Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)

Background: Enterococcus is a part of normal gastrointestinal flora in human body. Nevertheless, antibiotic-resistant Enterococcus (ARE) is considered a key factor in nosocomial infections which result in a considerable increase in the rate of patient death due to referring of numerous patients to h...

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Autores principales:	Rahimeh Khavari, Reza Massudi, Afsaneh Karmostaji, Neda Soleimani, Pantea Ashkeshi, Mohammad Rezaei-Pandari
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	enterococci drug resistance-bacterial nosocomial infections bactericidal effect diode laser Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/8256bd3285974c83b9783a9530e929b2
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spelling	oai:doaj.org-article:8256bd3285974c83b9783a9530e929b22021-11-18T06:56:58ZAntibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)2345-33622322-586610.34172/ijep.2020.28https://doaj.org/article/8256bd3285974c83b9783a9530e929b22020-12-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4343https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Enterococcus is a part of normal gastrointestinal flora in human body. Nevertheless, antibiotic-resistant Enterococcus (ARE) is considered a key factor in nosocomial infections which result in a considerable increase in the rate of patient death due to referring of numerous patients to health centers annually, or lead to extended disease convalescence. Objective: This study aimed to evaluate the bactericidal effect at 405nm diode at a laser power of 30 mW on ARE viability of clinical infections. Materials and Methods: In the present study, 30 isolates underwent antibiotic susceptibility test (AST) in which sensitivity to piperacillin (100 µg), rifampin (5 µg), and oxacillin (1 µg) were measured based on the Clinical and Laboratory Standards Institute (CLSI) guidelines. Afterwards, ten most resistant isolates were selected and irradiated by a 405 nm diode laser at a power of 30 mW for 180 and 240 seconds. The data were reported statistically as mean ± standard deviation, and the analysis of the data on varied bacteria was performed using ANOVA. The result was evaluated by SPSS software and P value ≤0.05 was interpreted to be significant. Results: Bacterial viability decreased unsteadily to 10 resistant isolates. Moreover, enhancing irradiation time caused a lower viability rate in such a way that the viability of isolate 9 having the lowest viability rate was reduced from 2.94% in 180 seconds to 0.58% in 240 seconds. The result was evaluated by SPSS software and P value was determined to be significant, and P≤0.05 was laser irradiation for either 180 s or 240 s. Conclusion: Following the study results, 405 nm diode laser could be applied as a tool for eliminating clinical ARE, and it was useful for preventing hospital-acquired infections.Rahimeh KhavariReza MassudiAfsaneh KarmostajiNeda SoleimaniPantea AshkeshiMohammad Rezaei-PandariAlborz University of Medical Sciencesarticleenterococci drug resistance-bacterial nosocomial infections bactericidal effect diode laser Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 4, Pp 137-141 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	enterococci drug resistance-bacterial nosocomial infections bactericidal effect diode laser Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	enterococci drug resistance-bacterial nosocomial infections bactericidal effect diode laser Diseases of the digestive system. Gastroenterology RC799-869 Rahimeh Khavari Reza Massudi Afsaneh Karmostaji Neda Soleimani Pantea Ashkeshi Mohammad Rezaei-Pandari Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
description	Background: Enterococcus is a part of normal gastrointestinal flora in human body. Nevertheless, antibiotic-resistant Enterococcus (ARE) is considered a key factor in nosocomial infections which result in a considerable increase in the rate of patient death due to referring of numerous patients to health centers annually, or lead to extended disease convalescence. Objective: This study aimed to evaluate the bactericidal effect at 405nm diode at a laser power of 30 mW on ARE viability of clinical infections. Materials and Methods: In the present study, 30 isolates underwent antibiotic susceptibility test (AST) in which sensitivity to piperacillin (100 µg), rifampin (5 µg), and oxacillin (1 µg) were measured based on the Clinical and Laboratory Standards Institute (CLSI) guidelines. Afterwards, ten most resistant isolates were selected and irradiated by a 405 nm diode laser at a power of 30 mW for 180 and 240 seconds. The data were reported statistically as mean ± standard deviation, and the analysis of the data on varied bacteria was performed using ANOVA. The result was evaluated by SPSS software and P value ≤0.05 was interpreted to be significant. Results: Bacterial viability decreased unsteadily to 10 resistant isolates. Moreover, enhancing irradiation time caused a lower viability rate in such a way that the viability of isolate 9 having the lowest viability rate was reduced from 2.94% in 180 seconds to 0.58% in 240 seconds. The result was evaluated by SPSS software and P value was determined to be significant, and P≤0.05 was laser irradiation for either 180 s or 240 s. Conclusion: Following the study results, 405 nm diode laser could be applied as a tool for eliminating clinical ARE, and it was useful for preventing hospital-acquired infections.
format	article
author	Rahimeh Khavari Reza Massudi Afsaneh Karmostaji Neda Soleimani Pantea Ashkeshi Mohammad Rezaei-Pandari
author_facet	Rahimeh Khavari Reza Massudi Afsaneh Karmostaji Neda Soleimani Pantea Ashkeshi Mohammad Rezaei-Pandari
author_sort	Rahimeh Khavari
title	Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
title_short	Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
title_full	Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
title_fullStr	Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
title_full_unstemmed	Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)
title_sort	antibacterial effect of low-level laser (diode 405 nm) on antibiotic-resistant enterococci clinical isolates (in vitro)
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/8256bd3285974c83b9783a9530e929b2
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Antibacterial Effect of Low-Level Laser (Diode 405 nm) on Antibiotic-Resistant Enterococci Clinical Isolates (In Vitro)

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