Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds

Medullary thyroid carcinoma contributes to about 3–4% of thyroid cancers and affects C cells rather than follicular cells. Thyroid C cell differentiation from human pluripotent stem cells has not been reported. We report the stepwise differentiation of human embryonic stem cells into thyroid C cell-...

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Autores principales:	Kwaku Dad Abu-Bonsrah, Donald F. Newgreen, Mirella Dottori
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pluripotent stem cells endoderm disease modelling thyroid C cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/82672331be0847ffa9864436aba4d8ba
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spelling	oai:doaj.org-article:82672331be0847ffa9864436aba4d8ba2021-11-25T17:08:40ZDevelopment of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds10.3390/cells101128972073-4409https://doaj.org/article/82672331be0847ffa9864436aba4d8ba2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2897https://doaj.org/toc/2073-4409Medullary thyroid carcinoma contributes to about 3–4% of thyroid cancers and affects C cells rather than follicular cells. Thyroid C cell differentiation from human pluripotent stem cells has not been reported. We report the stepwise differentiation of human embryonic stem cells into thyroid C cell-like cells through definitive endoderm and anterior foregut endoderm and ultimobranchial body-like intermediates in monolayer and 3D Matrigel culture conditions. The protocol involved sequential treatment with interferon/transferrin/selenium/pyruvate, foetal bovine serum, and activin A, then IGF-1 (Insulin-like growth factor 1), on the basis of embryonic thyroid developmental sequence. As well as expressing C cell lineage relative to follicular-lineage markers by qPCR (quantitative polymerase chain reaction) and immunolabelling, these cells by ELISA (enzyme-linked immunoassay) exhibited functional properties in vitro of calcitonin storage and release of calcitonin on calcium challenge. This method will contribute to developmental studies of the human thyroid gland and facilitate in vitro modelling of medullary thyroid carcinoma and provide a valuable platform for drug screening.Kwaku Dad Abu-BonsrahDonald F. NewgreenMirella DottoriMDPI AGarticlepluripotent stem cellsendodermdisease modellingthyroid C cellsBiology (General)QH301-705.5ENCells, Vol 10, Iss 2897, p 2897 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pluripotent stem cells endoderm disease modelling thyroid C cells Biology (General) QH301-705.5
spellingShingle	pluripotent stem cells endoderm disease modelling thyroid C cells Biology (General) QH301-705.5 Kwaku Dad Abu-Bonsrah Donald F. Newgreen Mirella Dottori Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds
description	Medullary thyroid carcinoma contributes to about 3–4% of thyroid cancers and affects C cells rather than follicular cells. Thyroid C cell differentiation from human pluripotent stem cells has not been reported. We report the stepwise differentiation of human embryonic stem cells into thyroid C cell-like cells through definitive endoderm and anterior foregut endoderm and ultimobranchial body-like intermediates in monolayer and 3D Matrigel culture conditions. The protocol involved sequential treatment with interferon/transferrin/selenium/pyruvate, foetal bovine serum, and activin A, then IGF-1 (Insulin-like growth factor 1), on the basis of embryonic thyroid developmental sequence. As well as expressing C cell lineage relative to follicular-lineage markers by qPCR (quantitative polymerase chain reaction) and immunolabelling, these cells by ELISA (enzyme-linked immunoassay) exhibited functional properties in vitro of calcitonin storage and release of calcitonin on calcium challenge. This method will contribute to developmental studies of the human thyroid gland and facilitate in vitro modelling of medullary thyroid carcinoma and provide a valuable platform for drug screening.
format	article
author	Kwaku Dad Abu-Bonsrah Donald F. Newgreen Mirella Dottori
author_facet	Kwaku Dad Abu-Bonsrah Donald F. Newgreen Mirella Dottori
author_sort	Kwaku Dad Abu-Bonsrah
title	Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds
title_short	Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds
title_full	Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds
title_fullStr	Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds
title_full_unstemmed	Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds
title_sort	development of functional thyroid c cell-like cells from human pluripotent cells in 2d and in 3d scaffolds
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/82672331be0847ffa9864436aba4d8ba
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Development of Functional Thyroid C Cell-like Cells from Human Pluripotent Cells in 2D and in 3D Scaffolds

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