A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis)
Herein, we describe a novel bunyavirus, oriental wenrivirus 1 (OWV1), discovered in moribund oriental shrimp (Penaeus chinensis) collected from a farm in China in 2016. Like most bunyaviruses, OWV1 particles were enveloped, spherical- to ovoid-shaped, and 80–115 nm in diameter. However, its genome w...
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2021
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oai:doaj.org-article:82647aef394b4583b9b103ceb1af4dd52021-11-30T18:31:36ZA Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis)1664-302X10.3389/fmicb.2021.751112https://doaj.org/article/82647aef394b4583b9b103ceb1af4dd52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.751112/fullhttps://doaj.org/toc/1664-302XHerein, we describe a novel bunyavirus, oriental wenrivirus 1 (OWV1), discovered in moribund oriental shrimp (Penaeus chinensis) collected from a farm in China in 2016. Like most bunyaviruses, OWV1 particles were enveloped, spherical- to ovoid-shaped, and 80–115 nm in diameter. However, its genome was found to comprise four segments of (-)ssRNA. These included an L RNA segment (6,317 nt) encoding an RNA-directed RNA polymerase (RdRp) of 2,052 aa, an M RNA segment (2,978 nt) encoding a glycoprotein precursor (GPC) of 922 aa, an S1 RNA segment (1,164 nt) encoding a nucleocapsid (N) protein of 243 aa, and an S2 RNA segment (1,382 nt) encoding a putative non-structural (NSs2) protein of 401 aa. All the four OWV1 RNA segments have complementary terminal decanucleotides (5′-ACACAAAGAC and 3′-UGUGUUUCUG) identical to the genomic RNA segments of uukuviruses and similar to those of phleboviruses and tenuiviruses in the Phenuiviridae. Phylogenetic analyses revealed that the RdRp, GPC, and N proteins of OWV1 were closely related to Wēnzhōu shrimp virus 1 (WzSV-1) and Mourilyan virus (MoV) that infect black tiger shrimp (P. monodon). Phylogenetic analyses also suggested that OWV1 could be classified into a second, yet to be established, species of the Wenrivirus genus in the Phenuiviridae. These wenriviruses also clustered with Wenling crustacean virus 7 from shrimps and bunya-like brown spot virus from white-clawed crayfish. Of note there were no homologs of the NSs2 of OWV1 and MoV/WzSV-1 in GenBank, and whether other crustacean phenuiviruses also possess a similar S2 RNA segment warrants further investigation. In addition, we established a TaqMan probe-based reverse-transcription quantitative PCR method for detection of OWV1, and it was detected as 1.17 × 102—1.90 × 107 copies/ng-RNA in gills of 23 out of 32 P. chinensis samples without an obvious gross sign. However, the discovery of OWV1 highlights the expanding genomic diversity of bunyaviruses.Xuan DongTao HuYanbei RenYanbei RenFanzeng MengChen LiQingli ZhangJiayuan ChenJipeng SongJipeng SongRuoyu WangMang ShiJuan LiPeng ZhaoCixiu LiKathy F. J. TangJeff A. CowleyWeifeng ShiJie HuangJie HuangFrontiers Media S.A.articlebunyavirusPenaeus chinensisWenrivirusPhenuiviridaeshrimpMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
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EN |
| topic |
bunyavirus Penaeus chinensis Wenrivirus Phenuiviridae shrimp Microbiology QR1-502 |
| spellingShingle |
bunyavirus Penaeus chinensis Wenrivirus Phenuiviridae shrimp Microbiology QR1-502 Xuan Dong Tao Hu Yanbei Ren Yanbei Ren Fanzeng Meng Chen Li Qingli Zhang Jiayuan Chen Jipeng Song Jipeng Song Ruoyu Wang Mang Shi Juan Li Peng Zhao Cixiu Li Kathy F. J. Tang Jeff A. Cowley Weifeng Shi Jie Huang Jie Huang A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis) |
| description |
Herein, we describe a novel bunyavirus, oriental wenrivirus 1 (OWV1), discovered in moribund oriental shrimp (Penaeus chinensis) collected from a farm in China in 2016. Like most bunyaviruses, OWV1 particles were enveloped, spherical- to ovoid-shaped, and 80–115 nm in diameter. However, its genome was found to comprise four segments of (-)ssRNA. These included an L RNA segment (6,317 nt) encoding an RNA-directed RNA polymerase (RdRp) of 2,052 aa, an M RNA segment (2,978 nt) encoding a glycoprotein precursor (GPC) of 922 aa, an S1 RNA segment (1,164 nt) encoding a nucleocapsid (N) protein of 243 aa, and an S2 RNA segment (1,382 nt) encoding a putative non-structural (NSs2) protein of 401 aa. All the four OWV1 RNA segments have complementary terminal decanucleotides (5′-ACACAAAGAC and 3′-UGUGUUUCUG) identical to the genomic RNA segments of uukuviruses and similar to those of phleboviruses and tenuiviruses in the Phenuiviridae. Phylogenetic analyses revealed that the RdRp, GPC, and N proteins of OWV1 were closely related to Wēnzhōu shrimp virus 1 (WzSV-1) and Mourilyan virus (MoV) that infect black tiger shrimp (P. monodon). Phylogenetic analyses also suggested that OWV1 could be classified into a second, yet to be established, species of the Wenrivirus genus in the Phenuiviridae. These wenriviruses also clustered with Wenling crustacean virus 7 from shrimps and bunya-like brown spot virus from white-clawed crayfish. Of note there were no homologs of the NSs2 of OWV1 and MoV/WzSV-1 in GenBank, and whether other crustacean phenuiviruses also possess a similar S2 RNA segment warrants further investigation. In addition, we established a TaqMan probe-based reverse-transcription quantitative PCR method for detection of OWV1, and it was detected as 1.17 × 102—1.90 × 107 copies/ng-RNA in gills of 23 out of 32 P. chinensis samples without an obvious gross sign. However, the discovery of OWV1 highlights the expanding genomic diversity of bunyaviruses. |
| format |
article |
| author |
Xuan Dong Tao Hu Yanbei Ren Yanbei Ren Fanzeng Meng Chen Li Qingli Zhang Jiayuan Chen Jipeng Song Jipeng Song Ruoyu Wang Mang Shi Juan Li Peng Zhao Cixiu Li Kathy F. J. Tang Jeff A. Cowley Weifeng Shi Jie Huang Jie Huang |
| author_facet |
Xuan Dong Tao Hu Yanbei Ren Yanbei Ren Fanzeng Meng Chen Li Qingli Zhang Jiayuan Chen Jipeng Song Jipeng Song Ruoyu Wang Mang Shi Juan Li Peng Zhao Cixiu Li Kathy F. J. Tang Jeff A. Cowley Weifeng Shi Jie Huang Jie Huang |
| author_sort |
Xuan Dong |
| title |
A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis) |
| title_short |
A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis) |
| title_full |
A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis) |
| title_fullStr |
A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis) |
| title_full_unstemmed |
A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis) |
| title_sort |
novel bunyavirus discovered in oriental shrimp (penaeus chinensis) |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/82647aef394b4583b9b103ceb1af4dd5 |
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