Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.

The human glioma tumor suppressor candidate region 2 gene product, GLTSCR2, also called 'protein interacting with carboxyl terminus 1' (PICT-1), has been implicated in the regulation of two major tumor suppressor proteins, PTEN and p53, and reported to bind the membrane-cytoskeleton regula...

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Autores principales:	Inna Kalt, Ayelet Levy, Tatyana Borodianskiy-Shteinberg, Ronit Sarid
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/826000565b8b4b1cb63438385000a620
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spelling	oai:doaj.org-article:826000565b8b4b1cb63438385000a6202021-11-18T07:29:33ZNucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.1932-620310.1371/journal.pone.0030825https://doaj.org/article/826000565b8b4b1cb63438385000a6202012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22292050/?tool=EBIhttps://doaj.org/toc/1932-6203The human glioma tumor suppressor candidate region 2 gene product, GLTSCR2, also called 'protein interacting with carboxyl terminus 1' (PICT-1), has been implicated in the regulation of two major tumor suppressor proteins, PTEN and p53, and reported to bind the membrane-cytoskeleton regulator of cell signaling, Merlin. PICT-1 is a nucleolar protein, conserved among eukaryotes, and its yeast homolog has been functionally associated with ribosomal RNA processing. By means of confocal microscopy of EGFP and myc-tagged PICT-1 fusion proteins, we delineate that the nucleolar localization of PICT-1 is mediated by two independent nucleolar localization sequences (NoLS). Unlike most NoLSs, these NoLSs are relatively long with flexible boundaries and contain arginine and leucine clusters. In addition, we show that PICT-1 exhibits a nucleolar distribution similar to proteins involved in ribosomal RNA processing, yet does not colocalize precisely with either UBF1 or Fibrillarin under normal or stressed conditions. Identification of the precise location of PICT-1 and the signals that mediate its nucleolar localization is an important step towards advancing our understanding of the demonstrated influence of this protein on cell fate and tumorigenesis.Inna KaltAyelet LevyTatyana Borodianskiy-ShteinbergRonit SaridPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30825 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inna Kalt Ayelet Levy Tatyana Borodianskiy-Shteinberg Ronit Sarid Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
description	The human glioma tumor suppressor candidate region 2 gene product, GLTSCR2, also called 'protein interacting with carboxyl terminus 1' (PICT-1), has been implicated in the regulation of two major tumor suppressor proteins, PTEN and p53, and reported to bind the membrane-cytoskeleton regulator of cell signaling, Merlin. PICT-1 is a nucleolar protein, conserved among eukaryotes, and its yeast homolog has been functionally associated with ribosomal RNA processing. By means of confocal microscopy of EGFP and myc-tagged PICT-1 fusion proteins, we delineate that the nucleolar localization of PICT-1 is mediated by two independent nucleolar localization sequences (NoLS). Unlike most NoLSs, these NoLSs are relatively long with flexible boundaries and contain arginine and leucine clusters. In addition, we show that PICT-1 exhibits a nucleolar distribution similar to proteins involved in ribosomal RNA processing, yet does not colocalize precisely with either UBF1 or Fibrillarin under normal or stressed conditions. Identification of the precise location of PICT-1 and the signals that mediate its nucleolar localization is an important step towards advancing our understanding of the demonstrated influence of this protein on cell fate and tumorigenesis.
format	article
author	Inna Kalt Ayelet Levy Tatyana Borodianskiy-Shteinberg Ronit Sarid
author_facet	Inna Kalt Ayelet Levy Tatyana Borodianskiy-Shteinberg Ronit Sarid
author_sort	Inna Kalt
title	Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
title_short	Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
title_full	Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
title_fullStr	Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
title_full_unstemmed	Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
title_sort	nucleolar localization of gltscr2/pict-1 is mediated by multiple unique nucleolar localization sequences.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/826000565b8b4b1cb63438385000a620
work_keys_str_mv	AT innakalt nucleolarlocalizationofgltscr2pict1ismediatedbymultipleuniquenucleolarlocalizationsequences AT ayeletlevy nucleolarlocalizationofgltscr2pict1ismediatedbymultipleuniquenucleolarlocalizationsequences AT tatyanaborodianskiyshteinberg nucleolarlocalizationofgltscr2pict1ismediatedbymultipleuniquenucleolarlocalizationsequences AT ronitsarid nucleolarlocalizationofgltscr2pict1ismediatedbymultipleuniquenucleolarlocalizationsequences
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Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.

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