Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells.
During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorde...
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2010
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oai:doaj.org-article:827a5b4abcd4438882c24098f5f195752021-12-02T20:20:32ZHuman endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells.1932-620310.1371/journal.pone.0010964https://doaj.org/article/827a5b4abcd4438882c24098f5f195752010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20585575/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC.Irene CervellóIrene CervellóClaudia Gil-SanchisAymara MasFrancisco Delgado-RosasJosé Antonio Martínez-ConejeroAmparo GalánAlicia Martínez-RomeroSebastian MartínezIsmael NavarroJaime FerroJosé Antonio HorcajadasFrancisco José EstebanJosé Enrique O'ConnorAntonio PellicerCarlos SimónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e10964 (2010) |
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Medicine R Science Q |
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Medicine R Science Q Irene Cervelló Irene Cervelló Claudia Gil-Sanchis Aymara Mas Francisco Delgado-Rosas José Antonio Martínez-Conejero Amparo Galán Alicia Martínez-Romero Sebastian Martínez Ismael Navarro Jaime Ferro José Antonio Horcajadas Francisco José Esteban José Enrique O'Connor Antonio Pellicer Carlos Simón Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| description |
During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is the basis for women's cycling and its dysfunction may be involved in the etiology of pathological disorders. Therefore, the human endometrial tissue must rely on a remarkable endometrial somatic stem cells (SSC) population. Here we explore the hypothesis that human endometrial side population (SP) cells correspond to somatic stem cells. We isolated, identified and characterized the SP corresponding to the stromal and epithelial compartments using endometrial SP genes signature, immunophenotyping and characteristic telomerase pattern. We analyzed the clonogenic activity of SP cells under hypoxic conditions and the differentiation capacity in vitro to adipogenic and osteogenic lineages. Finally, we demonstrated the functional capability of endometrial SP to develop human endometrium after subcutaneous injection in NOD-SCID mice. Briefly, SP cells of human endometrium from epithelial and stromal compartments display genotypic, phenotypic and functional features of SSC. |
| format |
article |
| author |
Irene Cervelló Irene Cervelló Claudia Gil-Sanchis Aymara Mas Francisco Delgado-Rosas José Antonio Martínez-Conejero Amparo Galán Alicia Martínez-Romero Sebastian Martínez Ismael Navarro Jaime Ferro José Antonio Horcajadas Francisco José Esteban José Enrique O'Connor Antonio Pellicer Carlos Simón |
| author_facet |
Irene Cervelló Irene Cervelló Claudia Gil-Sanchis Aymara Mas Francisco Delgado-Rosas José Antonio Martínez-Conejero Amparo Galán Alicia Martínez-Romero Sebastian Martínez Ismael Navarro Jaime Ferro José Antonio Horcajadas Francisco José Esteban José Enrique O'Connor Antonio Pellicer Carlos Simón |
| author_sort |
Irene Cervelló |
| title |
Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| title_short |
Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| title_full |
Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| title_fullStr |
Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| title_full_unstemmed |
Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| title_sort |
human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/827a5b4abcd4438882c24098f5f19575 |
| work_keys_str_mv |
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