Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.

Biofilms are prevalent in diseases caused by Pseudomonas aeruginosa, an opportunistic and nosocomial pathogen. By a proteomic approach, we previously identified a hypothetical protein of P. aeruginosa (coded by the gene pA3731) that was accumulated by biofilm cells. We report here that a Delta pA373...

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Autores principales:	Camille Macé, Damien Seyer, Chanez Chemani, Pascal Cosette, Patrick Di-Martino, Benoit Guery, Alain Filloux, Marc Fontaine, Virginie Molle, Guy-Alain Junter, Thierry Jouenne
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8259a20f4bb64d798182799e5f6f8893
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spelling	oai:doaj.org-article:8259a20f4bb64d798182799e5f6f88932021-11-25T06:18:16ZIdentification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.1932-620310.1371/journal.pone.0003897https://doaj.org/article/8259a20f4bb64d798182799e5f6f88932008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19065261/?tool=EBIhttps://doaj.org/toc/1932-6203Biofilms are prevalent in diseases caused by Pseudomonas aeruginosa, an opportunistic and nosocomial pathogen. By a proteomic approach, we previously identified a hypothetical protein of P. aeruginosa (coded by the gene pA3731) that was accumulated by biofilm cells. We report here that a Delta pA3731 mutant is highly biofilm-defective as compared with the wild-type strain. Using a mouse model of lung infection, we show that the mutation also induces a defect in bacterial growth during the acute phase of infection and an attenuation of the virulence. The pA3731 gene is found to control positively the ability to swarm and to produce extracellular rhamnolipids, and belongs to a cluster of 4 genes (pA3729-pA3732) not previously described in P. aeruginosa. Though the protein PA3731 has a predicted secondary structure similar to that of the Phage Shock Protein, some obvious differences are observed compared to already described psp systems, e.g., this unknown cluster is monocistronic and no homology is found between the other proteins constituting this locus and psp proteins. As E. coli PspA, the amount of the protein PA3731 is enlarged by an osmotic shock, however, not affected by a heat shock. We consequently named this locus bac for biofilm-associated cluster.Camille MacéDamien SeyerChanez ChemaniPascal CosettePatrick Di-MartinoBenoit GueryAlain FillouxMarc FontaineVirginie MolleGuy-Alain JunterThierry JouennePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e3897 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camille Macé Damien Seyer Chanez Chemani Pascal Cosette Patrick Di-Martino Benoit Guery Alain Filloux Marc Fontaine Virginie Molle Guy-Alain Junter Thierry Jouenne Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.
description	Biofilms are prevalent in diseases caused by Pseudomonas aeruginosa, an opportunistic and nosocomial pathogen. By a proteomic approach, we previously identified a hypothetical protein of P. aeruginosa (coded by the gene pA3731) that was accumulated by biofilm cells. We report here that a Delta pA3731 mutant is highly biofilm-defective as compared with the wild-type strain. Using a mouse model of lung infection, we show that the mutation also induces a defect in bacterial growth during the acute phase of infection and an attenuation of the virulence. The pA3731 gene is found to control positively the ability to swarm and to produce extracellular rhamnolipids, and belongs to a cluster of 4 genes (pA3729-pA3732) not previously described in P. aeruginosa. Though the protein PA3731 has a predicted secondary structure similar to that of the Phage Shock Protein, some obvious differences are observed compared to already described psp systems, e.g., this unknown cluster is monocistronic and no homology is found between the other proteins constituting this locus and psp proteins. As E. coli PspA, the amount of the protein PA3731 is enlarged by an osmotic shock, however, not affected by a heat shock. We consequently named this locus bac for biofilm-associated cluster.
format	article
author	Camille Macé Damien Seyer Chanez Chemani Pascal Cosette Patrick Di-Martino Benoit Guery Alain Filloux Marc Fontaine Virginie Molle Guy-Alain Junter Thierry Jouenne
author_facet	Camille Macé Damien Seyer Chanez Chemani Pascal Cosette Patrick Di-Martino Benoit Guery Alain Filloux Marc Fontaine Virginie Molle Guy-Alain Junter Thierry Jouenne
author_sort	Camille Macé
title	Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.
title_short	Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.
title_full	Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.
title_fullStr	Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.
title_full_unstemmed	Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.
title_sort	identification of biofilm-associated cluster (bac) in pseudomonas aeruginosa involved in biofilm formation and virulence.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/8259a20f4bb64d798182799e5f6f8893
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Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence.

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