Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99

Abstract The aim of this study was to investigate the effects of dietary supplementation with two alternatives to antibiotics (Candida tropicalis and mulberry leaf flavonoids) on intestinal microbiota of preweaned calves challenged with Escherichia coli K99. Sixty Holstein calves were randomly assig...

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Autores principales:	Yanliang Bi, Chuntao Yang, Qiyu Diao, Yan Tu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/824a006fe3bb4c28ade797cebc790186
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spelling	oai:doaj.org-article:824a006fe3bb4c28ade797cebc7901862021-12-02T11:40:34ZEffects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K9910.1038/s41598-017-05376-z2045-2322https://doaj.org/article/824a006fe3bb4c28ade797cebc7901862017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05376-zhttps://doaj.org/toc/2045-2322Abstract The aim of this study was to investigate the effects of dietary supplementation with two alternatives to antibiotics (Candida tropicalis and mulberry leaf flavonoids) on intestinal microbiota of preweaned calves challenged with Escherichia coli K99. Sixty Holstein calves were randomly assigned to 5 treatments: fed a basal diet (N-CON); fed a basal diet and challenged with E.coli K99 (P-CON); fed a basal diet supplemented with C.tropicalis (CT), mulberry leaf flavonoids (MLF), and the combination of the two additives (CM), respectively, and challenged with E.coli K99. The MLF and CM groups had significantly higher average daily grain and feed efficiency, and significantly lower fecal scores compared with the P-CON group after E. coli K99 challenge. The supplementation groups increased the relative abundance, at the phylum level, of Bacteroidetes and Proteobacteria, whereas at the genus level, they increased the relative abundance of Prevotella, Lactobacillus, and Enterococcus. Quantitative PCR revealed that the CT, MLF, and CM groups had significantly lower copy numbers of E.coli K99 compared with the P-CON group. The CT, MLF, and CM treatments reduce days of diarrhea, improve intestinal health, and beneficially manipulate the intestinal microbiota in preweaned calves.Yanliang BiChuntao YangQiyu DiaoYan TuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanliang Bi Chuntao Yang Qiyu Diao Yan Tu Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99
description	Abstract The aim of this study was to investigate the effects of dietary supplementation with two alternatives to antibiotics (Candida tropicalis and mulberry leaf flavonoids) on intestinal microbiota of preweaned calves challenged with Escherichia coli K99. Sixty Holstein calves were randomly assigned to 5 treatments: fed a basal diet (N-CON); fed a basal diet and challenged with E.coli K99 (P-CON); fed a basal diet supplemented with C.tropicalis (CT), mulberry leaf flavonoids (MLF), and the combination of the two additives (CM), respectively, and challenged with E.coli K99. The MLF and CM groups had significantly higher average daily grain and feed efficiency, and significantly lower fecal scores compared with the P-CON group after E. coli K99 challenge. The supplementation groups increased the relative abundance, at the phylum level, of Bacteroidetes and Proteobacteria, whereas at the genus level, they increased the relative abundance of Prevotella, Lactobacillus, and Enterococcus. Quantitative PCR revealed that the CT, MLF, and CM groups had significantly lower copy numbers of E.coli K99 compared with the P-CON group. The CT, MLF, and CM treatments reduce days of diarrhea, improve intestinal health, and beneficially manipulate the intestinal microbiota in preweaned calves.
format	article
author	Yanliang Bi Chuntao Yang Qiyu Diao Yan Tu
author_facet	Yanliang Bi Chuntao Yang Qiyu Diao Yan Tu
author_sort	Yanliang Bi
title	Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99
title_short	Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99
title_full	Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99
title_fullStr	Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99
title_full_unstemmed	Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99
title_sort	effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with escherichia coli k99
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/824a006fe3bb4c28ade797cebc790186
work_keys_str_mv	AT yanliangbi effectsofdietarysupplementationwithtwoalternativestoantibioticsonintestinalmicrobiotaofpreweanedcalveschallengedwithescherichiacolik99 AT chuntaoyang effectsofdietarysupplementationwithtwoalternativestoantibioticsonintestinalmicrobiotaofpreweanedcalveschallengedwithescherichiacolik99 AT qiyudiao effectsofdietarysupplementationwithtwoalternativestoantibioticsonintestinalmicrobiotaofpreweanedcalveschallengedwithescherichiacolik99 AT yantu effectsofdietarysupplementationwithtwoalternativestoantibioticsonintestinalmicrobiotaofpreweanedcalveschallengedwithescherichiacolik99
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Effects of dietary supplementation with two alternatives to antibiotics on intestinal microbiota of preweaned calves challenged with Escherichia coli K99

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