Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage

Abstract Potato (Solanum tuberosum) is an important staple crop worldwide, it has been cultivated in the Andean Altiplano under low-input farming practices at high altitudes and under harsh environment for centuries. We analyzed secondary metabolite (SM) gene diversity encoded in the potato rhizosph...

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Autores principales: Gajender Aleti, Branislav Nikolić, Günter Brader, Ram Vinay Pandey, Livio Antonielli, Stefan Pfeiffer, Andreas Oswald, Angela Sessitsch
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/825bb7f502184db690c5046e98c36851
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spelling oai:doaj.org-article:825bb7f502184db690c5046e98c368512021-12-02T15:06:03ZSecondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage10.1038/s41598-017-02314-x2045-2322https://doaj.org/article/825bb7f502184db690c5046e98c368512017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02314-xhttps://doaj.org/toc/2045-2322Abstract Potato (Solanum tuberosum) is an important staple crop worldwide, it has been cultivated in the Andean Altiplano under low-input farming practices at high altitudes and under harsh environment for centuries. We analyzed secondary metabolite (SM) gene diversity encoded in the potato rhizosphere microbiome during plant growth at three distinct sites located in the Andes at high altitudes by 454-pyrosequencing of non-ribosomal peptide and polyketide biosynthetic genes. Phylogenetic analysis indicated that the majority of rhizosphere SM-encoding sequences differed from previously known sequences and may have distinct ancestors. In particular, actinobacterial methyl-malonyl-CoA transferase and acyl carrier protein from Firmicutes, both involved in the synthesis of SMs, showed widespread distribution of clades which were clearly distinct from sequences deposited in public databases, and only 11% of these sequences could be linked to the production of specific classes of SMs. Although the same cultivar was analyzed, SM gene composition radically differed among plant growth stages and across sites, suggesting a distinct repertoire of SM genes that likely encode diverse SM structures. Also, great diversity of non-ribosomal peptide and polyketide biosynthetic pathways in potato-associated microbiomes in the Andean highlands may represent a rich source of novel natural products.Gajender AletiBranislav NikolićGünter BraderRam Vinay PandeyLivio AntonielliStefan PfeifferAndreas OswaldAngela SessitschNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Gajender Aleti
Branislav Nikolić
Günter Brader
Ram Vinay Pandey
Livio Antonielli
Stefan Pfeiffer
Andreas Oswald
Angela Sessitsch
Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage
description Abstract Potato (Solanum tuberosum) is an important staple crop worldwide, it has been cultivated in the Andean Altiplano under low-input farming practices at high altitudes and under harsh environment for centuries. We analyzed secondary metabolite (SM) gene diversity encoded in the potato rhizosphere microbiome during plant growth at three distinct sites located in the Andes at high altitudes by 454-pyrosequencing of non-ribosomal peptide and polyketide biosynthetic genes. Phylogenetic analysis indicated that the majority of rhizosphere SM-encoding sequences differed from previously known sequences and may have distinct ancestors. In particular, actinobacterial methyl-malonyl-CoA transferase and acyl carrier protein from Firmicutes, both involved in the synthesis of SMs, showed widespread distribution of clades which were clearly distinct from sequences deposited in public databases, and only 11% of these sequences could be linked to the production of specific classes of SMs. Although the same cultivar was analyzed, SM gene composition radically differed among plant growth stages and across sites, suggesting a distinct repertoire of SM genes that likely encode diverse SM structures. Also, great diversity of non-ribosomal peptide and polyketide biosynthetic pathways in potato-associated microbiomes in the Andean highlands may represent a rich source of novel natural products.
format article
author Gajender Aleti
Branislav Nikolić
Günter Brader
Ram Vinay Pandey
Livio Antonielli
Stefan Pfeiffer
Andreas Oswald
Angela Sessitsch
author_facet Gajender Aleti
Branislav Nikolić
Günter Brader
Ram Vinay Pandey
Livio Antonielli
Stefan Pfeiffer
Andreas Oswald
Angela Sessitsch
author_sort Gajender Aleti
title Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage
title_short Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage
title_full Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage
title_fullStr Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage
title_full_unstemmed Secondary metabolite genes encoded by potato rhizosphere microbiomes in the Andean highlands are diverse and vary with sampling site and vegetation stage
title_sort secondary metabolite genes encoded by potato rhizosphere microbiomes in the andean highlands are diverse and vary with sampling site and vegetation stage
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/825bb7f502184db690c5046e98c36851
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