Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells

Stephany Y Tzeng, Peter H Yang, Warren L Grayson, Jordan J GreenDepartment of Biomedical Engineering and the Institute for NanoBioTechnology, Johns Hopkins University School of Medicine, Baltimore, MD, USAAbstract: Biodegradable poly(ester amine) (PEA)-based and poly(amido amine) (PAA)-based nanopar...

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Autores principales:	Tzeng SY, Yang PH, Grayson WL, Green JJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/825379abe714474b8586da15a1816ff7
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spelling	oai:doaj.org-article:825379abe714474b8586da15a1816ff72021-12-02T02:48:32ZSynthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells1176-91141178-2013https://doaj.org/article/825379abe714474b8586da15a1816ff72011-12-01T00:00:00Zhttp://www.dovepress.com/synthetic-polyester-amine-and-polyamido-amine-nanoparticles-for-effici-a8862https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Stephany Y Tzeng, Peter H Yang, Warren L Grayson, Jordan J GreenDepartment of Biomedical Engineering and the Institute for NanoBioTechnology, Johns Hopkins University School of Medicine, Baltimore, MD, USAAbstract: Biodegradable poly(ester amine) (PEA)-based and poly(amido amine) (PAA)-based nanoparticles were developed for efficient in vitro siRNA delivery to human umbilical vein endothelial cells (HUVECs). They were screened, characterized, and compared with traditionally studied DNA-containing particles. Several of the polymeric nanoparticles tested were found to be effective for delivering functional siRNA to green fluorescent protein (GFP) + HUVECs, achieving 60%&ndash;75% GFP knockdown while maintaining high viability. While PEAs have been used previously to form polyplexes or nanoparticles for DNA delivery, highly effective siRNA delivery in hard-to-transfect human cell types has not been previously reported. PEAs and linear nondendrimeric PAAs were also found to be effective for DNA delivery to HUVECs using GFP-encoding plasmid DNA (up to 50%&ndash;60% transfection efficiency). PEAs and PAAs can be separated into groups that form polymeric nanoparticles effective for siRNA delivery, for DNA delivery, or for both.Keywords: nanoparticles, siRNA, DNA, gene delivery, human umbilical vein endothelial cellsTzeng SYYang PHGrayson WLGreen JJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3309-3322 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tzeng SY Yang PH Grayson WL Green JJ Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
description	Stephany Y Tzeng, Peter H Yang, Warren L Grayson, Jordan J GreenDepartment of Biomedical Engineering and the Institute for NanoBioTechnology, Johns Hopkins University School of Medicine, Baltimore, MD, USAAbstract: Biodegradable poly(ester amine) (PEA)-based and poly(amido amine) (PAA)-based nanoparticles were developed for efficient in vitro siRNA delivery to human umbilical vein endothelial cells (HUVECs). They were screened, characterized, and compared with traditionally studied DNA-containing particles. Several of the polymeric nanoparticles tested were found to be effective for delivering functional siRNA to green fluorescent protein (GFP) + HUVECs, achieving 60%&ndash;75% GFP knockdown while maintaining high viability. While PEAs have been used previously to form polyplexes or nanoparticles for DNA delivery, highly effective siRNA delivery in hard-to-transfect human cell types has not been previously reported. PEAs and linear nondendrimeric PAAs were also found to be effective for DNA delivery to HUVECs using GFP-encoding plasmid DNA (up to 50%&ndash;60% transfection efficiency). PEAs and PAAs can be separated into groups that form polymeric nanoparticles effective for siRNA delivery, for DNA delivery, or for both.Keywords: nanoparticles, siRNA, DNA, gene delivery, human umbilical vein endothelial cells
format	article
author	Tzeng SY Yang PH Grayson WL Green JJ
author_facet	Tzeng SY Yang PH Grayson WL Green JJ
author_sort	Tzeng SY
title	Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
title_short	Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
title_full	Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
title_fullStr	Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
title_full_unstemmed	Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
title_sort	synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient dna and sirna delivery to human endothelial cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/825379abe714474b8586da15a1816ff7
work_keys_str_mv	AT tzengsy syntheticpolyesteramineandpolyamidoaminenanoparticlesforefficientdnaandsirnadeliverytohumanendothelialcells AT yangph syntheticpolyesteramineandpolyamidoaminenanoparticlesforefficientdnaandsirnadeliverytohumanendothelialcells AT graysonwl syntheticpolyesteramineandpolyamidoaminenanoparticlesforefficientdnaandsirnadeliverytohumanendothelialcells AT greenjj syntheticpolyesteramineandpolyamidoaminenanoparticlesforefficientdnaandsirnadeliverytohumanendothelialcells
_version_	1718402110298521600

Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells

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