Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system

Abstract We studied the molecular epidemiology and mechanism of azole resistance of 164 C. guilliermondii isolates from a nationwide multi-center surveillance program. The isolates were identified by ITS gene sequencing, and the in vitro susceptibility to fluconazole and voriconazole was determined...

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Autores principales:	Jing-Wei Cheng, Kang Liao, Timothy Kudinha, Shu-Ying Yu, Meng Xiao, He Wang, Fanrong Kong, Ying-Chun Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/826a4ef12446401ea5bb978805e0c332
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spelling	oai:doaj.org-article:826a4ef12446401ea5bb978805e0c3322021-12-02T12:30:26ZMolecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system10.1038/s41598-017-01106-72045-2322https://doaj.org/article/826a4ef12446401ea5bb978805e0c3322017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01106-7https://doaj.org/toc/2045-2322Abstract We studied the molecular epidemiology and mechanism of azole resistance of 164 C. guilliermondii isolates from a nationwide multi-center surveillance program. The isolates were identified by ITS gene sequencing, and the in vitro susceptibility to fluconazole and voriconazole was determined by broth microdilution method. The 14-α-demethylase gene ERG11 was amplified and sequenced, and microsatellite analysis was performed to study the genetic relatedness of the isolates. Amongst the 164 C. guilliermondii isolates, 15 (9.1%) and 17 (10.4%) isolates were assigned to be non-wild type (non-WT) to fluconazole and voriconazole, respectively. Sixteen sequence types (STs) were detected by comparing the amino acid sequence polymorphisms of the ERG11 gene. Fifteen isolates of STs 9, 10, 12, 13, 14, 15 and 16, were all assigned to be non-WT to fluconazole and voriconazole. By microsatellite analysis, 40 different genotypes were identified. Thirty-seven isolates from one hospital (Z1) shared the same ERG11 sequence type (ST 2), microsatellite genotype (PU40) and drug resistance pattern. In conclusion, this is the first molecular epidemiology study of C. guilliermondii in China. The rate of non-WT isolates to azoles was high and the accurate contribution of ERG11 gene mutations to azole resistance need be confirmed by further studies.Jing-Wei ChengKang LiaoTimothy KudinhaShu-Ying YuMeng XiaoHe WangFanrong KongYing-Chun XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing-Wei Cheng Kang Liao Timothy Kudinha Shu-Ying Yu Meng Xiao He Wang Fanrong Kong Ying-Chun Xu Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system
description	Abstract We studied the molecular epidemiology and mechanism of azole resistance of 164 C. guilliermondii isolates from a nationwide multi-center surveillance program. The isolates were identified by ITS gene sequencing, and the in vitro susceptibility to fluconazole and voriconazole was determined by broth microdilution method. The 14-α-demethylase gene ERG11 was amplified and sequenced, and microsatellite analysis was performed to study the genetic relatedness of the isolates. Amongst the 164 C. guilliermondii isolates, 15 (9.1%) and 17 (10.4%) isolates were assigned to be non-wild type (non-WT) to fluconazole and voriconazole, respectively. Sixteen sequence types (STs) were detected by comparing the amino acid sequence polymorphisms of the ERG11 gene. Fifteen isolates of STs 9, 10, 12, 13, 14, 15 and 16, were all assigned to be non-WT to fluconazole and voriconazole. By microsatellite analysis, 40 different genotypes were identified. Thirty-seven isolates from one hospital (Z1) shared the same ERG11 sequence type (ST 2), microsatellite genotype (PU40) and drug resistance pattern. In conclusion, this is the first molecular epidemiology study of C. guilliermondii in China. The rate of non-WT isolates to azoles was high and the accurate contribution of ERG11 gene mutations to azole resistance need be confirmed by further studies.
format	article
author	Jing-Wei Cheng Kang Liao Timothy Kudinha Shu-Ying Yu Meng Xiao He Wang Fanrong Kong Ying-Chun Xu
author_facet	Jing-Wei Cheng Kang Liao Timothy Kudinha Shu-Ying Yu Meng Xiao He Wang Fanrong Kong Ying-Chun Xu
author_sort	Jing-Wei Cheng
title	Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system
title_short	Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system
title_full	Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system
title_fullStr	Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system
title_full_unstemmed	Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system
title_sort	molecular epidemiology and azole resistance mechanism study of candida guilliermondii from a chinese surveillance system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/826a4ef12446401ea5bb978805e0c332
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Molecular epidemiology and azole resistance mechanism study of Candida guilliermondii from a Chinese surveillance system

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