Phosphate (Pi) Starvation Up-Regulated GmCSN5A/B Participates in Anthocyanin Synthesis in Soybean (Glycine max) Dependent on Pi Availability

Phosphorus (P) is an essential macronutrient for plant growth and development. Among adaptive strategies of plants to P deficiency, increased anthocyanin accumulation is widely observed in plants, which is tightly regulated by a set of genes at transcription levels. However, it remains unclear wheth...

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Autores principales:	Xiaohui Mo, Mengke Zhang, Zeyu Zhang, Xing Lu, Cuiyue Liang, Jiang Tian
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	phosphorus deficiency soybean <i>GmCSN5A/B</i> anthocyanin synthesis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/824776fca68f4740adc56e0f31281da4
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spelling	oai:doaj.org-article:824776fca68f4740adc56e0f31281da42021-11-25T17:55:37ZPhosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability10.3390/ijms2222123481422-00671661-6596https://doaj.org/article/824776fca68f4740adc56e0f31281da42021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12348https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Phosphorus (P) is an essential macronutrient for plant growth and development. Among adaptive strategies of plants to P deficiency, increased anthocyanin accumulation is widely observed in plants, which is tightly regulated by a set of genes at transcription levels. However, it remains unclear whether other key regulators might control anthocyanin synthesis through protein modification under P-deficient conditions. In the study, phosphate (Pi) starvation led to anthocyanin accumulations in soybean (<i>Glycine max</i>) leaves, accompanied with increased transcripts of a group of genes involved in anthocyanin synthesis. Meanwhile, transcripts of <i>GmCSN5A</i>/<i>B</i>, two members of the <i>COP9 signalosome subunit 5</i> (<i>CSN5</i>) family, were up-regulated in both young and old soybean leaves by Pi starvation. Furthermore, overexpressing <i>GmCSN5A</i> and <i>GmCSN5B</i> in <i>Arabidopsis thaliana</i> significantly resulted in anthocyanin accumulations in shoots, accompanied with increased transcripts of gene functions in anthocyanin synthesis including <i>AtPAL</i>, <i>AtCHS</i>, <i>AtF3H</i>, <i>AtF3</i>′<i>H</i>, <i>AtDFR</i>, <i>AtANS</i>, and <i>AtUF3GT</i> only under P-deficient conditions. Taken together, these results strongly suggest that P deficiency leads to increased anthocyanin synthesis through enhancing expression levels of genes involved in anthocyanin synthesis, which could be regulated by GmCSN5A and GmCSN5B.Xiaohui MoMengke ZhangZeyu ZhangXing LuCuiyue LiangJiang TianMDPI AGarticlephosphorus deficiencysoybean<i>GmCSN5A/B</i>anthocyanin synthesisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12348, p 12348 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phosphorus deficiency soybean <i>GmCSN5A/B</i> anthocyanin synthesis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	phosphorus deficiency soybean <i>GmCSN5A/B</i> anthocyanin synthesis Biology (General) QH301-705.5 Chemistry QD1-999 Xiaohui Mo Mengke Zhang Zeyu Zhang Xing Lu Cuiyue Liang Jiang Tian Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
description	Phosphorus (P) is an essential macronutrient for plant growth and development. Among adaptive strategies of plants to P deficiency, increased anthocyanin accumulation is widely observed in plants, which is tightly regulated by a set of genes at transcription levels. However, it remains unclear whether other key regulators might control anthocyanin synthesis through protein modification under P-deficient conditions. In the study, phosphate (Pi) starvation led to anthocyanin accumulations in soybean (<i>Glycine max</i>) leaves, accompanied with increased transcripts of a group of genes involved in anthocyanin synthesis. Meanwhile, transcripts of <i>GmCSN5A</i>/<i>B</i>, two members of the <i>COP9 signalosome subunit 5</i> (<i>CSN5</i>) family, were up-regulated in both young and old soybean leaves by Pi starvation. Furthermore, overexpressing <i>GmCSN5A</i> and <i>GmCSN5B</i> in <i>Arabidopsis thaliana</i> significantly resulted in anthocyanin accumulations in shoots, accompanied with increased transcripts of gene functions in anthocyanin synthesis including <i>AtPAL</i>, <i>AtCHS</i>, <i>AtF3H</i>, <i>AtF3</i>′<i>H</i>, <i>AtDFR</i>, <i>AtANS</i>, and <i>AtUF3GT</i> only under P-deficient conditions. Taken together, these results strongly suggest that P deficiency leads to increased anthocyanin synthesis through enhancing expression levels of genes involved in anthocyanin synthesis, which could be regulated by GmCSN5A and GmCSN5B.
format	article
author	Xiaohui Mo Mengke Zhang Zeyu Zhang Xing Lu Cuiyue Liang Jiang Tian
author_facet	Xiaohui Mo Mengke Zhang Zeyu Zhang Xing Lu Cuiyue Liang Jiang Tian
author_sort	Xiaohui Mo
title	Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
title_short	Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
title_full	Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
title_fullStr	Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
title_full_unstemmed	Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability
title_sort	phosphate (pi) starvation up-regulated <i>gmcsn5a</i>/<i>b</i> participates in anthocyanin synthesis in soybean (<i>glycine max</i>) dependent on pi availability
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/824776fca68f4740adc56e0f31281da4
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Phosphate (Pi) Starvation Up-Regulated <i>GmCSN5A</i>/<i>B</i> Participates in Anthocyanin Synthesis in Soybean (<i>Glycine max</i>) Dependent on Pi Availability

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