Hessian fly-associated bacteria: transmission, essentiality, and composition.

Plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. Gall midges are one of the largest groups of insects that manipulate host plants extensively. Hessian fly (HF, Mayetiola destructor) is an important pest of wheat and a model system for...

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Autores principales:	Raman Bansal, Scot Hulbert, Brandi Schemerhorn, John C Reese, R Jeff Whitworth, Jeffrey J Stuart, Ming-Shun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/825af39afdfa48888538e2082460ae79
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spelling	oai:doaj.org-article:825af39afdfa48888538e2082460ae792021-11-18T06:47:54ZHessian fly-associated bacteria: transmission, essentiality, and composition.1932-620310.1371/journal.pone.0023170https://doaj.org/article/825af39afdfa48888538e2082460ae792011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21858016/?tool=EBIhttps://doaj.org/toc/1932-6203Plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. Gall midges are one of the largest groups of insects that manipulate host plants extensively. Hessian fly (HF, Mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. To examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with HF was performed for the first time. Diverse bacteria were found in different developmental HF stages. Fluorescent in situ hybridization detected a bacteriocyte-like structure in developing eggs. Bacterial DNA was also detected in eggs by PCR using primers targeted to different bacterial groups. These results indicated that HF hosted different types of bacteria that were maternally transmitted to the next generation. Eliminating bacteria from the insect with antibiotics resulted in high mortality of HF larvae, indicating that symbiotic bacteria are essential for the insect to survive on wheat seedlings. A preliminary survey identified various types of bacteria associated with different HF stages, including the genera Enterobacter, Pantoea, Stenotrophomonas, Pseudomonas, Bacillus, Ochrobactrum, Acinetobacter, Alcaligenes, Nitrosomonas, Arcanobacterium, Microbacterium, Paenibacillus, and Klebsiella. Similar bacteria were also found specifically in HF-infested susceptible wheat, suggesting that HF larvae had either transmitted bacteria into plant tissue or brought secondary infection of bacteria to the wheat host. The bacteria associated with wheat seedlings may play an essential role in the wheat-HF interaction.Raman BansalScot HulbertBrandi SchemerhornJohn C ReeseR Jeff WhitworthJeffrey J StuartMing-Shun ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23170 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raman Bansal Scot Hulbert Brandi Schemerhorn John C Reese R Jeff Whitworth Jeffrey J Stuart Ming-Shun Chen Hessian fly-associated bacteria: transmission, essentiality, and composition.
description	Plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. Gall midges are one of the largest groups of insects that manipulate host plants extensively. Hessian fly (HF, Mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. To examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with HF was performed for the first time. Diverse bacteria were found in different developmental HF stages. Fluorescent in situ hybridization detected a bacteriocyte-like structure in developing eggs. Bacterial DNA was also detected in eggs by PCR using primers targeted to different bacterial groups. These results indicated that HF hosted different types of bacteria that were maternally transmitted to the next generation. Eliminating bacteria from the insect with antibiotics resulted in high mortality of HF larvae, indicating that symbiotic bacteria are essential for the insect to survive on wheat seedlings. A preliminary survey identified various types of bacteria associated with different HF stages, including the genera Enterobacter, Pantoea, Stenotrophomonas, Pseudomonas, Bacillus, Ochrobactrum, Acinetobacter, Alcaligenes, Nitrosomonas, Arcanobacterium, Microbacterium, Paenibacillus, and Klebsiella. Similar bacteria were also found specifically in HF-infested susceptible wheat, suggesting that HF larvae had either transmitted bacteria into plant tissue or brought secondary infection of bacteria to the wheat host. The bacteria associated with wheat seedlings may play an essential role in the wheat-HF interaction.
format	article
author	Raman Bansal Scot Hulbert Brandi Schemerhorn John C Reese R Jeff Whitworth Jeffrey J Stuart Ming-Shun Chen
author_facet	Raman Bansal Scot Hulbert Brandi Schemerhorn John C Reese R Jeff Whitworth Jeffrey J Stuart Ming-Shun Chen
author_sort	Raman Bansal
title	Hessian fly-associated bacteria: transmission, essentiality, and composition.
title_short	Hessian fly-associated bacteria: transmission, essentiality, and composition.
title_full	Hessian fly-associated bacteria: transmission, essentiality, and composition.
title_fullStr	Hessian fly-associated bacteria: transmission, essentiality, and composition.
title_full_unstemmed	Hessian fly-associated bacteria: transmission, essentiality, and composition.
title_sort	hessian fly-associated bacteria: transmission, essentiality, and composition.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/825af39afdfa48888538e2082460ae79
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Hessian fly-associated bacteria: transmission, essentiality, and composition.

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