexinefrombeepollen AT tamascsizmadia facileisolationandanalysisofsporopolleninexinefrombeepollen AT gernotfriedbacher facileisolationandanalysisofsporopolleninexinefrombeepollen AT peterhantz facileisolationandanalysisofsporopolleninexinefrombeepollen
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Facile isolation and analysis of sporopollenin exine from bee pollen

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