Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.

<h4>Background</h4>Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is medi...

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Autores principales:	Athos Silva de Oliveira, Fernando Lucas Melo, Alice Kazuko Inoue-Nagata, Tatsuya Nagata, Elliot Watanabe Kitajima, Renato Oliveira Resende
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8252758f52ad424ea7364bca1ea92975
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spelling	oai:doaj.org-article:8252758f52ad424ea7364bca1ea929752021-11-18T07:15:56ZCharacterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.1932-620310.1371/journal.pone.0038634https://doaj.org/article/8252758f52ad424ea7364bca1ea929752012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22715400/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is mediated by thrips in a circulative-propagative manner. For new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history.<h4>Methodology/principal findings</h4>Biological (host range and symptomatology), serological, and molecular (S and M RNA sequencing and evolutionary studies) experiments were performed to characterize and differentiate a new tospovirus species, Bean necrotic mosaic virus (BeNMV), which naturally infects common beans in Brazil. Based upon the results, BeNMV can be classified as a novel species and, together with Soybean vein necrosis-associated virus (SVNaV), they represent members of a new evolutionary lineage within the genus Tospovirus. CONCLUSION/SIGNIFICANCES: Taken together, these evidences suggest that two divergent lineages of tospoviruses are circulating in the American continent and, based on the main clades diversity (American and Eurasian lineages), new tospovirus species related to the BeNMV-SVNaV clade remain to be discovered. This possible greater diversity of tospoviruses may be reflected in a higher number of crops as natural hosts, increasing the economic impact on agriculture. This idea also is supported since BeNMV and SVNaV were discovered naturally infecting atypical hosts (common bean and soybean, respectively), indicating, in this case, a preference for leguminous species. Further studies, for instance a survey focusing on crops, specifically of leguminous plants, may reveal a greater tospovirus diversity not only in the Americas (where both viruses were reported), but throughout the world.Athos Silva de OliveiraFernando Lucas MeloAlice Kazuko Inoue-NagataTatsuya NagataElliot Watanabe KitajimaRenato Oliveira ResendePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e38634 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Athos Silva de Oliveira Fernando Lucas Melo Alice Kazuko Inoue-Nagata Tatsuya Nagata Elliot Watanabe Kitajima Renato Oliveira Resende Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.
description	<h4>Background</h4>Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is mediated by thrips in a circulative-propagative manner. For new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history.<h4>Methodology/principal findings</h4>Biological (host range and symptomatology), serological, and molecular (S and M RNA sequencing and evolutionary studies) experiments were performed to characterize and differentiate a new tospovirus species, Bean necrotic mosaic virus (BeNMV), which naturally infects common beans in Brazil. Based upon the results, BeNMV can be classified as a novel species and, together with Soybean vein necrosis-associated virus (SVNaV), they represent members of a new evolutionary lineage within the genus Tospovirus. CONCLUSION/SIGNIFICANCES: Taken together, these evidences suggest that two divergent lineages of tospoviruses are circulating in the American continent and, based on the main clades diversity (American and Eurasian lineages), new tospovirus species related to the BeNMV-SVNaV clade remain to be discovered. This possible greater diversity of tospoviruses may be reflected in a higher number of crops as natural hosts, increasing the economic impact on agriculture. This idea also is supported since BeNMV and SVNaV were discovered naturally infecting atypical hosts (common bean and soybean, respectively), indicating, in this case, a preference for leguminous species. Further studies, for instance a survey focusing on crops, specifically of leguminous plants, may reveal a greater tospovirus diversity not only in the Americas (where both viruses were reported), but throughout the world.
format	article
author	Athos Silva de Oliveira Fernando Lucas Melo Alice Kazuko Inoue-Nagata Tatsuya Nagata Elliot Watanabe Kitajima Renato Oliveira Resende
author_facet	Athos Silva de Oliveira Fernando Lucas Melo Alice Kazuko Inoue-Nagata Tatsuya Nagata Elliot Watanabe Kitajima Renato Oliveira Resende
author_sort	Athos Silva de Oliveira
title	Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.
title_short	Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.
title_full	Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.
title_fullStr	Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.
title_full_unstemmed	Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.
title_sort	characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the genus tospovirus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8252758f52ad424ea7364bca1ea92975
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Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.

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