3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes

Van der Wee et al. establish a new colloidal crystal standard sample for the alignment and calibration of confocal and stimulated emission depletion microscopy. The sample is adjustable for different NA objectives and microscopy techniques and additionally can be used to measure point spread functio...

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Autores principales:	Ernest B. van der Wee, Jantina Fokkema, Chris L. Kennedy, Marc del Pozo, D. A. Matthijs de Winter, Peter N. A. Speets, Hans C. Gerritsen, Alfons van Blaaderen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/82555801a40a453a9af8b364abf116fb
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spelling	oai:doaj.org-article:82555801a40a453a9af8b364abf116fb2021-12-02T16:17:27Z3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes10.1038/s42003-021-02432-32399-3642https://doaj.org/article/82555801a40a453a9af8b364abf116fb2021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02432-3https://doaj.org/toc/2399-3642Van der Wee et al. establish a new colloidal crystal standard sample for the alignment and calibration of confocal and stimulated emission depletion microscopy. The sample is adjustable for different NA objectives and microscopy techniques and additionally can be used to measure point spread functions and evaluate refractive index mismatches as a function of depth quantitatively.Ernest B. van der WeeJantina FokkemaChris L. KennedyMarc del PozoD. A. Matthijs de WinterPeter N. A. SpeetsHans C. GerritsenAlfons van BlaaderenNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ernest B. van der Wee Jantina Fokkema Chris L. Kennedy Marc del Pozo D. A. Matthijs de Winter Peter N. A. Speets Hans C. Gerritsen Alfons van Blaaderen 3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
description	Van der Wee et al. establish a new colloidal crystal standard sample for the alignment and calibration of confocal and stimulated emission depletion microscopy. The sample is adjustable for different NA objectives and microscopy techniques and additionally can be used to measure point spread functions and evaluate refractive index mismatches as a function of depth quantitatively.
format	article
author	Ernest B. van der Wee Jantina Fokkema Chris L. Kennedy Marc del Pozo D. A. Matthijs de Winter Peter N. A. Speets Hans C. Gerritsen Alfons van Blaaderen
author_facet	Ernest B. van der Wee Jantina Fokkema Chris L. Kennedy Marc del Pozo D. A. Matthijs de Winter Peter N. A. Speets Hans C. Gerritsen Alfons van Blaaderen
author_sort	Ernest B. van der Wee
title	3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
title_short	3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
title_full	3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
title_fullStr	3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
title_full_unstemmed	3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
title_sort	3d test sample for the calibration and quality control of stimulated emission depletion (sted) and confocal microscopes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/82555801a40a453a9af8b364abf116fb
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3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes

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