Role of the gut endoderm in relaying left-right patterning in mice.

Establishment of left-right (LR) asymmetry occurs after gastrulation commences and utilizes a conserved cascade of events. In the mouse, LR symmetry is broken at a midline structure, the node, and involves signal relay to the lateral plate, where it results in asymmetric organ morphogenesis. How inf...

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Autores principales:	Manuel Viotti, Lei Niu, Song-Hai Shi, Anna-Katerina Hadjantonakis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/829e6d25c6c84ef39ac2880c4ecac8f4
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spelling	oai:doaj.org-article:829e6d25c6c84ef39ac2880c4ecac8f42021-11-18T05:36:44ZRole of the gut endoderm in relaying left-right patterning in mice.1544-91731545-788510.1371/journal.pbio.1001276https://doaj.org/article/829e6d25c6c84ef39ac2880c4ecac8f42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22412348/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Establishment of left-right (LR) asymmetry occurs after gastrulation commences and utilizes a conserved cascade of events. In the mouse, LR symmetry is broken at a midline structure, the node, and involves signal relay to the lateral plate, where it results in asymmetric organ morphogenesis. How information transmits from the node to the distantly situated lateral plate remains unclear. Noting that embryos lacking Sox17 exhibit defects in both gut endoderm formation and LR patterning, we investigated a potential connection between these two processes. We observed an endoderm-specific absence of the critical gap junction component, Connexin43 (Cx43), in Sox17 mutants. Iontophoretic dye injection experiments revealed planar gap junction coupling across the gut endoderm in wild-type but not Sox17 mutant embryos. They also revealed uncoupling of left and right sides of the gut endoderm in an isolated domain of gap junction intercellular communication at the midline, which in principle could function as a barrier to communication between the left and right sides of the embryo. The role for gap junction communication in LR patterning was confirmed by pharmacological inhibition, which molecularly recapitulated the mutant phenotype. Collectively, our data demonstrate that Cx43-mediated communication across gap junctions within the gut endoderm serves as a mechanism for information relay between node and lateral plate in a process that is critical for the establishment of LR asymmetry in mice.Manuel ViottiLei NiuSong-Hai ShiAnna-Katerina HadjantonakisPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 10, Iss 3, p e1001276 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Manuel Viotti Lei Niu Song-Hai Shi Anna-Katerina Hadjantonakis Role of the gut endoderm in relaying left-right patterning in mice.
description	Establishment of left-right (LR) asymmetry occurs after gastrulation commences and utilizes a conserved cascade of events. In the mouse, LR symmetry is broken at a midline structure, the node, and involves signal relay to the lateral plate, where it results in asymmetric organ morphogenesis. How information transmits from the node to the distantly situated lateral plate remains unclear. Noting that embryos lacking Sox17 exhibit defects in both gut endoderm formation and LR patterning, we investigated a potential connection between these two processes. We observed an endoderm-specific absence of the critical gap junction component, Connexin43 (Cx43), in Sox17 mutants. Iontophoretic dye injection experiments revealed planar gap junction coupling across the gut endoderm in wild-type but not Sox17 mutant embryos. They also revealed uncoupling of left and right sides of the gut endoderm in an isolated domain of gap junction intercellular communication at the midline, which in principle could function as a barrier to communication between the left and right sides of the embryo. The role for gap junction communication in LR patterning was confirmed by pharmacological inhibition, which molecularly recapitulated the mutant phenotype. Collectively, our data demonstrate that Cx43-mediated communication across gap junctions within the gut endoderm serves as a mechanism for information relay between node and lateral plate in a process that is critical for the establishment of LR asymmetry in mice.
format	article
author	Manuel Viotti Lei Niu Song-Hai Shi Anna-Katerina Hadjantonakis
author_facet	Manuel Viotti Lei Niu Song-Hai Shi Anna-Katerina Hadjantonakis
author_sort	Manuel Viotti
title	Role of the gut endoderm in relaying left-right patterning in mice.
title_short	Role of the gut endoderm in relaying left-right patterning in mice.
title_full	Role of the gut endoderm in relaying left-right patterning in mice.
title_fullStr	Role of the gut endoderm in relaying left-right patterning in mice.
title_full_unstemmed	Role of the gut endoderm in relaying left-right patterning in mice.
title_sort	role of the gut endoderm in relaying left-right patterning in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/829e6d25c6c84ef39ac2880c4ecac8f4
work_keys_str_mv	AT manuelviotti roleofthegutendoderminrelayingleftrightpatterninginmice AT leiniu roleofthegutendoderminrelayingleftrightpatterninginmice AT songhaishi roleofthegutendoderminrelayingleftrightpatterninginmice AT annakaterinahadjantonakis roleofthegutendoderminrelayingleftrightpatterninginmice
_version_	1718424859995799552

Role of the gut endoderm in relaying left-right patterning in mice.

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