Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense

Abstract Recent high annual losses of honey bee colonies are associated with many factors, including RNA virus infections. Honey bee antiviral responses include RNA interference and immune pathway activation, but their relative roles in antiviral defense are not well understood. To better characteri...

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Autores principales:	Laura M. Brutscher, Katie F. Daughenbaugh, Michelle L. Flenniken
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/827c2b14addc4b71b1fbb31261abbadd
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spelling	oai:doaj.org-article:827c2b14addc4b71b1fbb31261abbadd2021-12-02T15:05:32ZVirus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense10.1038/s41598-017-06623-z2045-2322https://doaj.org/article/827c2b14addc4b71b1fbb31261abbadd2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06623-zhttps://doaj.org/toc/2045-2322Abstract Recent high annual losses of honey bee colonies are associated with many factors, including RNA virus infections. Honey bee antiviral responses include RNA interference and immune pathway activation, but their relative roles in antiviral defense are not well understood. To better characterize the mechanism(s) of honey bee antiviral defense, bees were infected with a model virus in the presence or absence of dsRNA, a virus associated molecular pattern. Regardless of sequence specificity, dsRNA reduced virus abundance. We utilized next generation sequencing to examine transcriptional responses triggered by virus and dsRNA at three time-points post-infection. Hundreds of genes exhibited differential expression in response to co-treatment of dsRNA and virus. Virus-infected bees had greater expression of genes involved in RNAi, Toll, Imd, and JAK-STAT pathways, but the majority of differentially expressed genes are not well characterized. To confirm the virus limiting role of two genes, including the well-characterized gene, dicer, and a probable uncharacterized cyclin dependent kinase in honey bees, we utilized RNAi to reduce their expression in vivo and determined that virus abundance increased, supporting their involvement in antiviral defense. Together, these results further our understanding of honey bee antiviral defense, particularly the role of a non-sequence specific dsRNA-mediated antiviral pathway.Laura M. BrutscherKatie F. DaughenbaughMichelle L. FlennikenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura M. Brutscher Katie F. Daughenbaugh Michelle L. Flenniken Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense
description	Abstract Recent high annual losses of honey bee colonies are associated with many factors, including RNA virus infections. Honey bee antiviral responses include RNA interference and immune pathway activation, but their relative roles in antiviral defense are not well understood. To better characterize the mechanism(s) of honey bee antiviral defense, bees were infected with a model virus in the presence or absence of dsRNA, a virus associated molecular pattern. Regardless of sequence specificity, dsRNA reduced virus abundance. We utilized next generation sequencing to examine transcriptional responses triggered by virus and dsRNA at three time-points post-infection. Hundreds of genes exhibited differential expression in response to co-treatment of dsRNA and virus. Virus-infected bees had greater expression of genes involved in RNAi, Toll, Imd, and JAK-STAT pathways, but the majority of differentially expressed genes are not well characterized. To confirm the virus limiting role of two genes, including the well-characterized gene, dicer, and a probable uncharacterized cyclin dependent kinase in honey bees, we utilized RNAi to reduce their expression in vivo and determined that virus abundance increased, supporting their involvement in antiviral defense. Together, these results further our understanding of honey bee antiviral defense, particularly the role of a non-sequence specific dsRNA-mediated antiviral pathway.
format	article
author	Laura M. Brutscher Katie F. Daughenbaugh Michelle L. Flenniken
author_facet	Laura M. Brutscher Katie F. Daughenbaugh Michelle L. Flenniken
author_sort	Laura M. Brutscher
title	Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense
title_short	Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense
title_full	Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense
title_fullStr	Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense
title_full_unstemmed	Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense
title_sort	virus and dsrna-triggered transcriptional responses reveal key components of honey bee antiviral defense
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/827c2b14addc4b71b1fbb31261abbadd
work_keys_str_mv	AT laurambrutscher virusanddsrnatriggeredtranscriptionalresponsesrevealkeycomponentsofhoneybeeantiviraldefense AT katiefdaughenbaugh virusanddsrnatriggeredtranscriptionalresponsesrevealkeycomponentsofhoneybeeantiviraldefense AT michellelflenniken virusanddsrnatriggeredtranscriptionalresponsesrevealkeycomponentsofhoneybeeantiviraldefense
_version_	1718388776261124096

Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense

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