Investigating a human pesticide intoxication incident: The importance of robust analytical approaches

A human intoxication incident attributed to pesticide abuse was investigated using cutting-edge analytical methodologies. An LC-ESI-MS/MS method, based on a hybrid solid-phase extraction protocol (hybrid-SPE), was applied for the detection and quantification of several pesticides and metabolites in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kasiotis Konstantinos M., Baira Eirini, Manea-Karga Electra, Nikolopoulou Dimitra, Ganas Konstantinos, Machera Kyriaki
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	pesticides metabolites lc-esi-ms/ms uhplc-hrms biological fluids Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/826f8340625f430ab96760771f200572
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:826f8340625f430ab96760771f200572
record_format	dspace
spelling	oai:doaj.org-article:826f8340625f430ab96760771f2005722021-12-05T14:10:44ZInvestigating a human pesticide intoxication incident: The importance of robust analytical approaches2391-542010.1515/chem-2021-0193https://doaj.org/article/826f8340625f430ab96760771f2005722021-02-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0193https://doaj.org/toc/2391-5420A human intoxication incident attributed to pesticide abuse was investigated using cutting-edge analytical methodologies. An LC-ESI-MS/MS method, based on a hybrid solid-phase extraction protocol (hybrid-SPE), was applied for the detection and quantification of several pesticides and metabolites in human biological fluids. Concomitantly, an UHPLC-HRMS method was applied to investigate potential metabolites, assisted by a complementary GC-MS method to elucidate the presence of plausible pesticides co-formulants. The LC-ESI-MS/MS method exhibited acceptable mean recoveries at the lower limit of quantification (LLOQ) and three additional levels, varying from 85 to 106% for all analytes and matrices. In serum, urine, and gastric fluid samples, the suspect compounds, namely chlorpyrifos and myclobutanil, predominated. Gastric fluid samples contained the highest concentrations of chlorpyrifos (39,800 ng/mL) and myclobutanil (18,800 ng/mL), while the neonicotinoid imidacloprid was also quantified, below 30 ng/mL. Notwithstanding, the UHPLC-HRMS analysis unveiled several metabolites of chlorpyrifos and myclobutanil. In parallel, GC-MS analysis, corroborated the presence of several co-formulants in gastric fluid samples, exemplified by m- and o-xylene, and cyclohexanone. Overall, three analytical methods were implemented to elucidate the chemical causality of a human intoxication incident. The presence of suspected active substances, one additional, and several metabolites and co-formulants were documented.Kasiotis Konstantinos M.Baira EiriniManea-Karga ElectraNikolopoulou DimitraGanas KonstantinosMachera KyriakiDe Gruyterarticlepesticidesmetaboliteslc-esi-ms/msuhplc-hrmsbiological fluidsChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 107-118 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pesticides metabolites lc-esi-ms/ms uhplc-hrms biological fluids Chemistry QD1-999
spellingShingle	pesticides metabolites lc-esi-ms/ms uhplc-hrms biological fluids Chemistry QD1-999 Kasiotis Konstantinos M. Baira Eirini Manea-Karga Electra Nikolopoulou Dimitra Ganas Konstantinos Machera Kyriaki Investigating a human pesticide intoxication incident: The importance of robust analytical approaches
description	A human intoxication incident attributed to pesticide abuse was investigated using cutting-edge analytical methodologies. An LC-ESI-MS/MS method, based on a hybrid solid-phase extraction protocol (hybrid-SPE), was applied for the detection and quantification of several pesticides and metabolites in human biological fluids. Concomitantly, an UHPLC-HRMS method was applied to investigate potential metabolites, assisted by a complementary GC-MS method to elucidate the presence of plausible pesticides co-formulants. The LC-ESI-MS/MS method exhibited acceptable mean recoveries at the lower limit of quantification (LLOQ) and three additional levels, varying from 85 to 106% for all analytes and matrices. In serum, urine, and gastric fluid samples, the suspect compounds, namely chlorpyrifos and myclobutanil, predominated. Gastric fluid samples contained the highest concentrations of chlorpyrifos (39,800 ng/mL) and myclobutanil (18,800 ng/mL), while the neonicotinoid imidacloprid was also quantified, below 30 ng/mL. Notwithstanding, the UHPLC-HRMS analysis unveiled several metabolites of chlorpyrifos and myclobutanil. In parallel, GC-MS analysis, corroborated the presence of several co-formulants in gastric fluid samples, exemplified by m- and o-xylene, and cyclohexanone. Overall, three analytical methods were implemented to elucidate the chemical causality of a human intoxication incident. The presence of suspected active substances, one additional, and several metabolites and co-formulants were documented.
format	article
author	Kasiotis Konstantinos M. Baira Eirini Manea-Karga Electra Nikolopoulou Dimitra Ganas Konstantinos Machera Kyriaki
author_facet	Kasiotis Konstantinos M. Baira Eirini Manea-Karga Electra Nikolopoulou Dimitra Ganas Konstantinos Machera Kyriaki
author_sort	Kasiotis Konstantinos M.
title	Investigating a human pesticide intoxication incident: The importance of robust analytical approaches
title_short	Investigating a human pesticide intoxication incident: The importance of robust analytical approaches
title_full	Investigating a human pesticide intoxication incident: The importance of robust analytical approaches
title_fullStr	Investigating a human pesticide intoxication incident: The importance of robust analytical approaches
title_full_unstemmed	Investigating a human pesticide intoxication incident: The importance of robust analytical approaches
title_sort	investigating a human pesticide intoxication incident: the importance of robust analytical approaches
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/826f8340625f430ab96760771f200572
work_keys_str_mv	AT kasiotiskonstantinosm investigatingahumanpesticideintoxicationincidenttheimportanceofrobustanalyticalapproaches AT bairaeirini investigatingahumanpesticideintoxicationincidenttheimportanceofrobustanalyticalapproaches AT maneakargaelectra investigatingahumanpesticideintoxicationincidenttheimportanceofrobustanalyticalapproaches AT nikolopouloudimitra investigatingahumanpesticideintoxicationincidenttheimportanceofrobustanalyticalapproaches AT ganaskonstantinos investigatingahumanpesticideintoxicationincidenttheimportanceofrobustanalyticalapproaches AT macherakyriaki investigatingahumanpesticideintoxicationincidenttheimportanceofrobustanalyticalapproaches
_version_	1718371795187269632

Investigating a human pesticide intoxication incident: The importance of robust analytical approaches

Ejemplares similares