Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles

Minhong Jeun,1 Sungwook Park,1,2 Hakho Lee,3 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea; 3Center for Systems...

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Autores principales:	Jeun M, Park S, Lee H, Lee KH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Biosensor NMR Nanoferrite Particles Transverse Relaxation Magnetization On-site detection Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/82694d7543094622a623d4008f9430be
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spelling	oai:doaj.org-article:82694d7543094622a623d4008f9430be2021-12-02T00:46:38ZHighly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles1178-2013https://doaj.org/article/82694d7543094622a623d4008f9430be2016-10-01T00:00:00Zhttps://www.dovepress.com/highly-sensitive-detection-of-protein-biomarkers-via-nuclear-magnetic--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Minhong Jeun,1 Sungwook Park,1,2 Hakho Lee,3 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea; 3Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA Abstract: Magnetic-based biosensors are attractive for on-site detection of biomarkers due to the low magnetic susceptibility of biological samples. Here, we report a highly sensitive magnetic-based biosensing system that is composed of a miniaturized nuclear magnetic resonance (NMR) device and magnetically engineered nanoferrite particles (NFPs). The sensing performance, also identified as the transverse relaxation (R2) rate, of the NMR device is directly related to the magnetic properties of the NFPs. Therefore, we developed magnetically engineered NFPs (MnMg-NFP) and used them as NMR agents to exhibit a significantly improved R2 rate. The magnetization of the MnMg-NFPs was increased by controlling the Mn and Mg cation concentration and distribution during the synthesis process. This modification of the Mn and Mg cation directly contributed to improving the R2 rate. The miniaturized NMR system, combined with the magnetically engineered MnMg-NFPs, successfully detected a small amount of infectious influenza A H1N1 nucleoprotein with high sensitivity and stability. Keywords: biosensor, NMR, nanoferrite particles, transverse relaxation, magnetization, on-site detectionJeun MPark SLee HLee KHDove Medical PressarticleBiosensorNMRNanoferrite ParticlesTransverse RelaxationMagnetizationOn-site detectionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5497-5503 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biosensor NMR Nanoferrite Particles Transverse Relaxation Magnetization On-site detection Medicine (General) R5-920
spellingShingle	Biosensor NMR Nanoferrite Particles Transverse Relaxation Magnetization On-site detection Medicine (General) R5-920 Jeun M Park S Lee H Lee KH Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
description	Minhong Jeun,1 Sungwook Park,1,2 Hakho Lee,3 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea; 3Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA Abstract: Magnetic-based biosensors are attractive for on-site detection of biomarkers due to the low magnetic susceptibility of biological samples. Here, we report a highly sensitive magnetic-based biosensing system that is composed of a miniaturized nuclear magnetic resonance (NMR) device and magnetically engineered nanoferrite particles (NFPs). The sensing performance, also identified as the transverse relaxation (R2) rate, of the NMR device is directly related to the magnetic properties of the NFPs. Therefore, we developed magnetically engineered NFPs (MnMg-NFP) and used them as NMR agents to exhibit a significantly improved R2 rate. The magnetization of the MnMg-NFPs was increased by controlling the Mn and Mg cation concentration and distribution during the synthesis process. This modification of the Mn and Mg cation directly contributed to improving the R2 rate. The miniaturized NMR system, combined with the magnetically engineered MnMg-NFPs, successfully detected a small amount of infectious influenza A H1N1 nucleoprotein with high sensitivity and stability. Keywords: biosensor, NMR, nanoferrite particles, transverse relaxation, magnetization, on-site detection
format	article
author	Jeun M Park S Lee H Lee KH
author_facet	Jeun M Park S Lee H Lee KH
author_sort	Jeun M
title	Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
title_short	Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
title_full	Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
title_fullStr	Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
title_full_unstemmed	Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
title_sort	highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/82694d7543094622a623d4008f9430be
work_keys_str_mv	AT jeunm highlysensitivedetectionofproteinbiomarkersvianuclearmagneticresonancebiosensorwithmagneticallyengineerednanoferriteparticles AT parks highlysensitivedetectionofproteinbiomarkersvianuclearmagneticresonancebiosensorwithmagneticallyengineerednanoferriteparticles AT leeh highlysensitivedetectionofproteinbiomarkersvianuclearmagneticresonancebiosensorwithmagneticallyengineerednanoferriteparticles AT leekh highlysensitivedetectionofproteinbiomarkersvianuclearmagneticresonancebiosensorwithmagneticallyengineerednanoferriteparticles
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Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles

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