Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great...

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Autores principales:	Flavia Soledad Darqui, Laura Mabel Radonic, Valeria Cecilia Beracochea, H. Esteban Hopp, Marisa López Bilbao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	transgenesis sunflower lettuce model species Asteraceae Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/824d95050e3f41e3b5d358043d6bc031
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spelling	oai:doaj.org-article:824d95050e3f41e3b5d358043d6bc0312021-12-01T07:58:06ZPeculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce1664-462X10.3389/fpls.2021.767459https://doaj.org/article/824d95050e3f41e3b5d358043d6bc0312021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.767459/fullhttps://doaj.org/toc/1664-462XThe Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.Flavia Soledad DarquiLaura Mabel RadonicValeria Cecilia BeracocheaH. Esteban HoppH. Esteban HoppMarisa López BilbaoFrontiers Media S.A.articletransgenesissunflowerlettucemodel speciesAsteraceaePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	transgenesis sunflower lettuce model species Asteraceae Plant culture SB1-1110
spellingShingle	transgenesis sunflower lettuce model species Asteraceae Plant culture SB1-1110 Flavia Soledad Darqui Laura Mabel Radonic Valeria Cecilia Beracochea H. Esteban Hopp H. Esteban Hopp Marisa López Bilbao Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
description	The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.
format	article
author	Flavia Soledad Darqui Laura Mabel Radonic Valeria Cecilia Beracochea H. Esteban Hopp H. Esteban Hopp Marisa López Bilbao
author_facet	Flavia Soledad Darqui Laura Mabel Radonic Valeria Cecilia Beracochea H. Esteban Hopp H. Esteban Hopp Marisa López Bilbao
author_sort	Flavia Soledad Darqui
title	Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_short	Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_full	Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_fullStr	Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_full_unstemmed	Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_sort	peculiarities of the transformation of asteraceae family species: the cases of sunflower and lettuce
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/824d95050e3f41e3b5d358043d6bc031
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_version_	1718405422778417152

Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

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