Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>

In the agricultural field, blanching is a technique used to obtain tender, sweet, and delicious water dropwort stems by blocking sunlight. The physiological and nutritional parameters of blanched water dropwort have been previously investigated. However, the molecular mechanism of blanching remains...

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Autores principales:	Sunjeet Kumar, Xinfang Huang, Gaojie Li, Qun Ji, Kai Zhou, Guopeng Zhu, Weidong Ke, Hongwei Hou, Honglian Zhu, Jingjing Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	blanching transcriptome photosynthesis plant hormones transcription factors Botany QK1-989
Acceso en línea:	https://doaj.org/article/829ae6f185af44b0abf9fbbfd4796c87
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spelling	oai:doaj.org-article:829ae6f185af44b0abf9fbbfd4796c872021-11-25T18:46:59ZComparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>10.3390/plants101124842223-7747https://doaj.org/article/829ae6f185af44b0abf9fbbfd4796c872021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2484https://doaj.org/toc/2223-7747In the agricultural field, blanching is a technique used to obtain tender, sweet, and delicious water dropwort stems by blocking sunlight. The physiological and nutritional parameters of blanched water dropwort have been previously investigated. However, the molecular mechanism of blanching remains unclear. In the present study, we investigated transcriptomic variations for different blanching periods in the stem of water dropwort (pre, mid, post-blanching, and control). The results showed that many genes in pathways, such as photosynthesis, carbon fixation, and phytohormone signal transduction as well as transcription factors (TFs) were significantly dysregulated. Blanched stems of water dropwort showed the higher number of downregulated genes in pathways, such as photosynthesis, antenna protein, carbon fixation in photosynthetic organisms, and porphyrin and chlorophyll metabolism, which ultimately affect the photosynthesis in water dropwort. The genes of hormone signal transduction pathways (ethylene, jasmonic acid, brassinosteroid, and indole-3-acetic acid) showed upregulation in the post-blanched water dropwort plants. Overall, a higher number of genes coding for TFs, such as ERF, BHLH, MYB, zinc-finger, bZIP, and WRKY were overexpressed in blanched samples in comparison with the control. These genes and pathways participate in inducing the length, developmental processes, pale color, and stress tolerance of the blanched stem. Overall, the genes responsive to blanching, which were identified in this study, provide an effective foundation for further studies on the molecular mechanisms of blanching and photosynthesis regulations in water dropwort and other species.Sunjeet KumarXinfang HuangGaojie LiQun JiKai ZhouGuopeng ZhuWeidong KeHongwei HouHonglian ZhuJingjing YangMDPI AGarticleblanchingtranscriptomephotosynthesisplant hormonestranscription factorsBotanyQK1-989ENPlants, Vol 10, Iss 2484, p 2484 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	blanching transcriptome photosynthesis plant hormones transcription factors Botany QK1-989
spellingShingle	blanching transcriptome photosynthesis plant hormones transcription factors Botany QK1-989 Sunjeet Kumar Xinfang Huang Gaojie Li Qun Ji Kai Zhou Guopeng Zhu Weidong Ke Hongwei Hou Honglian Zhu Jingjing Yang Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>
description	In the agricultural field, blanching is a technique used to obtain tender, sweet, and delicious water dropwort stems by blocking sunlight. The physiological and nutritional parameters of blanched water dropwort have been previously investigated. However, the molecular mechanism of blanching remains unclear. In the present study, we investigated transcriptomic variations for different blanching periods in the stem of water dropwort (pre, mid, post-blanching, and control). The results showed that many genes in pathways, such as photosynthesis, carbon fixation, and phytohormone signal transduction as well as transcription factors (TFs) were significantly dysregulated. Blanched stems of water dropwort showed the higher number of downregulated genes in pathways, such as photosynthesis, antenna protein, carbon fixation in photosynthetic organisms, and porphyrin and chlorophyll metabolism, which ultimately affect the photosynthesis in water dropwort. The genes of hormone signal transduction pathways (ethylene, jasmonic acid, brassinosteroid, and indole-3-acetic acid) showed upregulation in the post-blanched water dropwort plants. Overall, a higher number of genes coding for TFs, such as ERF, BHLH, MYB, zinc-finger, bZIP, and WRKY were overexpressed in blanched samples in comparison with the control. These genes and pathways participate in inducing the length, developmental processes, pale color, and stress tolerance of the blanched stem. Overall, the genes responsive to blanching, which were identified in this study, provide an effective foundation for further studies on the molecular mechanisms of blanching and photosynthesis regulations in water dropwort and other species.
format	article
author	Sunjeet Kumar Xinfang Huang Gaojie Li Qun Ji Kai Zhou Guopeng Zhu Weidong Ke Hongwei Hou Honglian Zhu Jingjing Yang
author_facet	Sunjeet Kumar Xinfang Huang Gaojie Li Qun Ji Kai Zhou Guopeng Zhu Weidong Ke Hongwei Hou Honglian Zhu Jingjing Yang
author_sort	Sunjeet Kumar
title	Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>
title_short	Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>
title_full	Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>
title_fullStr	Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>
title_full_unstemmed	Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>
title_sort	comparative transcriptomic analysis provides novel insights into the blanched stem of <i>oenanthe javanica</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/829ae6f185af44b0abf9fbbfd4796c87
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Comparative Transcriptomic Analysis Provides Novel Insights into the Blanched Stem of <i>Oenanthe javanica</i>

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