Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows

Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determ...

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Autores principales:	Pajić Marija, Boboš Stanko, Velebit Branko, Rašić Zoran, Katić Vera, Radinović Miodrag, Nikolić Aleksandra, Simonović Dušan, Babić Milijana
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	s. aureus mastitis staphylococcal enterotoxins phylogenetic relatedness Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/827a9beb783c45b5b04e488f860cefc2
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spelling	oai:doaj.org-article:827a9beb783c45b5b04e488f860cefc22021-11-17T21:27:51ZPrevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows1820-744810.1515/acve-2016-0040https://doaj.org/article/827a9beb783c45b5b04e488f860cefc22016-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0040https://doaj.org/toc/1820-7448Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confi rmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms.Pajić MarijaBoboš StankoVelebit BrankoRašić ZoranKatić VeraRadinović MiodragNikolić AleksandraSimonović DušanBabić MilijanaSciendoarticles. aureusmastitisstaphylococcal enterotoxinsphylogenetic relatednessVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 4, Pp 466-477 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	s. aureus mastitis staphylococcal enterotoxins phylogenetic relatedness Veterinary medicine SF600-1100
spellingShingle	s. aureus mastitis staphylococcal enterotoxins phylogenetic relatedness Veterinary medicine SF600-1100 Pajić Marija Boboš Stanko Velebit Branko Rašić Zoran Katić Vera Radinović Miodrag Nikolić Aleksandra Simonović Dušan Babić Milijana Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows
description	Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confi rmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms.
format	article
author	Pajić Marija Boboš Stanko Velebit Branko Rašić Zoran Katić Vera Radinović Miodrag Nikolić Aleksandra Simonović Dušan Babić Milijana
author_facet	Pajić Marija Boboš Stanko Velebit Branko Rašić Zoran Katić Vera Radinović Miodrag Nikolić Aleksandra Simonović Dušan Babić Milijana
author_sort	Pajić Marija
title	Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows
title_short	Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows
title_full	Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows
title_fullStr	Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows
title_full_unstemmed	Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows
title_sort	prevalence and molecular characterization of enterotoxin-producing strains of staphylococcus aureus isolated from serbian dairy cows
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/827a9beb783c45b5b04e488f860cefc2
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Prevalence and Molecular Characterization of Enterotoxin-Producing Strains of Staphylococcus Aureus Isolated from Serbian Dairy Cows

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